Researches on intra-nuclear architecture and regulation of chromatinfunction

2008 Fiscal Year Final Research Report

• Project/Area Number: 16084201
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: HARATA Masahiko Tohoku University, 大学院・農学研究科, 准教授 (70218642)
• Co-Investigator(Kenkyū-buntansha): TSUTSUI Ken 岡山大学, 自然生命科学研究支援センター, 教授 (70108158)
• Project Period (FY): 2004 – 2008
• Keywords: 細胞核 / クロマチン / アクチン関連タンパク質 / ゲノム機能 / 遺伝子発現

Research Abstract

真核生物ゲノムの機能制御には、クロマチンと細胞核が中心的な役割を果たしている。本研究では、細胞核に局在するアクチンファミリーであるアクチン関連タンパク質(actin-related protein ; Arp)に主に焦点を当て、細胞核の内部構造の分子構築がどのようにクロマチンやゲノムの機能に関与しているかを明らかにすることを目的として研究を進めた。その結果、クロマチンや細胞核の構築において、Arpが様々な分子機構で関与することを明らかにすることができた。

Research Products

• [Journal Article] Actin-related protein Arp6 influences H2AZ-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores (2010)
  • Author(s): Yoshida, T., Shimada, K., Oma, Y., Kalck, V., Akimura, K., Taddei, A., Iwahashi, H., Kugou, K., Ohta, K., Gasser, S.M., Harata, M
  • Journal Title: PLoS Genet (in press)
  • Peer Reviewed
• [Journal Article] Identification and characterization of two isoforms of the vertebrate H2A. Z histone variant (2010)
  • Author(s): Matsuda, R., Hori, T., Kitamura, H., Takeuchi, K., Fukagawa, T., Harata, M
  • Journal Title: Nucleic Acids Res (in press)
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4 (2010)
  • Author(s): Kumeta, M., Yoshimura, S.H., Harata, M., Takeyasu, K
  • Journal Title: J. Cell Sci (in press)
  • Peer Reviewed
• [Journal Article] Involvement of actin-related protein Arp6 in organization of chromosomes (2010)
  • Author(s): Ohfuchi Maruyama, E., Harata, M
  • Journal Title: Adv. Chromosome Sc (in press)
• [Journal Article] The human actin-related protein hArp5: nucleo-cytoplasmic shuttling and involvement in DNA repair (2009)
  • Author(s): Kitayama, K., Kamo, M., Oma, Y., Matsuda, R., Uchida, T., Ikura, T., Tashiro, S., Ohyama, T., Winsor, B., Harata, M
  • Journal Title: Exp. Cell Res 315 Pages: 206-217
  • Peer Reviewed
• [Journal Article] Ino80 Chromatin Remodeling Complex Promotes Recovery of Stalled Replication Forks (2008)
  • Author(s): Shimada, K., Om, a Y., Schleker, T., Kugou, K., Ohta, K., Harata, M., *Gasser SM
  • Journal Title: Curr. Biol 18 Pages: 566-575
  • Peer Reviewed
• [Journal Article] The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment (2008)
  • Author(s): Aoyama, N., Oka, A., Kitayama, K., Kurumizaka, H., Harata, M
  • Journal Title: Exp. Cell Res 314 Pages: 859-868
  • Peer Reviewed
• [Journal Article] The nuclear actin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization (2008)
  • Author(s): Miloshev, J., Harata, M., Georgieva, M
  • Journal Title: J. Cell Biochem 104 Pages: 59-67
  • Peer Reviewed
• [Journal Article] Topoisomerase IIb activates a subset of neuronal genes that are repressed in AT-rich genomic environment (2008)
  • Author(s): Sano, K., Miyaji-Yamaguchi, M., Tsutsui, KM., Tsutsui, K
  • Journal Title: PLoS ONE 3(12) Pages: e4103(1-13)
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly (2007)
  • Author(s): Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki M
  • Journal Title: Nucleic Acids Res 35 Pages: 3109-3117
  • Peer Reviewed
• [Journal Article] The INO80 complex is required for damage-induced recombination (2007)
  • Author(s): Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M
  • Journal Title: Biochem. Biophys. Res. Commun 355 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Vertebrate Arp6, a novel nuclear actin-related protein, interacts with heterochromatin protein 1 (2006)
  • Author(s): Ohfuchi, E., Kato, M., Sasaki, M., Sugimoto, K., Oma, Y., Harata, M
