Molecular mechnisms of dynein's force generation and regulations

2008 Fiscal Year Final Research Report

• Project/Area Number: 16083207
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: OIWA Kazuhiro National Institute of Information and Communications Technology, 未来ICT研究センター, センター長 (10211096)
• Co-Investigator(Renkei-kenkyūsha): IWAMOTO Hiroyuki 財団法人高緯度光科学研究センター, 利用研究促進準部門, 主幹研究員 (60176568)
• Research Collaborator: BURGESS Stan A. University of Leeds, Inst. Mol. Cellular Biol., Lecturer ; ISIKAWA Takashi ETH Zurich, Inst. Mol. Biol. Biophys., Groop Leader
• Project Period (FY): 2004 – 2008
• Keywords: 一分子計測(SMD) / 生物物理 / ナノマシン / ナノバイオ / 分子モーター

Research Abstract

本研究課題では、タンパク質モータ、軸糸ダイニンについて単一分子計測やアンサンブル機能計測、電子顕微鏡やX線溶液散乱による構造解析など、その構造と機能に関する研究を統一的に実施、ダイニンの運動発生機構とその制御機構の解明を目指した。生理的溶液中では伸展した構造をとるダイニンは、軸糸内においてコンパクトな構造となり隣接するダイニン分子の間に構造的連関を生じることを明らかにした。これは、個々の機能がアンサンブルとして高い次元の機能へと組織化して統合された鞭毛波を作り出すための構造的基盤である。機能面では運動特性の大きく異なる2種類のダイニンの協調的運動特性を明らかにして、鞭毛軸糸内でのアンサンブル特性創発の機能的基礎の一端を明らかにした。

