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2005 Fiscal Year Final Research Report Summary

Biological studies on prions

Research Project

Project/Area Number 16017281
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagasaki University

Principal Investigator

KATAMINE Shigeru  Nagasaki University, Graduate School of Biomedical Sciences, Professa, 大学院・医歯薬学総合研究科, 教授 (40161062)

Project Period (FY) 2004 – 2005
Keywordsprion diseases / prion strain / prion protein / variant / structural conversion / cell culture
Research Abstract

Pathogenic agents of prion diseases, namely prions, have diverse strains each of which results in a characteristic clinical course and pathology in the inoculated mice. A previous study suggested that the mice infected with some prion strains were resistant against subsequent infection with other strains. In order to clarify the mechanisms, we examined whether the interference between prion strains were reproduced in the cell culture models. The mouse neuronal cells, GT1-7, persistently infected with a scrapie-derived mouse-adapted Chandler strain were permissive, but those with 22L strain were resistant against superinfection with a CJD-derived mouse-adapted strain, Fukuoka-1, indicating that 22L interferes Fukuoka-1 in neuronal cells without involvements of immune cells, Moreover, we demonstrated that an attenuated CJD strain, SY, successfully interfered subsequent challenges with virulent strains, Chandler, 22L, and Fukuoka-1. The findings have opened novel prophylactic and therapeutic strategies by the interference.

  • Research Products

    (22 results)

All 2007 2006 2005

All Journal Article (20 results) Book (2 results)

  • [Journal Article] Newly established in vitro system with fluorescent proteins shows that abnormal expression of downstream prion protein-like protein in mice is probably due to functional disconnection between splicing and 3' formation of prion protein pre-mRNA.2007

    • Author(s)
      Yoshikawa D et al.
    • Journal Title

      Gene 386(1-2)

      Pages: 139-146

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunization with recombinant bovine but not mouse prion protein delays the onset of disease in mice inoculated with a mouse-adapted prion.2007

    • Author(s)
      Ishibashi D et al.
    • Journal Title

      Vaccine 25(6)

      Pages: 985-992

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice.2007

    • Author(s)
      Miyazawa K et al.
    • Journal Title

      Histochem Cell Biol 127(3)

      Pages: 291-301

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Newly established in vitro system with fluorescent proteins shows that 3 abnormal expression of downstream prion protein -like protein in mice is probably due to functional disconnection between splicing and 3' formation of prion protein pre-mRNA2007

    • Author(s)
      Yoshikawa D, Kopacek J, Yamaguchi N, Ishibashi D, Yamanaka H, Yamaguchi Y, Katamine S, Sakaguchi S
    • Journal Title

      Gene 386

      Pages: 139-146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunization with recombinant bovine but not mouse prion protein delays the onset of disease in mice inoculated with a mouse-adapted prion.2007

    • Author(s)
      Ishibashi D, Yamanaka H, Yamaguchi N, Yoshikawa D, Nakamura R, Okimura N, Yamaguchi Y, Shigematsu K, Katamine S, Sakaguchi S
    • Journal Title

      Vaccine 25 (6)

      Pages: 985-992

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice.2007

    • Author(s)
      Miyazawa K, Kanaya T, Tanaka S, Takakura I, Watanabe K, Ohwada S, Kitazawa H, Rose MT, Sakaguchi S, K, Ohwada S, Kitazawa H, Rose MT, Sakaguchi S, Katamine S, Yamaguchi T, Aso H
    • Journal Title

      Flistochem Cell Biol. 127 (3)

      Pages: 291-301

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat-labile enterotoxin.2006

    • Author(s)
      Yamanaka H et al.
    • Journal Title

      Vaccine 24(15)

      Pages: 2815-2823

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] プリオン株をめぐる謎「特集・プリオン病とBSE」2006

    • Author(s)
      片峰 茂
    • Journal Title

      化学療法の領域 22巻1号

      Pages: 23-28

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] プリオン株をめぐる謎「特集・プリオン病とBSE」2006

    • Author(s)
      西田 教行
    • Journal Title

      化学療法の領域 22巻1号

      Pages: 71-80

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prion strain-dependent differences in conversion of mutant prion proteins in cell culture.2006

    • Author(s)
      Atarashi R et al.
    • Journal Title

      J Virol 80(16)

      Pages: 7854-7862

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Styrylbenzoazole derivatives for imaging of prion plaques and treatment of transmissible spongiform encephalopathies.2006

    • Author(s)
      Ishikawa K et al.
    • Journal Title

      J Neurochem 99(1)

      Pages: 198-205

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] プリオン仮説(タンパク質単独犯説)は本当か。2006

    • Author(s)
      片峰 茂
    • Journal Title

      科学(岩波書店) 11月号

      Pages: 1122-1126

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Semi-classical quantization of protein dynamics : novel NMR relaxation formalism and its application to prion.2006

    • Author(s)
      Kuwata K et al.
    • Journal Title

      Splinger-Verlag Tokyo, PRIONS; Food and Drug Safety (Ed : T Kitamoto)

      Pages: 155-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prion strain-dependent differences in conversion of mutant prion proteins in cell culture.2006

    • Author(s)
      Atarashi R, Sim VL, Nishida N, Caughey B, Katamine S
    • Journal Title

      J Virol 80 (16)

      Pages: 7854-7862

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Styrylbenzoazole derivatives for imaging of prion plaques and treatment of transmissible spongiform encephalopathies.2006

    • Author(s)
      Ishikawa K et al.
    • Journal Title

      J Neurochem. 99(1)

      Pages: 198-205

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biological and biochemical characteristics of prion strains conserved in persistently infected cell cultures.2005

    • Author(s)
      Arima K et al.
    • Journal Title

      J. Virol 79(11)

      Pages: 7104-7112

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reciprocal interference between specific CJD and scrapie agents in neural cell cultures.2005

    • Author(s)
      Nishida N et al.
    • Journal Title

      Science 21(5747)

      Pages: 493-496

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Female-specific neuroprotection against transient brain ischemia observed in mice devoid of prion protein is abolished by ectopic expression of prion protein-like protein.2005

    • Author(s)
      Sakurai-Yamashita Y et al.
    • Journal Title

      Neuroscience 136(1)

      Pages: 281-287

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biological and biochemical characteristics of prion strains conserved in persistently infected cell cultures.2005

    • Author(s)
      Arima K et al.
    • Journal Title

      J. Virol 79 (11)

      Pages: 7104-7112

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Female-specific neuroprotection against transient brain ischemia observed in mice devoid of prion protein is abolished by ectopic expression of prion protein-like protein2005

    • Author(s)
      Sakurai-Yamashita Y et al.
    • Journal Title

      Neuroscience 136(1)

      Pages: 281-287

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Splinger-Verlag Tokyo, PRIONS; Food and Drug Safety(Ed : T Kitamoto)2006

    • Author(s)
      Kuwata K et al.
    • Total Pages
      155-170
    • Publisher
      Semi-classical quantization of protein dynamics : novel NMR relaxation formalism and its application to prion.
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 論理的創薬入門-構造生物学に基づくアプローチ2006

    • Author(s)
      桑田一夫
    • Total Pages
      276
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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