  • Journal Title: Eur. J. Cell Biol 85 Pages: 411-421
  • Peer Reviewed
• [Journal Article] Assembly of staphylococcal leukocidin into a pore-forming oligomer on detergent-resistant membrane microdomains, lipid rafts, in human polymorphonuclear leukocytes (2006)
  • Author(s): Nishiyama, A., Kaneko, J., Harata, M., Kamio, Y
  • Journal Title: Biosci. Biotechnol. Biochem 70 Pages: 1300-1307
  • Peer Reviewed
• [Journal Article] Correlations between scaffold/matrix attachment region (S/MAR) binding activity and DNA duplex destabilization energy (2006)
  • Author(s): Bode, J., Winkelmann, S., Gotze, S., Spiker, S., Tsutsui, K., Bi, C., Prashanth, A.K., Benham, C
  • Journal Title: J. Mol. Biol 358 Pages: 597-613
  • Peer Reviewed
• [Journal Article] Expression dynamics and functional implications of DNA topoisomerase IIb in the brain (2006)
  • Author(s): Tsutsui, KM., Sano, K., Hosoya, O., Tsutsui, K
  • Journal Title: Anat. Sci. Int 81 Pages: 156-163
  • Peer Reviewed
• [Journal Article] Nulcear actin-related proteins involved in the organization of euchromatin and heterochromatin. DNA structure, chromatin, and gene expression (2006)
  • Author(s): Harata, M., Ohfuchi, E., Oma, Y
  • Journal Title: Transworld Research Network Inc(eds. Shimizu, M. and Kiyama, R. ) Pages: 181-112
• [Journal Article] Actin-related proteins involved in nuclear and chromatin dynamics (2006)
  • Author(s): Harata, M
  • Journal Title: Springer-Verlag Tokyo, Inc(Nuclear Dynamics: Approaches from Biochemistry, Molecular Cell Biology, and Visual Biology (eds. Nagata, S. and Takeyasu, K. )) Pages: 239-248
• [Journal Article] 核の構造とその分子基盤蛋白質核酸酵素 (2006)
  • Author(s): 原田昌彦、北山久美子、尾間由佳子
  • Journal Title: 51 Pages: 591-599
• [Journal Article] クロマチン蛋白質核酸酵素 (2006)
  • Author(s): 原田昌彦
  • Journal Title: 51 Pages: 1943-1949
• [Journal Article] The nuclear actin-related protein Act3p/Arp4p is involved in the dynamics of chromatin modulating complexes (2005)
  • Author(s): Sunada, R., Goerzer, I., Oma, Y., Yoshida, T., Suka, N., Wintersberger, U., Harata, M
  • Journal Title: Yeast 22 Pages: 753-768
  • Peer Reviewed
• [Journal Article] Large-scale identification and mapping of nuclear matrix-attachment regions in the distal imprinted domain of mouse chromosome 7 (2004)
  • Author(s): Purbowasito, W., Suda, C., Yokomine, T., Zuair, M., Sado, T., Tsutsui, K., Sasaki, H
  • Journal Title: DNA Res 11 Pages: 391-407
  • Peer Reviewed
• [Presentation] クロマチンリモデリングおよびヒストンバリアントによるゲノム機能制御 (2009)
  • Author(s): 原田昌彦
  • Organizer: 平成21年度国立遺伝学研究所研究会-クロマチン・染色体の分子構築と機能制御
  • Place of Presentation: 三島
  • Year and Date: 20091112-20091113
• [Presentation] Actin-related proteins functional organization of chromatin (2009)
  • Author(s): Yoshida T, Shimada K, Oma Y, Kugou K, Ohta K, Hori T, Fukagawa T, Gasser S, Harata M
  • Organizer: Switzerland-Japan Joint Meeting on the Molecular Mechanisms regulating "Chromosome Dynamics and Genome Stability"
  • Place of Presentation: Villars, Switzerland
  • Year and Date: 20090514-20090516
• [Presentation] Roles of actin-related proteins in nuclear architecture and genome function (2009)
  • Author(s): Masahiko Harata
  • Organizer: 第32回日本分子生物学会年会シンポジウム「細胞核アーキテクチャーに基づくゲノム機能制御:そのコンセプトの最新像」
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 脊椎動物のアクチン関連タンパク質による染色体・細胞核機能構造の形成 (2009)
  • Author(s): 原田昌彦
  • Organizer: 日本遺伝学会第81回大会ワークショップ「核ダイナミクス研究の新展開」
  • Place of Presentation: 松本
  • Year and Date: 2009-09-17
• [Presentation] アクチン関連タンパク質Arp6の核膜との機能的相互作用およびクロマチン核内配置への関与 (2009)
  • Author(s): 吉田貴人、島田健士、尾間由佳子、秋村和美、岩橋均、久郷和人、太田邦史、Susan Gasser、原田昌彦
  • Organizer: 第61回日本細胞生物学会大会ミニシンポジウム-細胞骨格、核膜と核機能の連係
