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2005 Fiscal Year Final Research Report Summary

Biosyrdiesis of iron-sulfur proteins in Enlanoeba histotyrica

Research Project

Project/Area Number 16017302
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionGunma University (2005)
Kyoto Pharmaceutical University (2004)

Principal Investigator

NOZAKI Tomoyoshi  Gunma Univer, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (60198588)

Co-Investigator(Kenkyū-buntansha) ALI Vahab  Gunma University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20400757)
TAKAHASHI Yasuhiro  Osaka University, Graduate School of Science, Assistant Professor, 大学院・理学系研究科, 講師 (10154874)
Project Period (FY) 2004 – 2005
KeywordsEntamoeba histolytica / Parasitism / Electron transport / Iron sulfur cluster / Antioxidation / Enemy metabolism
Research Abstract

Iron clusters are well conserved among all living organisms, and play a pivotal role in respiration, electron transport, redox, and nitrogen tetion. In this study, we aimed at molecular characterization of iron sulfur biosynthesis in Entamoeba histolytica, and understanding of its role in parasitism and pathogenesis. We also aimed at obtaining a Mole view of cysteine and serine biosynthesis in the parasite. Iron-sulfur biosynthesis is known to be catalyzed by one or more of three independent pathways : NIF, ISC, and SUF, depending upon organisms. E histolytica exclusively possesses components for the NIF pathway, namely the catalytic component NifS and the scaffold component NifU. The presence of the NIF system in eukaryotes is unprecedented. We showed that the amebic NIF system is capable of all kinds of iron sulfur dusters, functionally replace the function of ISC and SUF in a heterologous organism, and function only under anaerobic conditions. We also showed the NIF system is localized to the cytosol, which is in good contrast to ISC and SUF, localized to the mitochondria and chloroplast, respectively. We also showed that E. histolytica possesses two pathways for serine metabolism, namely phosphorylated and non-phosphorylated pathways, which seems to be involved in biosynthesis aid gluconeogenesis, respectively. The presence of both the serine pathways is unique to this organism among parasitic protists. The present study should help our understanding of physiological importance of iron duster biosynthesis and serine/cysteine metaboism in parasitic organisms.

  • Research Products

    (12 results)

All 2005 2004 2003 Other

All Journal Article (12 results)

  • [Journal Article] Sulfur-containing amino acid metabolism in parasitic protozoa.2005

    • Author(s)
      Nozaki, T., Ali, V., Tokoro, M.
    • Journal Title

      Adv. Parasitol. 60

      Pages: 1-99

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biochemical and functional characterization of phosphoserine aminotransferase from Ent ameba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways.2005

    • Author(s)
      Ali, V., Nozaki, T.
    • Journal Title

      Mol. Biochem. Parasitol. 145

      Pages: 71-83

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The genome of the protist parasite Entamoeba histolytica.2005

    • Author(s)
      Loftus, B., et al.
    • Journal Title

      Nature 433

      Pages: 865-868

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An intestinal parasitic protist Entamoeba histolytica possesses a non-redundant NIF-like system for iron-sulfur cluster assembly under anaerobic conditions.2004

    • Author(s)
      Ali, V., Shigeta, Y., Tokumoto, U., Takahashi, Y., Nozaki, T.
    • Journal Title

      J. Biol. Chem. 179, 16863-16874. 279

      Pages: 16863-16874

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] (2004) Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica : a unique enteric protozoan parasite that possesses both phosphorylated and non-phosphorylated serine metabolic pathways.2004

    • Author(s)
      Ali, V., Shigeta, Y., Hashimoto, T., Nozaki, T.
    • Journal Title

      Eur. J. Biochem. 271, 2670-2681. 271

      Pages: 2670-2681

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular and structural characterization of NADPH-dependent D-glycerate dehydrogenase from the enteric parasitic protist Entamoeba histolytica.2003

    • Author(s)
      Ali, V., Shigeta, Y., Nozaki, T.
    • Journal Title

      Biochem. J. 375 : 729-736 375

      Pages: 729-736

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] (2005) Sulfur-containing amino acid metabolism in parasitic protozoa.

    • Author(s)
      Nozaki, T., AN, V., Tokoro, M.
    • Journal Title

      (Review) Adv. Parasitol. 60

      Pages: 1-100

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2006) Biochemical and functional characterization of phosphoserine aminotransferase from Entameba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways.

    • Author(s)
      Ali, V., Nozaki.T.
    • Journal Title

      Mol. Biochem. Parasitol. 145

      Pages: 71-83

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2005). The genome of the protist parasite Entamoeba histolytica.

    • Author(s)
      Loftus, B., Anderson, I., Davies, R., Alismark, U.C.M., Samuelson, J., Amedeo, P., Roncaglia, P., Berriman, M., Hirt, R.P., Mann, B.J., Nozaki, T., Suh, B., Pop, M., Duchene, M., Ackers, J., Tannich, E., Leippe, M., Hofer, M., Bruchhaus, I., Willhoeft, U., Bhattacharya, A., Chillingworth, TV, Churcher, C., Hance, Z., Harris, B., Harris, d., Jagels, K., Moule, S., Mungall, K., Ormond, D., Squares, R., Whitehead, S., Guillen, N., Gilchrist, C., Stroup, S.E., Bhattacharya, S., Lohia, A., Foster, P.G., Sicheritz-Ponten, T., Weber, C., Singh, U., Mukherjee, C., Petri, W.A.J., Clark, C.G., Embley, T.M., Barrell, B., Fraser, C.M., Hall, N.
    • Journal Title

      Nature 433

      Pages: 865-868

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2004) An intestinal parasitic protist, Entamoeba histolytica, possesses a non-redundant nitrogen fixation-like system for iron-sulfur cluster assembly under anaerobic conditions.

    • Author(s)
      Ali, V., Shigeta, Y., Tokumoto, U., Takahashi, Y., Nozaki, T.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 16863-16874

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2004) Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica : a unique enteric protozoan parasite that possesses both phosphorylated and non-phosphorylated serine metabolic pathways.

    • Author(s)
      Ali, V., Hashimoto, T., Shigeta, Y., Nozaki, T.
    • Journal Title

      Eur. J. Biochem. 271

      Pages: 2670-2681

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2003) Molecular and structural characterization of NADPH-dependent D-glycerate dehydrogenase from the enteric parasitic protist Entamoeba histolytica.

    • Author(s)
      Ali, V., Shigeta, Y., Nozaki, T.
    • Journal Title

      Biochem. J. 375

      Pages: 729-736

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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