Structural study of signal transduction through membrane proteins, channels and receptors

2008 Fiscal Year Final Research Report

• Project/Area Number: 16001005
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: FUJIYOSHI Yoshinori Kyoto University, 大学院・理学研究科, 教授 (80142298)
• Co-Investigator(Kenkyū-buntansha): MIZOGUCHI Akira 三重大学, 医学部, 教授 (90181916) ; YASUI Masato 慶應義塾大学, 医学部, 教授 (90246637)
• Project Period (FY): 2004 – 2008
• Keywords: 構造生物学 / シグナル伝達 / 神経科学 / 生理学 / 脳・神経

Research Abstract

膜蛋白質を中心とする情報伝達と制御の分子機構を構造の視点から理解することを目的として、具体的には、1) 水チャネルの構造と機能、活性制御、そして高次機能の研究、2) イオンチャネルの構造と機能解析および局在化機構等による高次機能の解析、3) エンドセリン受容体B型(ETBR)等のG蛋白質共役型受容体(GPCR)の構造と機能解析の3つの研究課題を遂行した。

Research Products

• [Journal Article] Mechanism of Aquaporin-4's Fast and Highly Selective Water Conduction and Proton Exclusion. (2009)
  • Author(s): K. Tani, T. Mitsuma, Y. Hiroaki, A. Kamegawa, K. Nishikawa, Y. Tanimura and Y. Fujiyoshi
  • Journal Title: J. Mol. Biol. 389 Pages: 694-706
  • Peer Reviewed
• [Journal Article] Inter-subunit interaction of gastric H^+, K^+-ATPase prevents reverse reaction of the transport cycle. (2009)
  • Author(s): K. Abe, K. Tani, T. Nishizawa and Y. Fujiyoshi
  • Journal Title: EMBO J. (in the press)
  • Peer Reviewed
• [Journal Article] Structure of the connexin-26 gap junction channel at 3.5 Å resolution. (2009)
  • Author(s): S. Maeda, S. Nakagawa, M. Suga, E. Yamashita, A. Oshima, Y. Fujiyoshi and T. Tsukihara
  • Journal Title: Nature 458 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Acetazolamide reversibly inhibits water conduction by aquaporin-4. (2009)
  • Author(s): Y. Tanimura, Y. Hiroaki and Y. Fujiyoshi
  • Journal Title: J. Struct. Biol. 166 Pages: 16-21
  • Peer Reviewed
• [Journal Article] Formation of aquaporin-4 arrays is inhibited by palmitoylation of N-terminal cysteine residues. (2008)
  • Author(s): H. Suzuki, K. Nishikawa, Y. Hiroaki and Y. Fujiyoshi
  • Journal Title: Biochem. Biophys. Acta. 1778 Pages: 1181-1189
  • Peer Reviewed
• [Journal Article] Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. (2007)
  • Author(s): A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi and G. E. Sosinsky
  • Journal Title: PNAS 104 Pages: 10034-10039
  • Peer Reviewed
• [Journal Article] Aquaporin-11 containing a divergent NPA motif has normal water channel activity. (2007)
  • Author(s): K. Yakata, Y. Hiroaki, K. Ishibashi, E. Sohara, S. Sasaki, K. Mitsuoka and Y. Fujiyoshi
  • Journal Title: Biochim. Biophys. Acta. 1768 Pages: 688-693
  • Peer Reviewed
• [Journal Article] Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitating presynaptic calcium currents at the calyx of Held of immature rats. (2006)
  • Author(s): T. Hige, Y. Fujiyoshi and T. Takahashi
  • Journal Title: Eur. J. Neurosci. 24 Pages: 1955-1966
  • Peer Reviewed
• [Journal Article] Implications of the Aquaporin-4 Structure on Array Formation and Cell Adhesion. (2006)
  • Author(s): Y. Hiroaki, K. Tani, A. Kamegawa, N. Gyobu, K. Nishikawa, H. Suzuki, T. Walz, S. Sasaki, K. Mitsuoka, K. Kimura, A. Mizoguchi and Y. Fujiyoshi
  • Journal Title: J. Mol. Biol. 355 Pages: 628-639
  • Peer Reviewed
• [Journal Article] Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. (2005)
  • Author(s): T. Gonen, Y. Cheng, P. Sliz, Y. Hiroaki, Y. Fujiyoshi, S. C. Harrison and T. Walz
  • Journal Title: Nature 438 Pages: 633-638
  • Peer Reviewed
• [Presentation] Structure and function of adhennels : adhesive channels (Invited lecture) (2009)
  • Author(s): Y. Fujiyoshi
  • Organizer: Network of Excelence-3D-EM Final Meeting
  • Place of Presentation: Brdo (Slovenia)
  • Year and Date: 20090209-13
• [Presentation] Structure and function of Multifunctional channels (Keynote lecture) (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: IUCr2008
  • Place of Presentation: Osaka (JAPAN)
  • Year and Date: 20080823-31
