Regulatory mechanisms of meiosis

2008 Fiscal Year Final Research Report

• Project/Area Number: 16002010
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAMOTO Masayuki The University of Tokyo, 大学院・理学系研究科, 教授 (40114706)
• Co-Investigator(Kenkyū-buntansha): TANAKA Kayoko 東京大学, 大学院・理学系研究科, 講師 (80345264) ; YAMASHITA Akira 東京大学, 大学院・理学系研究科, 助教授 (30312276) ; SATO Masamitsu 東京大学, 大学院・理学系研究科, 助教授 (50447356)
• Project Period (FY): 2004 – 2008
• Keywords: RNA / 細胞周期 / 減数分裂

Research Abstract

分裂酵母を材料に減数分裂を制御する分子機構を解析し、減数分裂のための遺伝子のmRNAにはそれらを栄養増殖時には細胞から選択的に取り除くための目印が埋め込まれていることを発見し、またそれらが減数分裂時には減数分裂マスター制御因子Mei2pによって安定化されるメカニズムを解明した。

Research Products

• [Journal Article] A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast. (2008)
  • Author(s): Kimata, Y., Trickey, M., Izawa, D., Gannon, J., Yamamoto, M., and Yamano, H.
  • Journal Title: Dev. Cell 14 Pages: 446-454
  • Peer Reviewed
• [Journal Article] TOR signaling in fission yeast. (2008)
  • Author(s): Otsubo, Y., and Yamamoto, M.
  • Journal Title: Crit. Rev. Biochem. Mol. Biol. 43 Pages: 277-283
  • Peer Reviewed
• [Journal Article] Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast. (2007)
  • Author(s): Matsuo, T., Otsubo, Y., Urano, J., Tamanoi, F., and Yamamoto, M.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 3154-3164
  • Peer Reviewed
• [Journal Article] Point mutations in TOR confer Rheb - independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells. (2007)
  • Author(s): Urano, J., Sato, T., Matsuo, T., Otsubo, Y., Yamamoto, M., and Tamanoi, F.
  • Journal Title: Proc. Natl. Acad. Sci. USA. 104 Pages: 3514-3519
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying the mitosis-meiosis decision. (2007)
  • Author(s): Harigaya, Y., and Yamamoto, M.
  • Journal Title: Chromosome Res. 15 Pages: 523-537
  • Peer Reviewed
• [Journal Article] Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes. (2007)
  • Author(s): Kohda, T. A., Tanaka, K., Konomi, M., Sato, M., Osumi, M., and Yamamoto, M.
  • Journal Title: Genes Cells 12 Pages: 155-170
  • Peer Reviewed
• [Journal Article] Selective elimination of messenger RNA prevents an incidence of untimely meiosis. (2006)
  • Author(s): Harigaya, Y., Tanaka, H., Yamanaka, S., Tanaka, K., Watanabe, Y., Tsutsumi, C., Chikashige, Y., Hiraoka, Y., Yamashita, A., and Yamamoto, M.
  • Journal Title: Nature 442 Pages: 45-50
  • Peer Reviewed
• [Journal Article] Fission yeast Numlp is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase. (2006)
  • Author(s): Yamashita, A., and Yamamoto, M.
  • Journal Title: Genetics 173 Pages: 1187-1196
  • Peer Reviewed
• [Journal Article] The Caenorhabditis elegans homologue of Deleted in Azoospermia is involved in the sperm/oocyte switch. (2006)
  • Author(s): Otori, M., Karashima, T., and Yamamoto, M.
  • Journal Title: Mol. Biol. Cell 17 Pages: 3147-3155
  • Peer Reviewed
• [Journal Article] C. elegans CPB-3 interacts with DAZ-1 and functions in multiple steps of germline development. (2006)
  • Author(s): Hasegawa, E., Karashima, T., Sumiyoshi, E. and Yamamoto, M.
  • Journal Title: Dev. Biol. 295 Pages: 689-699
  • Peer Reviewed
• [Journal Article] Fission yeast Meslp ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p. (2005)
  • Author(s): Izawa, D., Goto, M., Yamashita, A., Yamano, H., and Yamamoto, M.
  • Journal Title: Nature 434 Pages: 529-533
  • Peer Reviewed
• [Journal Article] Hrslp/Mcp6p on the meiotic SPB organizes astral microtubule arrays for oscillatory nuclear movement. (2005)
  • Author(s): Tanaka, K., Kohda, T., Yamashita, A., Nonaka, N., and Yamamoto, M.
  • Journal Title: Curr. Biol. 15 Pages: 1479-1486
  • Peer Reviewed
• [Journal Article] The roles of fission yeast Asel in mitotic cell division, meiotic nuclear oscillation and cytokinesis checkpoint signaling. (2005)
  • Author(s): Yamashita, A., Sato, M., Fujita, A., Yamamoto, M., and Toda, T.
  • Journal Title: Mol. Biol. Cell 16 Pages: 1378-1395
  • Peer Reviewed
