細胞核ダイナミクス

2008 Fiscal Year Annual Research Report

• Project/Area Number: 16084101
• Research Institution: Osaka University
• Principal Investigator: 米田 悦啓 Osaka University, 生命機能研究科, 教授 (80191667)
• Keywords: 細胞核 / 分子イメージング / ゲノム / 細胞核内構造体 / 可視化技術 / 核-細胞質間輸送 / 染色体 / クロマチン

Research Abstract

本研究領域は、真核細胞の遺伝情報を保持し、発現させる基本構造体である細胞核を研究対象とし、その機能を構造構築のダイナミクスに立脚して統合的に理解することを目的としている。このため、本総括班では、領域内での研究の有機的な融合のため、定期的に開催する班会議を最も重要視し、班会議での発表、討論に基づいて、内部評価を厳密に行なうとともに、活発な意見交換、情報交換を促すことにより、各研究者が相互に影響を及ぼし合い、学際的な相互交流が進展することを目指している。この目的に沿って、本年度は、第5回領域班会議を2008年10月27日〜29日の3日間、京都ガーデンパレスで開催した。同会議では、計画班・公募班を問わず、班員すべてが研究成果や今後の計画を発表し、他の班員、総括班評価担当委員からの評価を受けた。同時に、前日の10月26日に、第8回総括班会議を開催し、主として本研究領域の活動をどのように総括し、次のステップにつなげていくかについて話し合った。一方、本特定領域のキーワードの1つである「可視化」技術は、日進月歩で開発が進んでおり、それらの技術を用いたデータが蓄積してきているため、昨年度から、本研究領域独自のデータベース作成に着手したが、本年度はさらなるバージョンアップを図った。また、ホームページを随時更新するとともに、2009年3月にニュースレターVol.5を刊行する予定であり、班員間の情報交換に努めるとともに、本研究領域の総括を行う予定である。また、2008年1月に姫路で開催された、第26回染色体ワークショップを後援した。