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] Involvement of the actin-related protein Arp6 in spatial organization of radial chromosome territories (2008)
  • Author(s): Harata, M
  • Organizer: The 3rd Asian Chromosome Colloquium
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20081201-20081204
• [Presentation] Multiple functions of actin-related proteins in chromatin and nuclear organization (2008)
  • Author(s): Harata, M
  • Organizer: Nuclear Signaling Network -Epigenetics and Development-
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2008-12-12
• [Presentation] ゲノム機能制御におけるクロマチンリモデリング複合体の多様な機能 (2008)
  • Author(s): 原田昌彦
  • Organizer: 国立遺伝学研究所研究会 クロマチン研究会-細胞核・染色体・クロマチンの機能構造構築と動態-
  • Place of Presentation: 三島
  • Year and Date: 2008-10-22
• [Presentation] コンフォーカルレーザー顕微鏡などによるバイオイメージングに基づく細胞機能解析 (2008)
  • Author(s): 原田昌彦
  • Organizer: 第62回日本栄養・食糧学会大会(女子栄養大学)イブニングセミナー「若手研究者のための栄養・食糧学技術セミナー」
  • Place of Presentation: 板戸
  • Year and Date: 2008-05-03
• [Presentation] アクチンファミリーと細胞核・クロマチンの機能構造文部科学省科学研究費助成金「特定領域究」2領域合同 (2008)
  • Author(s): 原田昌彦
  • Organizer: 公開シンポジウム「クロマチンシグナリングの分子機構」
  • Place of Presentation: 東京
  • Year and Date: 2008-01-08
• [Presentation] クロマチンの核内配置とアクチン関連タンパク質:クロマチン工学への応用 (2007)
  • Author(s): 吉田貴人、島田健士、秋村和美、久郷和人、太田邦史、岩橋均、Susan Gasser、原田昌彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会ワークショップ「クロマチン工学の開拓」
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] アクチン関連タンパク質による核構築とゲノム機能制御 (2007)
  • Author(s): 原田昌彦
  • Organizer: 平成19年度国立遺伝学研究所研究会「クロマチン研究会-ゲノム・細胞核から個体発生まで-」
  • Place of Presentation: 三島
  • Year and Date: 20071025-20071026
• [Presentation] Actin-related proteins involved in chromatin and nuclear organization (2007)
  • Author(s): Yoshida T, Shimada K, Kugo K, Ohta K, Gasser S, Harata M
  • Organizer: 第59回日本細胞生物学会シンポジウム「細胞核構造構築のダイナミクス」
  • Place of Presentation: 博多
  • Year and Date: 2007-05-28
• [Presentation] 細胞核のアクチンファミリーによるゲノムダイナミクス制御 (2007)
  • Author(s): 原田昌彦、吉田貴人、大渕恵理、尾間由佳子、久郷和人、太田邦史、Susan Gasser、島田健士
  • Organizer: 日本農芸化学会シンポジウム「ゲノムダイナミクスの理解とその応用展開」
  • Place of Presentation: 東京
  • Year and Date: 2007-03-27
• [Presentation] Actin-related proteins involved in dynamics organization of chromatin and cell nucleus (2007)
  • Author(s): Harata, M
  • Organizer: International Symposium -Functional Organization of the nucleus-
  • Place of Presentation: Awaji, Hyogo, Japan
  • Year and Date: 2007-01-09
• [Presentation] 細胞核・クロマチンのダイナミクスとアクチン関連タンパク質Arp6 (2006)
  • Author(s): 原田昌彦
  • Organizer: 国立遺伝学研究所研究会「クロマチンダイナミクスとゲノム機能制御」
  • Place of Presentation: 三島
  • Year and Date: 2006-10-15
• [Presentation] ヒトhArp5とhIno80の細胞核・ゲノム機能への関与 (2005)
  • Author(s): 北山久美子、松田涼、原田昌彦
  • Organizer: 遺伝研研究会「クロマチンダイナミクスとゲノム機能制御」
  • Place of Presentation: 三島
  • Year and Date: 2005-10-07
• [Presentation] 細胞核内構造-クロマチン相互作用とゲノム機能制御 (2005)
  • Author(s): 原田昌彦
  • Organizer: 日本農芸化学会東北支部シンポジウム「クロマチンの化学-ゲノム機能の鍵を握るタンパク質複合体の驚異-」
  • Place of Presentation: 仙台
  • Year and Date: 2005-07-02
• [Presentation] 細胞核・染色体・クロマチンの機能構造とアクチン関連タンパク質 (2004)
  • Author(s): 原田昌彦
  • Organizer: 国立遺伝学研究所研究会「DNAの高次構造とクロマチンに印された情報の理解に向けて」
  • Place of Presentation: 三島
  • Year and Date: 2004-09-10
• [Book] 実験医学増刊号「細胞核-遺伝情報制御と疾患」 (2009)
  • Author(s): 平岡泰、原田昌彦、木村宏、田代聡
  • Total Pages: 233
  • Publisher: 羊土社
• [Book] 実験医学増刊号「細胞核-遺伝情報制御と疾患」(平岡泰、原田昌彦、木村宏、田代聡:編) (2009)
  • Author(s): 尾間由佳子、松田涼、北村大志、原田昌彦
  • Total Pages: 2752-2758
• [Book] DNA複製と遺伝子発現ベーシックマスター生化学(大山隆:監修) (2008)
  • Author(s): 原田昌彦、大山隆
  • Total Pages: 289-324
  • Publisher: オーム社
• [Book] ベーシックマスター分子生物学(東中川徹、大山隆、清水光弘:編) (2006)
  • Author(s): 原田昌彦
  • Total Pages: 196-219
  • Publisher: オーム社
• [Book] 細胞核の分子生物学(水野重樹:編) (2005)
  • Author(s): 原田昌彦
  • Total Pages: 34-58
  • Publisher: 朝倉書店