• [Journal Article] C. elegans DAZ-1 is expressed in proliferating germ cells and directs proper nuclear organization and cytoplasmic core formation during oogenesis. (2005)
  • Author(s): Maruyama, R., Endo, S., Sugimoto, A., and Yamamoto, M.
  • Journal Title: Dev. Biol. 277 Pages: 142-154
  • Peer Reviewed
• [Journal Article] Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman spectroscopy (2005)
  • Author(s): Huang, Y. S., Karashima, T., Yamamoto, M., and Hamaguchi, H.
  • Journal Title: Biochemistry 44 Pages: 10009-10019
  • Peer Reviewed
• [Journal Article] The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. (2004)
  • Author(s): Niccoli, T., Yamashita, A., Nurse, P. and Yamamoto, M.
  • Journal Title: J. Cell Sci. 117 Pages: 5543-5556
  • Peer Reviewed
• [Presentation] Regulation of mitosismeiosis decision in fission yeast. (2008)
  • Author(s): Yamamoto, M.
  • Organizer: Asia-Pacific Regional S. pombe Meeting
  • Place of Presentation: Singapore
  • Year and Date: 20080725-17
• [Presentation] Molecular basis to remove meiotic mRNAs selectively in mitotic fission yeast cells. (2008)
  • Author(s): Yamamoto, M.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, New Hampshire, U.S.A.
  • Year and Date: 20080608-13
• [Presentation] Initiation of meiosis in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The 8th European Meiosis Meeting in Japan
  • Place of Presentation: Hayama, Japan
  • Year and Date: 20070913-18
• [Presentation] Selective elimination of mRNA as a novel paradigm for the regulation of meiosis. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The Fourth International Fission Yeast Meeting
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 20070611-16
• [Presentation] Selective elimination of messenger RNA as a switch between the mitotic and meiotic cell cycles in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: International Symposium "Ribosomes : From Structure to Gene Expression and Beyond"
  • Place of Presentation: Irvine, California, U.S.A.
  • Year and Date: 20070419-20
• [Presentation] Regulation of sexual development by TOR complexes in fission yeast. (2007)
  • Author(s): Yamamoto, M.
  • Organizer: The second International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20070325-28
• [Presentation] Selective elimination of mRNA provides a novel paradigm for the regulation of meiosis. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: RNA 2006 IZU
  • Place of Presentation: Izu, Japan
  • Year and Date: 20061203-07
• [Presentation] Molecular mechanism to eliminate meiosis-specific mRNA selectively in growing fission yeast cells. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New London, New Hampshire, U.S.A.
  • Year and Date: 20060611-16
• [Presentation] Selective elimination of meiotic mRNAs in mitotically growing fission yeast cells. (2006)
  • Author(s): Yamamoto, M.
  • Organizer: Cell Regulations in Division and Arrest Workshop
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20060306-09
• [Presentation] Identification of a novel RNA-binding protein that destabilizes meiosis-specific mRNAs during mitotic growth in fission yeast. (2005)
  • Author(s): Yamamoto, M.
  • Organizer: The 7th European Meiosis Meeting
  • Place of Presentation: Madrid, Spain
  • Year and Date: 20050913-18
• [Presentation] Novel factors regulating sexual differentiation in fission yeast. (2004)
  • Author(s): Yamamoto, M.
  • Organizer: The Third International Fission Yeast Meeting
  • Place of Presentation: San Diego, California, U.S.A.
  • Year and Date: 20040824-29
• [Book] 「体細胞分裂周期では減数分裂特異的mRNAが選択的に除去されている」蛋白質核酸酵素 (2007)
  • Author(s): 張ヶ谷有里子, 山下朗, 田仲加代子, 山本正幸
  • Total Pages: 1-10
  • Publisher: 共立出版
• [Book] 「減数分裂を制御するRNA-RNA結合蛋白質」蛋白質核酸酵素 (2006)
  • Author(s): 山下朗, 張ヶ谷有里子, 山本正幸
  • Total Pages: 2443-2449
  • Publisher: 共立出版
• [Book] 「減数分裂周期における細胞周期制御機構」実験医学 (2005)
  • Author(s): 伊澤大介, 山本正幸
  • Total Pages: 1404-1410
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/