Preparation of functional glyco-polymers and analysis of their cellular immune activities to develop anti-pollinosis drugs(Fostering Joint International Research)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KK0282
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Okayama University
• Principal Investigator: MAEDA Megumi 岡山大学, 環境生命科学研究科, 准教授 (20434988)
• Research Collaborator: VAN DAMME Els J.M. Faculty of Bioscience Engineering, Ghent University, Department of Molecular Biotechnology, Prof. ; Rahman Ziaur Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission
• Project Period (FY): 2015 – 2017
• Keywords: 遊離N-グリカン / 糖鎖結合タンパク質 / レクチン / 糖鎖ポリマー / PNGase / ENGase / 小胞体品質管理 / ルイスa抗原

Outline of Final Research Achievements

Naturally occurring free N-glycans (FNGs) derived from glycoproteins/glycopeptides by the action of peptide: N-glycanase (PNGase) and endo-β-N-acetylglucosaminidase (ENGase) have been postulated to act as signaling molecules stimulating plant growth or fruit ripening. To elucidate the physiological role of FNGs in plants, we have analyzed the sensitivity of the A.thaliana mutants, ENGase-DKO and acidic PNGase-DKO, to abiotic (NaCl treatment) and biotic (P. syringae infection) stresses. Since it is believed that nucleocytoplasmic lectins function in the plant stress response, synthesized glycopolymers were used to study the interaction between FNGs and lectins in plant cells.

Free Research Field

糖鎖生物学，生化学，免疫学