Elucidating mechanisms of biological molecular machines by single-molecule physiology

2008 Fiscal Year Final Research Report

• Project/Area Number: 16002013
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Waseda University (2005-2008); National Institutes of Natural Sciences Okazaki Research Facilities (2004)
• Principal Investigator: KINOSITA Kazuhiko Waseda University, 理工学術院, 教授 (30124366)
• Project Period (FY): 2004 – 2008
• Keywords: 一分子観察 / 一分子操作 / 回転分子モーター / F1-ATPase / リニアー分子モーター / ミオシンV / 磁気ピンセット / 光ピンセット

Research Abstract

光学顕微鏡下の一分子生理学、とくに「一目で分かる」研究を標榜し、巨大目印による分子機械の動き・構造変化の直接可視化を通じて、動作機構の解明を試みた。回転分子モーターF1-ATPaseにおける化学反応→力学的仕事の共役スキームをほぼ完成させ、分子機械一般に通じ得る共役原理を提出した。一方、回転軸無しでも回転するという意外な発見をし、構造に基づく機能の説明は振出しに戻った。二本足のモーター、ミオシンの脚の動きの直接観察に成功し、ブラウン運動をうまく使って歩くことを証明した。

Research Products

• [Journal Article] A giant liposome for single-molecule observation of conforma-tional changes in membrane proteins (2009)
  • Author(s): Y. Onoue, T. Suzuki, M. Davidson, M. Karlsson, O. Orwar, M. Yoshida, and K. Kinosita Jr
  • Journal Title: Biochim. Biophys. Acta (Biomembranes) 1788 Pages: 1332-1340
  • Peer Reviewed
• [Journal Article] Neither helix in the coiled coil region of the axle of F1-ATPase plays a significant role in torque production (2008)
  • Author(s): M. D. Hossain, S. Furuike, Y. Maki, K. Adachi, T. Suzuki, A. Kohori, H. Itoh, M. Yoshida & K. Kinosita Jr
  • Journal Title: Biophys. J. 95 Pages: 4837-4844
  • Peer Reviewed
• [Journal Article] Temperature dependence of the rotation and hydrolysis activities of F1-ATPase (2008)
  • Author(s): S. Furuike, K. Adachi, N. Sakaki, R. Shimo-Kon, H. Itoh, E. Muneyuki, M. Yoshida & K. Kinosita Jr
  • Journal Title: Biophys. J. 95 Pages: 761-770
  • Peer Reviewed
• [Journal Article] Axle-less F1-ATPase rotates in the correct direction (2008)
  • Author(s): S. Furuike, M. D. Hossain, Y. Maki, K. Adachi, T. Suzuki, A. Kohori, H. Itoh, M. Yoshida & K. Kinosita Jr
  • Journal Title: Science 319 Pages: 955-958
  • Peer Reviewed
• [Journal Article] Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation (2007)
  • Author(s): K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida & K. Kinosita, Jr
  • Journal Title: Cell 130 Pages: 309-321
  • Peer Reviewed
• [Journal Article] Myosin V walks by lever action and Brownian motion (2007)
  • Author(s): K. Shiroguchi & K. Kinosita, Jr
  • Journal Title: Science 316 Pages: 1208-1212
  • Peer Reviewed
• [Journal Article] On the walking mechan- ism of linear molecular motors (2007)
  • Author(s): K. Kinosita, Jr., K. Shiroguchi, M. Y. Ali, K. Adachi, & H. Itoh
  • Journal Title: Adv. Exp. Med. Biol. 592 Pages: 369-384
• [Journal Article] The rotor tip inside a bearing of a thermophilic F1-ATPase is dispensable for torque generation (2006)
  • Author(s): M. D. Hossain, S. Furuike, Y. Maki, K. Adachi, M. Y. Ali, M. Huq, H. Itoh, M. Yoshida & K. Kinosita Jr
  • Journal Title: Biophys. J. 90 Pages: 4195-4203
  • Peer Reviewed
• [Journal Article] One rotary mechanism for F1-ATPase over ATP concentrations from millimolar down to nanomolar (2005)
  • Author(s): N. Sakaki, R. Shimo-Kon, K. Adachi, H. Itoh, S. Furuike, E. Muneyuki, M. Yoshida & K. Kinosita Jr
  • Journal Title: Biophys. J. 88 Pages: 2047-2056
  • Peer Reviewed
• [Journal Article] How two-foot molecular motors may walk (2005)
  • Author(s): K. Kinosita, Jr., M. Y. Ali, K. Adachi, K. Shiroguchi, & H. Itoh
  • Journal Title: Adv. Exp. Med. Biol. 565 Pages: 205-219
• [Journal Article] Unconstrained steps of myosin VI appear longest among known molecular motors (2004)
  • Author(s): M. Y. Ali, K. Homma, A. H. Iwane. K. Adachi, H. Itoh, K. Kinosita Jr. T. Yanagida, & M. Ikebe
  • Journal Title: Biophys. J. 86 Pages: 3804-3810
  • Peer Reviewed
• [Journal Article] Rotation of F1-ATPase : How an ATP-driven molecular machine may work (2004)
  • Author(s): K. Kinosita, Jr., K. Adachi, & H. Itoh
  • Journal Title: Annu. Rev. Biophys. Biomol. Struct. 33 Pages: 245-268
  • Peer Reviewed
• [Presentation] "How does F1-ATPase rotate? "Nobel Symposium on single molecule spectroscopy in chemistry, physics and biology (2008)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited symposium lecture
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20080601-06
• [Presentation] "Single-molecule physiology of protein machines" European Biophysics Congress (2007)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited plenary lecture
  • Place of Presentation: London, England
  • Year and Date: 20070714-18
• [Presentation] "Single-molecule physiology under an optical microscope" XXIII Biennial of the Society of Microscopy of Spain (2007)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited plenary lecture
  • Place of Presentation: Bilbao, Spain
  • Year and Date: 20070703-06
• [Presentation] "Single-molecule physiology of protein motors" Nano Tech Insight (2007)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited plenary lecture
  • Place of Presentation: Luxor, Egypt
  • Year and Date: 20070310-17
• [Presentation] "Probing Nature's Nanoscale Machines with Micro- scale Probes" Biophysical Society 50th annual meeting (2006)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited lecture as the 2006 National Lecturer
  • Place of Presentation: Salt Lake City, Utah, USA
  • Year and Date: 20060218-22
• [Presentation] "F1-ATPase : A molecular trans- ducer of chemical and mechanical energies" Nobel Symposium 132 : Energy in Cosmos, Molecules and Life (2005)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited symposium lecture
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20050618-22
• [Presentation] "F1-ATPase : a rotary motor / generator" Nobel Symposium 131 : Controlled Nanoscale Motion in Biological and Artificial Systems (2005)
  • Author(s): Kazuhiko Kinosita, Jr
  • Organizer: invited symposium lecture
  • Place of Presentation: Backaskog, Sweden
  • Year and Date: 20050613-17
• [Remarks] 研究室ホームページ(業績他)
  • URL: http://www.k2.phys.waseda.ac.jp/