Regulatory mechanism of flagellar oscillation

2008 Fiscal Year Final Research Report

• Project/Area Number: 16083203
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SHINGYOJI Chikako The University of Tokyo, 大学院・理学系研究科, 准教授 (80125997)
• Co-Investigator(Kenkyū-buntansha): INABA Kazuo 筑波大学, 生命環境科学研究科, 教授 (80221779) ; YAGI Toshiki 京都大学, 大学院・理学研究科, 准教授 (40292833)
• Project Period (FY): 2004 – 2008
• Keywords: 分子モーター / ダイニン / 微小管 / 精子 / 鞭毛 / 滑り運動 / 振動 / 屈曲

Research Abstract

真核鞭毛の運動の基本は,鞭毛軸糸を構成する9本のダブレット微小管間に起こされる滑り運動であり,この滑りは,ダブレット上に並ぶダイニンが力を出すことによって起こされる.鞭毛内にある多種類のタンパク質は周辺制御因子としてダイニンのdynamic statesをモジュレートすることにより,屈曲形成にカップルしてダイニンの活性を制御し,その結果振動運動が時間空間的に巧みにコントロールされていると考えられる.本研究班では、ダイニンの特性をモジュレートする生理的条件および鞭毛の振動運動の発生に関わる要素を解明すると共に,ダイニンの活性制御に最も重要なダイニン結合制御因子と中心対微小管/スポーク系の分子構築を解明することにより,振動運動を生み出すダイニンのdynamic statesの制御機構を明らかにすることを目指した.その結果,ウニ精子鞭毛を用いて,振動運動機構の基礎となるダイニンによる滑りの切り替え機構とダイニンのヌクレオチド結合による活性調節の機構の解明,クラミドモナス鞭毛を用いて内腕ダイニンの機能特性の完全な理解,ホヤ精子プロテオーム解析からいくつもの制御因子の特性解析に成功し,振動運動機構の制御の理解に大きく貢献した.

