Elucidation of identity of cell membranes by sythetic biological approaches

2017 Fiscal Year Final Research Report

• Project/Area Number: 15KT0152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Constructive Systems Biology
• Research Institution: Kyushu University
• Principal Investigator: Ikenouchi Junichi 九州大学, 理学研究院, 教授 (10500051)
• Project Period (FY): 2015-07-10 – 2018-03-31
• Keywords: リポソーム / エレクトロポレーション / 細胞膜 / 膜タンパク質 / 細胞質 / 細胞質流動性 / ブレブ / 細胞小器官

Outline of Final Research Achievements

Various lipids and membrane are assumed as candidates for molecules that define the identity of cell membranes such as plasma membrane and organelles. However, it is difficult to determine the minimal combination of lipids and membrane proteins which define specific cell membrane by conventional cell biological approaches. Therefore, we tried to establish a method to introduce liposomes and proteins into cells in this research project. As a result, we established a method to introduce liposome and protein complex encapsulated in giant liposomes into cells by fusing cells with giant liposomes by electroporation.

Free Research Field

細胞生物学