Research Products

• [Journal Article] Infrared laser-mediated gene_inductlon in targeted single cells in vivo (2009)
  • Author(s): Kamei, Y., et.al.
  • Journal Title: Nature Methods 6 Pages: 79-81
  • Peer Reviewed
• [Journal Article] mDia2 shuttles between the nucleus and the cytoplasm through the Importin-α/β- and CRMl-mediated nuclear transport, mechanism (2009)
  • Author(s): Miki, T., et.al.
  • Journal Title: J. Biol. Chem. 284 Pages: 99-104
  • Peer Reviewed
• [Journal Article] Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA (2009)
  • Author(s): Katahira, I., et.al.
  • Journal Title: EMBO J (in press)
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies (2009)
  • Author(s): Fujimura, K., et.al.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research (in press)
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor E47 via heterodimerization (2009)
  • Author(s): Mehmood, R., et.al
  • Journal Title: Exp. Cell Res. (in press)
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): Katahira, J. and Yoneda, Y.
  • Journal Title: RNA Biol. (in press)
  • Peer Reviewed
• [Journal Article] The role of nuclear transport system in cell differentiation (2009)
  • Author(s): Yasuhara, N., et.al.
  • Journal Title: Seminars in Cell and Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] Generation and characterization of a monoclonal antibody against NPI-1, subfamily importin α (2008)
  • Author(s): Tachibana, T., et.al.
  • Journal Title: Hybridoma 27 Pages: 285-289
  • Peer Reviewed
• [Journal Article] Localized regulation of aminal RanGTPase controls retrograde injury signaling in, peripheral nerve (2008)
  • Author(s): Yudin, D., et.al.
  • Journal Title: Neuron 59 Pages: 241-252
  • Peer Reviewed
• [Journal Article] Individual binding pockets of importin β for FG-nucleoporins are differently regulated_by RanGTP (2008)
  • Author(s): Otsuka, S., et.al.
  • Journal Title: Proc. Natl. Acad. Sel. USA 105 Pages: 16101-16106
  • Peer Reviewed
• [Presentation] C2C12細胞の筋分化における核膜孔複合体リモデリング (2009)
  • Author(s): Asally, M, Yasuda, Y, Oka, M, Otsuka, S, Yoshimura, SH, YoiLeda, Y
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 20090126-28
• [Presentation] 核蛋白質輸送と生命機能 (2008)
  • Author(s): 米田悦啓
  • Organizer: BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-12
• [Presentation] 精子細胞形成過程におけるimportin alphaの核内機能 (2008)
  • Author(s): 宮本洋一、安田善也、山城朋子、永井理博、米田悦啓、David Jans、 Kate Loveland
  • Organizer: BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-12
• [Presentation] Nuclear pore dynamics during skeletal myogenesis (2008)
  • Author(s): Asally, M., Yasuda,Y., Oka, M., Otsuka,S., Yoshimura, S. H., Yoneda,Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080917-21
• [Presentation] Nuclear import mechanism of neuroDl, E47 and their heterodimeric complex (2008)
  • Author(s): Mehmood, R., Yasuhara, N., Oe,S., Nagai,M., Yoneda, Y
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080917-21
• [Presentation] The role of Nup98 in a Crml-mediated nuclear export (2008)
  • Author(s): Oka,M., Asally, M., Yasuda, Y., Yoneda,Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080917-21
• [Presentation] Functional analysis of nuclear importin a accumulating in response to the stress (2008)
  • Author(s): Yasuda, Y., Miyamoto, Y., Yoneda, Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080917-21
• [Presentation] Nucleo-cytoplasmic transport and the assembly of stress granules (2008)
  • Author(s): Ken Fujimura, Jun Katahira, Fumi Kano, Yoshihiro Yoneda, Masayuki Murata
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080917-21
• [Presentation] Nuclear protein import and its significance to cell function (2008)
  • Author(s): Yoneda, Y.
  • Organizer: 2nd. Intarnational_SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] Regulation of importin aLphal expression (2008)
  • Author(s): Yasunao Kamikawa, Noriko Yasehura, Yoshihiro Yoaneda
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] A novel regulatory system to maintain ES cells through nuclear-cytoplasmic transport (2008)
  • Author(s): Yasuhara, N, Memood, R, Kamikawa, Y., Touma, K. and Yoneda, Y.
  • Organizer: 2nd Intetnational SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] Nuclear-cytoplasmic transport regulation of transcription factors (2008)
  • Author(s): Memood, R., Yoshikawa, K., Shibazaki, N., Kaneko, A., Yasuhara, N., Kondoh, H and Yoneda, Y.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] Nuclear import mechanism of Nanog (2008)
  • Author(s): Gibo, R., Okada, M., Yasuh ara. N. And Yoneda, Y.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] Selective localization of PCBP2 to P-bodies-An implication for functional diversity among P-bodies (2008)
  • Author(s): Ken Fujimura, Jun Katahira, Fumi Kano, Yoshihiro Yoneda, Masayuki, Marata
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N. Y. /USA
  • Year and Date: 20080903-07
• [Presentation] Nucleocytoplasmic transport of proteins and RNAs and its sig nificance.to cell function (2008)
  • Author(s): Yoneda, Y., Katahira, J., Sekimoto, T., Yasuhara, N., and Oka, M.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Functional differences between two isoforms of Npap60(Nup50)in nuclear impiort (2008)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M., Asally, M. and Yoneda, Y.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Nuclear accumulation of importin a suppresses DNA replication (2008)
  • Author(s): Mizuguchi, C., Yasuda, Y. and Yoneda, Y.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Analysis of potential roles of Nup98 FG repeat region in oncogenesis (2008)
  • Author(s): Masahiro Oka, Munehiro Asally, Yoshinari Yasuda, Yoshihiro Yoneda
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Iaitificdation of a cis-acting dendritic targeting element in the brain-derived neurotrophic factor mRNA (2008)
  • Author(s): Souichi Oe, Yoshihiro Yoneda
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Book] 分子生物学イラストレイテット第3版 (2009)
  • Author(s): 浅利宗弘、米田悦啓
  • Total Pages: 348
  • Publisher: 羊土社
• [Book] 標準細胞生物学 (2008)
  • Author(s): 片平じゅん、米田悦啓
  • Total Pages: 358
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/