Whole genome manipulation for understanding of biology of unculturable bacteria(Fostering Joint International Research)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KK0266
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: kakizawa shigeyuki 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10588669)
• Research Collaborator: Glass John The J. Craig Venter Institute, Synthetic Biology & Bioenergy group, Professor ; Suzuki Yo The J. Craig Venter Institute, Synthetic Biology & Bioenergy group, Assistant Professor ; Wise Kim The J. Craig Venter Institute, Synthetic Biology & Bioenergy group, Senior Scientist
• Project Period (FY): 2016 – 2017
• Keywords: 細菌 / ゲノム / 合成生物学

Outline of Final Research Achievements

The aim of this project is establishing novel techniques to handle large genomic DNA fragments and to analyze biology of unculturable bacteria. In this project, I used a new plasmid vector that could be replicate in mycoplasma cells, and tried to introduce large DNA fragment into the plasmid vector. In this way, there are less risks of shearing of large DNA molecules during handling. First, I analyzed several characteristics of this plasmid, e.g., replication efficiency, effective transformation methods, etc. Next, I tried to modify the plasmid in Yeast cells, since the vector could be introduced into Yeast cells.

Free Research Field

細菌学