2005 Fiscal Year Final Research Report Summary
Biological studies on prions
| Project/Area Number |
16017281
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagasaki University |
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Principal Investigator |
KATAMINE Shigeru Nagasaki University, Graduate School of Biomedical Sciences, Professa, 大学院・医歯薬学総合研究科, 教授 (40161062)
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| Project Period (FY) |
2004 – 2005
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| Keywords | prion diseases / prion strain / prion protein / variant / structural conversion / cell culture |
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| Research Abstract |
Pathogenic agents of prion diseases, namely prions, have diverse strains each of which results in a characteristic clinical course and pathology in the inoculated mice. A previous study suggested that the mice infected with some prion strains were resistant against subsequent infection with other strains. In order to clarify the mechanisms, we examined whether the interference between prion strains were reproduced in the cell culture models. The mouse neuronal cells, GT1-7, persistently infected with a scrapie-derived mouse-adapted Chandler strain were permissive, but those with 22L strain were resistant against superinfection with a CJD-derived mouse-adapted strain, Fukuoka-1, indicating that 22L interferes Fukuoka-1 in neuronal cells without involvements of immune cells, Moreover, we demonstrated that an attenuated CJD strain, SY, successfully interfered subsequent challenges with virulent strains, Chandler, 22L, and Fukuoka-1. The findings have opened novel prophylactic and therapeutic strategies by the interference.
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[Journal Article] Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice.2007
Author(s)
Miyazawa K, Kanaya T, Tanaka S, Takakura I, Watanabe K, Ohwada S, Kitazawa H, Rose MT, Sakaguchi S, K, Ohwada S, Kitazawa H, Rose MT, Sakaguchi S, Katamine S, Yamaguchi T, Aso H
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Journal Title
Flistochem Cell Biol. 127 (3)
Pages: 291-301
Description
「研究成果報告書概要(欧文)」より
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