• [Presentation] Structure and Function of Multifunctional Channels (Special Lecture) (2008)
  • Author(s): Y. Fujiyoshi
  • Organizer: Neuroscience2008 (The 31st Annual Meeting of the Japan Neuroscience Society)
  • Place of Presentation: Tokyo (JAPAN)
  • Year and Date: 20080709-11
• [Presentation] Structure and function of aquaporins (Keynote lecture) (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara (JAPAN)
  • Year and Date: 20070713-16
• [Presentation] Structural physiology of multifunctional channels (Keynote lecture) (2007)
  • Author(s): Y. Fujiyoshi
  • Organizer: Joint International Symposium "Membrane Transport as a universal Biological mechanism"
  • Place of Presentation: Kyoto (JAPAN)
  • Year and Date: 20070113-14
• [Presentation] Structure and function of cell adhesive (2006)
  • Author(s): Y. Fujiyoshi
  • Organizer: 5th International NCCR Symposium on New Trends in Structural Biology
  • Place of Presentation: Zurich (SWITZERLAND)
  • Year and Date: 20060915-16
• [Presentation] Structure and function of various water channels (Keynote Lecture) (2005)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 2nd Bilateral Japan-UK Symposium on Structural Genomics and Proteomics
  • Place of Presentation: Yokohama (JAPAN)
  • Year and Date: 20050528-30
• [Presentation] Structure and function of water channels (2005)
  • Author(s): Y. Fujiyoshi
  • Organizer: 4th World Congress of Cellular and Molecular Biology
  • Place of Presentation: Poitiers (FRANCE)
  • Year and Date: 2005-10-09
• [Presentation] Structure and gating mechanism of the acetylcholine receptor (2004)
  • Author(s): Y. Fujiyoshi
  • Organizer: The 77th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Yokohama (JAPAN)
  • Year and Date: 20041013-16
• [Presentation] Structure and function of channels observed through an electron beam (Special Lecture) (2004)
  • Author(s): Y. Fujiyoshi
  • Organizer: 16th International Congress of the IFAA Anatomical Science 2004 -From Gene to Body-
  • Place of Presentation: Kyoto (JAPAN)
  • Year and Date: 20040822-27
• [Book] The AQP structure and functional implications. Handb. Exp. Pharmacol., 190 (2009)
  • Author(s): T. Walz, Y. Fujiyoshi and A. Engel
  • Total Pages: 31-56
  • Publisher: Springer-Verlag.
• [Book] pH Regulated Anion Permeability of Aquaporin-6. Handb. Exp. Pharmacol., 190 (2009)
  • Author(s): M. Yasui
  • Total Pages: 299-308
  • Publisher: Springer-Verlag.
• [Book] 膜タンパク質のナノバイオロジー チャネルの分子構造を機能 ナノバイオロジー ナノテクノロジーによる生命科学 第4章 (2004)
  • Author(s): 光岡薫, 藤吉好則
  • Total Pages: 37-47
  • Publisher: 共立出版
• [Remarks]
  • URL: http://em.biophys.kyoto-u.ac.jp/
• [Patent(Industrial Property Rights)] Specimen stage-moving device for charged-particle beam system (2008)
  • Inventor(s): Yoshinori Fujiyoshi, Tomohisa Fukuda
  • Industrial Property Rights Holder: JEOL Ltd.
  • Industrial Property Number: 特許08251272.4
  • Filing Date: 2008-03-31
  • Overseas
• [Patent(Industrial Property Rights)] Specimen stage-moving device for charged-particle beam system (2008)
  • Inventor(s): Yoshinori Fujiyoshi, Tomohisa Fukuda
  • Industrial Property Rights Holder: JEOL Ltd.
  • Industrial Property Number: 特許12/057835
  • Filing Date: 2008-03-28
  • Overseas
• [Patent(Industrial Property Rights)] 固体内部の組織学的イメージ像を観察・取得する方法 (2007)
  • Inventor(s): 溝口明, 楠木正人
  • Industrial Property Rights Holder: 三重大学
  • Industrial Property Number: 特願2007-129723
  • Filing Date: 2007-05-15
• [Patent(Industrial Property Rights)] 荷電粒子線装置の試料ステージ移動装置 (2007)
  • Inventor(s): 藤吉好則, 福田知久
  • Industrial Property Rights Holder: 京都大学, 日本電子(株)
  • Industrial Property Number: 特願2007-094862
  • Filing Date: 2007-03-30
• [Patent(Industrial Property Rights)] トップエントリ式試料ステージ傾斜装置 (2005)
  • Inventor(s): 藤吉好則, 出口俊二
  • Industrial Property Rights Holder: 京都大学, 日本電子(株)
  • Industrial Property Number: 特願2005-205558
  • Filing Date: 2005-07-14