Creation of Synthetic Bacteriophages -Treatment of Drug-Resistant Bacterial Infections and Human Microbiota Editing-

2022 Fiscal Year Final Research Report

• Project/Area Number: 15K21770
• Research Category: Fund for the Promotion of Joint International Research (Home-Returning Researcher Development Research)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Gifu University
• Principal Investigator: ANDO Hiroki 岐阜大学, 大学院医学系研究科, 特任准教授 (70462786)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Keywords: バクテリオファージ / 合成生物学 / ファージ療法 / ファージセラピー

Outline of Final Research Achievements

The "Synthetic Engineering Platform" was developed. The platform can construct bacteriophage genomes from DNA fragments and reboot functional phages. A variety of synthetic phages was created. In addition, biological containment methods were developed to enable the use of synthetic phages in, for example, phage therapy and microbiota editing. Biocontained phages showed therapeutic effects comparable to those of wild-type phages in phage therapy experiments. I have filed a patent application and published a paper on the results of this research.

Free Research Field

合成生物学

Academic Significance and Societal Importance of the Research Achievements

様々なファージを改変して起動できるプラットフォームは、ファージの基礎研究・応用研究を推進する。また、ファージの生物学的封じ込めは、例えば、改変型ファージの研究室外での利用を考えた際に有力な拡散防止策になりえる。本プラットフォームと生物学的封じ込め法はファージ生物学と関連分野における新しい基盤技術になるものと考えている。