Selective gene expression machinary during prolonged phase of hypoxia(Fostering Joint International Research)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KK0298
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NAKAYAMA Koh 東京医科歯科大学, 難治疾患研究所, 准教授 (10451923)
• Research Collaborator: Misteli Tom 国立がん研究所, NIH Distinguished Investigator
• Project Period (FY): 2016 – 2017
• Keywords: 低酸素応答 / 遺伝子発現 / 転写 / 染色体構造

Outline of Final Research Achievements

We investigated the molecular machinery of gene expression during acute and prolonged phase of hypoxia by focusing on its nuclear position. We first picked up about 200 genes which is up-regulated during hypoxic response in a HIF- or CREB-dependent manner. Probes for these genes were synthesized and their nuclear positions were determined by HIPMap method. This analysis indicated that multiple genes which change expressions in hypoxia, also change their nuclear position. These results indicate that dynamic changes in the gene expression during hypoxia are also co-related with their nuclear positions.

Free Research Field

分子医科学