Research Products

• [Journal Article] Cooperative three-step motions in catalytic subunits of F-1-ATPase correlate with 80 degress and 40 degrees substep rotations. (2008)
  • Author(s): Masaike, T., Koyama-Horibe, F., Oiwa K., Yoshida, M. & Nishizaka, T.
  • Journal Title: Nature Struct. Mol. Biol. 15 Pages: 1326-1333
  • Peer Reviewed
• [Journal Article] Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella. (2008)
  • Author(s): Bui, K. H., Sakakibara, H., Movassagh, T., Oiwa, K. & Ishikawa, T.
  • Journal Title: J. Cell Biol. 183 Pages: 923-932
  • Peer Reviewed
• [Journal Article] Molecular motors : Keep on moving (2008)
  • Author(s): Oiwa, K. and Kojima, H.
  • Journal Title: Nature Nanotech. 3 Pages: 531-532
• [Journal Article] Mechanical properties of inner-arm dynein-f (dynein I1) studied with in vitro motility assays. (2007)
  • Author(s): Kotani, N., Sakakibara, H., Burgess, S. A., Kojima, H., and Oiwa, K.
  • Journal Title: Biophys. J. 93 Pages: 886-894
  • Peer Reviewed
• [Journal Article] Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation (2007)
  • Author(s): Adachi, K., Oiwa, K., Nishizaka, T., Furuike, K., Noji, K., Itoh, H., Yoshida, M., and Kinosita, K. Jr.
  • Journal Title: Cell 130 Pages: 309-321
  • Peer Reviewed
• [Journal Article] The architectue of outer dynein arms in situ (2007)
  • Author(s): Ishikawa, T. Sakakibara, H., and Oiwa, K.
  • Journal Title: J. Mol. Biol. 368 Pages: 1249-1258
  • Peer Reviewed
• [Journal Article] Recent progress in dynein structure and mechanism. (2004)
  • Author(s): Oiwa, K., and Sakakibara, H.
  • Journal Title: Curr. Opin. Cell Biol. 17 Pages: 95-103
  • Peer Reviewed
• [Journal Article] The structure of dynein-c by negative stain electron microscopy (2004)
  • Author(s): Burgess, S. A., Sakakibara, H., Oiwa K., Kingh, P. J.
  • Journal Title: J. Struct. Biol 146 Pages: 205-215
  • Peer Reviewed
• [Journal Article] Chemomechanical coupling in F1-ATPase revealed by simulataneous observation of nucleotide kinetics and rotation (2004)
  • Author(s): Nishizaka, T., Oiwa, K., Noji, H., Kimura, S., Muneyuki, E., Yoshida, M., and Kinosita, K. Jr.
  • Journal Title: Nat. Struct. Mol. Biol. 11 Pages: 142-148
  • Peer Reviewed
• [Presentation] Mechanical and enzymatic properties of the heterodimeric axonemal dynein-f (2008)
  • Author(s): S. Toba
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京
  • Year and Date: 2008-11-01
• [Presentation] Conformational changes of dynein arms in situ studied using X-ray diffractions of Chlamydomonas flagellar axonemes (2008)
  • Author(s): K. Oiwa
  • Organizer: Gordon Research Conference Muscle & Molecular Motors
  • Place of Presentation: Boston
  • Year and Date: 2008-06-29
• [Presentation] Fluorescence Based Single Molecule Imaging and Nanometry on Axonemal Dyneins (2008)
  • Author(s): K. Oiwa
  • Organizer: 7th International Weber Symposium 2008
  • Place of Presentation: Hawai
  • Year and Date: 2008-06-10
• [Presentation] Force-generating mechanism of axonemal dyneins inspired by cryoelectron tomography and x-ray fiber diffraction analysis (2007)
  • Author(s): K. Oiwa
  • Organizer: The Abstbury Series, University of Leeds
  • Place of Presentation: Leeds
  • Year and Date: 2007-11-28
• [Presentation] The structure and mechanism of protein motors and their potential applications in the development of bio-nano-devices (2007)
  • Author(s): K. Oiwa
  • Organizer: D. B. Robinson Distinguished Speaker Lecture Series, University of Alberta
  • Place of Presentation: Edmonton, Canada
  • Year and Date: 2007-03-01
• [Presentation] Force generation mechanism of axonemal dynein inspired by the structure of isolated and in situ dyneins of Chlamydomonas axonemes (2006)
  • Author(s): K. Oiwa
  • Organizer: 2006 Biophysical Society Discussions, Molecular Motors : Point Counterpoint
  • Place of Presentation: Asilomar
  • Year and Date: 2006-10-20
• [Presentation] Force-generation mechanism of dynein of inspired by structures of isolated and in situ dyneins of Chlamydomonas (2006)
  • Author(s): K. Oiwa
  • Organizer: International Symposium on Bio-nanosystems
  • Place of Presentation: 仙台
  • Year and Date: 2006-09-03
• [Presentation] Structural studies on axonemal dynein-arms in chlamydomonas flagella by electron tomography (2005)
  • Author(s): T. Ishikawa
  • Organizer: International Workshop Dynein 2005, Molecular Mechanisms of Axonemal and Cytoplasmic Dyneins
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2005-10-31
• [Presentation] Raveling axonemal dyneins with single molecule and x-ray diffraction techniques (2005)
  • Author(s): K. Oiwa
  • Organizer: Motors & Cytoskeleton Conference
  • Place of Presentation: Hamburug-Blankenese
  • Year and Date: 2005-09-25
• [Presentation] Force generation mechanism of axonemal dyneins (2005)
  • Author(s): K. Oiwa
  • Organizer: Joint 61st Harden Conference/EMBO Workshop-Molecularmotors : structure and function
  • Place of Presentation: Cambridge
  • Year and Date: 2005-09-18
• [Presentation] Molecular mechanisms of AAA-proteins, FtsH and dynein studied (TIRF) microscopy (2005)
  • Author(s): K. Oiwa
  • Organizer: 6th International Weber Smposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine
  • Place of Presentation: Hawai
  • Year and Date: 2005-07-23
• [Book] Force-generating mechanisms of dynein revealed through single molecule studies. In single Molecule Biology (2009)
  • Author(s): Oiwa K. & Kojima, H.
  • Total Pages: 61-104
  • Publisher: Elsevier Academic Press, London.
• [Book] How linear motor proteins work, In Lecture Notes in Physics, Controlled Nanoscale Motion. H. Linke and A. Mansson, editors. (2007)
  • Author(s): Oiwa, K. & Manstein, D. J.
  • Total Pages: 41-63
  • Publisher: Springer/Berlin, Heidelberg, New York
• [Remarks] ホームページ等
  • URL: http://www2.nict.go.jp/w/w103/2131102/index.html
• [Patent(Industrial Property Rights)] 繊維状タンパク質の誘導素子及びその製造方法 (2006)
  • Inventor(s): 大岩和弘, 鈴木仁, 米谷玲皇, 松井真二
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Industrial Property Number: 特開, 2006-169120
  • Filing Date: 2006-06-29
• [Patent(Industrial Property Rights)] タンパク質モータの機能に基づく信号変換素子 (2006)
  • Inventor(s): 大岩和弘
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Industrial Property Number: 特許, 3878992
  • Acquisition Date: 2006-11-17
• [Patent(Industrial Property Rights)] 生理活性を持つ環状ヌクレオチド誘導体及びその製造方法 (2004)
  • Inventor(s): 大岩和弘
  • Industrial Property Rights Holder: (独)情報通信研究機構
  • Industrial Property Number: 特許, 3598366
  • Acquisition Date: 2004-09-24