2018 Fiscal Year Final Research Report
Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatization
| Project/Area Number |
15K21662
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
Analytical chemistry
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| Research Institution | Fukuoka University (2018)
National Institute of Advanced Industrial Science and Technology (2015-2017) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Keywords | LC-MS / タンパク質 / ペプチド / アミノ酸 / 誘導体化 / カルボキシル基 |
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| Outline of Final Research Achievements |
In this study, a carboxyl groups derivatization LC-MS method for proteins or peptides was developed. By derivatizing the highly polar carboxyl group, it was possible to efficiently improve the sensitivity in LC-MS analysis. Furthermore, deamidation, which is a major degradation of proteins, is considered difficult to evaluate in LC-MS analysis because the mass hardly changes before and after degradation. However using this method it was possible to change the structure clearly before and after deterioration, and it was possible to evaluate easily.
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| Free Research Field |
分析化学
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| Academic Significance and Societal Importance of the Research Achievements |
新規臨床検査項目や新規バイオ医薬品が年々増加しているなかで，それらを安全に信頼して利用していただくためにも，タンパク質の構造変化をとらえる分析手法が必須だと考える。本研究において開発した分析法は、LC-MS分析におけるタンパク質またはペプチドの高感度化と、これまで正確な評価が困難だった脱アミド化を容易に評価することを可能とした。
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