2007 Fiscal Year Final Research Report Summary
Structures and functions of reaction spaces constructed using metalloprotiens
| Project/Area Number |
16074208
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Nagoya University |
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Principal Investigator |
WATANABE Yoshihito Nagoya University, Research Center for Materials Science, Professor (10201245)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Hiroshi Nagoya University, Graduate School of Science, Associate Professor (00283151)
UENO Takafumi Nagoya University, Graduate School of Science, Assistant Professor (70332179)
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| Project Period (FY) |
2004 – 2007
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| Keywords | Ferritin / Suzuki coupling / myoglobin / palladium / oraganometal complex / biocalalyst / metalloprotein / artificial protein |
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| Research Abstract |
Last year, we have specified the amino acid residues responsible for capturing Pd2+ ions on the interior surface of ap-ferritin by X-ray crystal structural studies. In addition, we have examined the residues which ligate to Au3+ ions and found that some of the Au ion binding sites in apo-ferritin are different from those of Pd ions with different coordi-nation structures. On the basis of these observation, we have prepared two type of metal nano-clusters, i.e., alloy and core/shell as shown in Schem 1. In the co-reduction, Au and Pd ions coexist in the apo-ferritin cage. The number of each ion in the cage is regulated by the affinity of each ions to its specific amino acid residues. Consequently, the alloy nano-particle was composed of 133 Au atoms and 165 Pd atoms ((AuPd)・apo-ferritin). On the other hand, we have successfully prepared Au-core/Pd-shell([Au] (Pd)) and Pd-core/Au-shell ([Pd] (Au)) types of nano-particles composed of 201 Au atoms/305 Pd atoms and 169 Au atoms/65Pd atoms, respectively. Among those apo-ferritin composites, [Au] (Pd) apo-ferritin shows the highest catalytic activity for the hydrogenation of olefins.
In a separate experiment, we have examined a possible incorporation of organometallic compounds such as [Pd(allyl)C1]2 and [Rh(norbornadiene)C1]2 in apo-ferritin. The crystal structure of Pd(allyl)・apo-ferritin clearly shows that the allyl group ligates to Pd ion in apo-ferritin.
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[Journal Article] Incorporation of a Phebox Rhodium Complex into apo-Myoglobin Affords a Stable Organometallic Protein Showing Unprecedented Arrangement of the Complex in the Cavity2007
Author(s)
Y. Satake, S. Abe, S. Okazaki, N. Ban, T. Hikage, T. Ueno, H. Nakajima, A. Suzuki, T. Yamane, H. Nishiyama, Y. Watanabe
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Journal Title
Organometallics 26
Pages: 4904-4908
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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