Research Products

• [Journal Article] Bending-induced switching of dynein activity in elstase-treated axonemes of sea urchin sperm-Roles of Ca2+ and ADP. (2009)
  • Author(s): Hayashi, S., *Shingyoji, C.
  • Journal Title: Cell Motil. Cytoskel. 66 Pages: 292-301
  • Peer Reviewed
• [Journal Article] A novel neuronal calcium sensor family protein, calaxin, is a potential Ca2+-dependent regulator for the outer arm dynein of metazoan cilia and flagella (2009)
  • Author(s): Mizuno, K., Padma, P., Konno, A., Satouh, Y., Ogawa, K., *Inaba, K
  • Journal Title: Biol. Cell 101 Pages: 91-103
  • Peer Reviewed
• [Journal Article] Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella (2009)
  • Author(s): Yagi T, Uematsu K., Liu Z., Kamiya R
  • Journal Title: J. Cell Sci 122 Pages: 1306-1314
  • Peer Reviewed
• [Journal Article] Dynein pulls microtubules without rotating its stalk (2008)
  • Author(s): Ueno, H., Yasunaga, T., Shingyoji, C., *Hirose, K
  • Journal Title: Proc. Natl. Acad. Sci., USA 105 Pages: 19702-19707
  • Peer Reviewed
• [Journal Article] Mechanism of oscillation- bending-induced switching of dynein activity in elastase-treated axonemes of sea urchin sperm. (2008)
  • Author(s): Hayashi, S., *Shingyoji, C
  • Journal Title: J. Cell Sci 121 Pages: 2833-2843
  • Peer Reviewed
• [Journal Article] Molecular Characterization of Axonemal Proteins and Signaling Molecules Responsible for Chemoattractant-Induced Sperm Activation in Ciona intestinalis (2008)
  • Author(s): Hozumi A, Padma P, Toda T, Ide H, *Inaba K
  • Journal Title: Cell Motil. Cytoskel 65 Pages: 249-267
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins (2008)
  • Author(s): Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, *Takeda H
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Journal Article] Inhibition by ATP and activation by ADP in the regulation of flagellar movement in sea urchin sperm (2007)
  • Author(s): Yoshimura, A., Nakano, I., *Shingyoji, C
  • Journal Title: Cell Motil. Cytoskel 64 Pages: 777-793
  • Peer Reviewed
• [Journal Article] Induction of beating by imposed bending or mechanical pulse in demembranated, Motionless sea urchin sperm flagella at very low ATP concentrations (2007)
  • Author(s): Ishikawa, R., *Shingyoji, C
  • Journal Title: Cell Struct. Funct 32 Pages: 17-27
  • Peer Reviewed
• [Journal Article] The roles of noncatalytic ATP binding and ADP binding in the regulation of dynein motile activity in flagella (2007)
  • Author(s): Inoue, Y., *Shingyoji, C
  • Journal Title: Cell Motil Cytoskeleton 64 Pages: 690-704
  • Peer Reviewed
• [Journal Article] Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2 (2006)
  • Author(s): Hozumi A., Satouh Y., Makino Y., Toda T., Ide H., Ogawa K., King S.M., *Inaba, K
  • Journal Title: Cell Motil. Cytoskel 63 Pages: 591-603
  • Peer Reviewed
• [Journal Article] Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor (2005)
  • Author(s): Murata Y., Iwasaki H., Sasaki M., Inaba K., *Okamura Y
  • Journal Title: Nature 435 Pages: 1239-1243
  • Peer Reviewed
• [Journal Article] Molecular characterization of radial spoke subcomplex containing radial spoke protein 3 and heat shock protein 40 in sperm flagella of the ascidian Ciona intestinalis (2005)
  • Author(s): Satouh Y., Padma P., Toda T., Satoh N., Ide H., *Inaba K
  • Journal Title: Mol Biol Cell 16 Pages: 626-636
  • Peer Reviewed
• [Journal Article] An axonemal dynein particularly important for flagellar movement at high viscosity: Implication from a new Chlamydomonas mutant deficient in the dynein heavy chain gene dhc9 (2005)
  • Author(s): Yagi T., Minoura I., Fujiwara A., Saito R., Yasunaga T., Hirono M., *Kamiya R
  • Journal Title: J. Biol. Chem 280(50) Pages: 41412-20
  • Peer Reviewed
• [Journal Article] Effects of imposed bending on microtubule sliding in sperm flagella (2004)
  • Author(s): Morita, Y., *Shingyoji, C
  • Journal Title: Current Biol 14 Pages: 2113-2118
  • Peer Reviewed
• [Presentation] ダイニン・微小管複合体の構造学的研究(Structural analysis of outer arm dynein molecules bound to microtubules) (2008)
  • Author(s): 上野裕則、安永卓生、真行寺千佳子、広瀬恵子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡
  • Year and Date: 20081203-20081205
• [Presentation] クラミドモナス鞭毛の根元に局在する新規内腕ダイニンの同定 (2008)
  • Author(s): 八木俊樹、植松圭吾、劉中美、神谷律
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20081203-20081205
• [Presentation] Roles of motor protein in the regulation of melanophore responses in zebrafish (2008)
  • Author(s): Chikako Shingyoji
  • Organizer: The 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: University of Tokyo, Tokyo
  • Year and Date: 20081031-20081102
• [Presentation] Chikako Shingyoji, Keiko Hirose Structural analysis of outer arm dynein molecules bound to microtubules (2008)
  • Author(s): Hironori Ueno, Takuo Yasunaga Chikako Shingyoji, Keiko Hirose
  • Organizer: The 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: University of Tokyo, Tokyo
  • Year and Date: 20081031-20081102
• [Presentation] ウニ精子鞭毛外腕ダイニンによる微小管滑り運動に対するATPアナログの効果 (2008)
  • Author(s): 中野泉、真行寺千佳子
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] ウニ精子鞭毛における屈曲によるダイニン活性部位の切り替え誘導に対するADPの効果 (2008)
  • Author(s): 林周一、真行寺千佳子
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-20080907
• [Presentation] Comparative studies of cilia and flagella in the ascidian Ciona intestinalis (2008)
  • Author(s): Konno A, Inaba K
  • Organizer: 1st Int
  • Place of Presentation: Congress on Invertebrate Morphology, Copenhagen
  • Year and Date: 20080817-20080821
• [Presentation] Identificaiton of three minor inner-arm dynein heavy chains in Chlamydomonas flagella (2008)
  • Author(s): Yagi T, Uematsu K., Liu Z., Kamiya R
  • Organizer: EMBO Workshop on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Hyeres-les-Palmiers, Var, France
  • Year and Date: 20080527-20080601
• [Presentation] 新奇カルシウムセンサー「カラクシン」の鞭毛運動における機能解析 (2008)
  • Author(s): 水野克俊、志鷹裕司、榊原斉、大岩和弘、稲葉一男
  • Organizer: 第79回日本動物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-06
• [Presentation] Cancer/Testis antigen Sp17様タンパク質Ci-Sp17RPのホヤ精子活性化における機能解析 (2008)
  • Author(s): 保住暁子、笹倉靖徳、稲葉一男
  • Organizer: 第79回日本動物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-06
• [Presentation] カタユウレイボヤ精子運動活性化に関与する26kDaタンパク質の同定 (2008)
  • Author(s): 野村守、稲葉一男
  • Organizer: 第79回日本動物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-06
• [Presentation] ウニ精子鞭毛運動の制御におけるATP・ADPの役割 (2007)
  • Author(s): 吉村安寿弥, 真行寺千佳子
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学, 青森
  • Year and Date: 20070920-20070922
• [Presentation] ウニ精子鞭毛において屈曲により誘導されるダイニン活性部位の切り替え (2007)
  • Author(s): 林周一, 真行寺千佳子
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学, 青森
  • Year and Date: 20070920-20070922
• [Presentation] Roles of ATP, ADP and mechanical signals in the regulation of dynein activity in flagellar motility, International Conference on Molecular Mechanism of Intracellular Transport (2007)
  • Author(s): Yoshimura, A., Ishikawa, R., Inoue, Y., Nakano, I., Shingyoji, C
  • Organizer: The 56th Fujihara Seminar, The Roles of Kinesin and Dynein Superfamily Proteins
  • Place of Presentation: Grand Hotel New Oji in Tomakomai, Hokkaido, Japan
  • Year and Date: 20070823-20070826
• [Presentation] Regulation of dynein activity in flagellar motility: Roles of ATP, ADP and mechanical signals. 2007 FASEB Summer Reserch Conferences (2007)
  • Author(s): Chikako Shingyoji
  • Organizer: the Biology of Cilia and Flagella
  • Place of Presentation: Vermont Academy, Saxtons River, Vermont, USA
  • Year and Date: 20070804-20070809