Cell lineage analysis of changes in characteristics of pancreatic cancer cells at single cell level(Fostering Joint International Research)

2018 Fiscal Year Final Research Report

• Project/Area Number: 15KK0314
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Yokohama City University
• Principal Investigator: Sekine Keisuke 横浜市立大学, 医学部, 講師 (00323569)
• Research Collaborator: CAMP J. Gray Max Planck Institute for Evolutionary Anthropology, Department of Evolutionary Genetics, Group leader ; TREUTLEIN Barbara Max Planck Institute for Evolutionary Anthropology, Max Planck Research Group on Single Cell Genomics, Group leader and Assistant Professor at TU Munich
• Project Period (FY): 2016 – 2018
• Keywords: オルガノイド

Outline of Final Research Achievements

In this study, single-cell RNA sequencing of human three-dimensional organs was carried out with Dr. Camp and Dr. Treutlein others under at Max Planck Institute, Germany.
We identified molecules whose expression could only be seen when multiple cell types were mixed together to generate organoid. By analyzing in detail, we identified signal candidates involved in cell-cell interactions. We performed functional analysis based on the information of this signal candidate molecule and revealed that multiple signal candidates actually contribute to steric intercellular interaction (Camp JG, Sekine K et al, Nature 2017).

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究によって、生体内で見られる複数の細胞種により構成される立体的な組織における細胞間相互作用の一旦を明らかにし、従来の平面培養における課題を明らかにした。また、本手法によって立体的な細胞間相互作用の新たな解析手法を提示することが出来た。これらの成果は、幹細胞生物学を背景に立体臓器の再構成技術をもつ日本側研究者とシングルセルRNAシークエンス解析の先駆者であるドイツ側研究者の国際共同研究によってはじめて成しえた成果と言える。