Identification and characterization of microbial drivers involved in nitrous oxide gas emission from upland field

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KT0024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Food Cycle Research
• Research Institution: The University of Tokyo
• Principal Investigator: Senoo Keishi 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)
• Co-Investigator(Kenkyū-buntansha): 磯部 一夫 東京大学, 大学院農学生命科学研究科(農学部), 助教 (30621833)
• Co-Investigator(Renkei-kenkyūsha): OTSUKA Shigeto 東京大学, 農学生命科学研究科, 准教授 (10313074)
• Research Collaborator: SHIRATORI Yutaka 新潟県農業総合研究所, 基盤研究部, 専門研究員 ; KAKUTA Hiroko 東京大学, 農学生命科学研究科 ; MASUDA Yoko 東京大学, 農学生命科学研究科 ; MASAKI Katsuya 東京大学, 農学生命科学研究科
• Project Period (FY): 2015-07-10 – 2018-03-31
• Keywords: 一酸化二窒素ガス / 畑土壌 / 土壌微生物 / 環境調和型農業 / 土壌学

Outline of Final Research Achievements

Large emission of nitrous oxide (N2O) gas is observed in upland field soil due to application of nitrogen fertilizer. To mitigate the N2O emission, identification and characterization of the microbial drivers of N2O generation and elimination in soils are essential. In this study, we successfully identify N2O generating/eliminating microorganisms in soil based on the analysis of soil DNA/RNA and bacterial isolates. N2O eliminating activity of large variety of bacterial isolates was enhanced by addition of Cu to culture medium. Based on the genome information, mechanism of plant growth promoting activity of two strains of N2O eliminating bacteria was clarified. These results will be useful to develop novel bio-fertilizer with both N2O mitigation and plant growth promoting ability.

Free Research Field

土壌学