In-cell signal amplification system developed by evolutionally molecular engineering(Fostering Joint International Research)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KK0190
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: UZAWA Takanori 国立研究開発法人理化学研究所, 創発物性科学研究センター, 専任研究員 (60554376)
• Research Collaborator: Hollfelder Florian University of Cambridge, Department of Biochemistry, Prof.
• Project Period (FY): 2016 – 2017
• Keywords: 非天然アミノ酸 / アミノアシルtRNA合成酵素

Outline of Final Research Achievements

As an extension form my primary subject that aimed to develop an in-cell signal amplification system using light as a trigger, I aimed to establish a system to introduce a fluorescence amino acid into a protein. First I established systems to screen aminoacyl-tRNA synthetases using E.coli and beads display that was acquired at University of Cambridge. Next, I confirmed that the system works using an unnatural amino acid that have been reported before. Then, using these systems, I obtained candidate mutants that would charge a fluorescent amino acid.

Free Research Field

進化分子工学