Molecular dynamics of nuclear pore complexes and regulation of nucleocytoplasmic protein transport

2008 Fiscal Year Final Research Report

• Project/Area Number: 16084204
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: YONEDA Yoshihiro Osaka University, 生命機能研究科, 教授 (80191667)
• Project Period (FY): 2004 – 2008
• Keywords: 細胞核 / 核タンパク質輸送 / 細胞分化 / 核膜孔複合体 / ES 細胞 / 筋芽細胞

Research Abstract

細胞核の重要な構造体である核膜孔複合体に焦点を当て、その分子動態が複雑な生命現象とどのように深く関連しているのかを解析した。その結果、筋芽細胞から筋細胞への分化の過程で、核膜孔複合体構成因子の1つNUP358の発現量が分化に応じて変動し、その変動が筋分化に必須であることがわかった。また、核輸送因子importin・サブタイプ発現のスイッチングが細胞の分化・未分化決定機構に重要な役割を果たすことを、マウスES細胞を用いて明らかにした。

Research Products

• [Journal Article] Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. (2009)
  • Author(s): Katahira, J., Inoue, H., Hurt, E. and Yoneda, Y.
  • Journal Title: EMBO J. 28 Pages: 556-567
  • Peer Reviewed
• [Journal Article] Localized regulation of axonal RanGTPase controls retrograde injury signaling in peripheral nerve. (2008)
  • Author(s): Yudin, D. ,Hanz, S., Yoo, S. avnilovitch, E., Willis, D., Gradus, T., Segal-Ruder, Y., Ben-Yaakov, K., Hieda, M., Yoneda, Y., Twiss, J. L. and Fainzilber, M
  • Journal Title: Neuron 59 Pages: 241-252
  • Peer Reviewed
• [Journal Article] Triggering neural differentiation of ES cells by subtype switching of importin-α (2007)
  • Author(s): Yasuhara, N., shibazaki, N., Tanaka, S., Nagai , M., Kamikawa, Y., Oe, S., Kamachi, Y., K ondoh, H. and Yoneda, Y.
  • Journal Title: Nature Cell Biol. 9 Pages: 72-79
  • Peer Reviewed
• [Journal Article] Arx1 functions as an unorthodox nuclear export receptor for the 60S pre-ribosomal subunit. (2007)
  • Author(s): Bradatsch, B., Katahira, J., Kowalinski, E., Bange, G., Yao, W. ,Sekimoto, T., Baumgrtel, V, Boese, G., Bassler, J., Wild, K. ,Peters, R., Yoneda, Y., Sinning, I. and Hurt, E.
  • Journal Title: Mol. Cell 27 Pages: 767-779
  • Peer Reviewed
• [Journal Article] PGC7/Stella as a protector against DNA demethylation in early embryogenesis. (2007)
  • Author(s): Nakamura, T., Arai, Y., Umehara, H., Masuhara, M., Kimura, T., Taniguchi, H., Sekimoto, T., Ikawa, M., Yoneda, Y., Okabe, M., Tanaka, S., Shiota, K. and Nakano, T.
  • Journal Title: Nature Cell Biol. 9 Pages: 64-71
  • Peer Reviewed
• [Journal Article] Exportin-5 orthologues are functionally divergent among species. (2006)
  • Author(s): Shibata, S., Sasaki, M., Miki, T., Shimamoto, A., Furuichi, Y., Katahira, J., and Yoneda, Y.
  • Journal Title: Nucl. Acids Res. 34 Pages: 4711-47211
• [Journal Article] Importin transports Ca^<2+>/calmodulin-dependent protein kinase type IV (CaMKIV) to the nucleus without utilizing importin (2005)
  • Author(s): Kotera, I., Sekimoto, T., Miyamoto, Y., Saiwaki, T., Nagoshi, E., Sakagami, H., Kondo, H. and Yoneda, Y.
  • Journal Title: EMBO J., 24 Pages: 942-951
  • Peer Reviewed
• [Journal Article] The 70 kDa heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors. (2005)
  • Author(s): Kose, S., Furuta, M. ,Koike, M., Yoneda, Y. and Imamoto, N.
  • Journal Title: J.Cell Biol. 171 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Cellular stresses induce the nuclear accumulation of importin α and cause a conventional nuclear import block. (2004)
  • Author(s): Miyamoto, Y., Saiwaki, T., Yamashita, J., Yasuda, Y. ,Kotera, I., Shibata, S., Shigeta, M., Hiraoka, Y., Haraguchi, T., and Yoneda, Y.
  • Journal Title: J.Cell Biol. 165 Pages: 617-623
  • Peer Reviewed
• [Journal Article] 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27^<Kip1>. (2004)
  • Author(s): Sekimoto, T., Fukumoto, M., and Yoneda, Y.
  • Journal Title: EMBO J. 23 Pages: 1934-1942
  • Peer Reviewed
• [Presentation] 核蛋白質輸送と生命機能 (2008)
  • Author(s): 米田悦啓
  • Organizer: BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-12
• [Presentation] Nuclear pore dynamics during skeletal myogenesis. (2008)
  • Author(s): Asally,M., Yasuda,Y., Oka,M.,Otsuka,S., Yoshimura,S.H., Yoneda,Y.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: N.Y./USA
  • Year and Date: 20080917-21
• [Presentation] Nuclear protein import and its significance to cell function (2008)
  • Author(s): Yoneda, Y.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫
  • Year and Date: 20080916-17
• [Presentation] Importin alpha has multiple functionsEMBO (2007)
  • Author(s): Yoneda, Y.
  • Organizer: Workshop on Mechanisms of Nucleocytoplasmic Transport
  • Place of Presentation: Italy
  • Year and Date: 20071027-31
• [Presentation] 核-細胞質間分子流通機構と生命機能の調節 (2007)
  • Author(s): 米田悦啓
  • Organizer: 第71 回日本生化学会中部支部会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-05-19
• [Book] 分子生物学イラストレイテッド第3版 (2009)
  • Author(s): 浅利宗弘、米田悦啓
  • Total Pages: 348
  • Publisher: 羊土社
• [Book] 標準細胞生物学 (2008)
  • Author(s): 片平じゅん、米田悦啓
  • Total Pages: 358
  • Publisher: 医学書院
• [Book] Nuclear Dynamics (2007)
  • Author(s): Saiwaki,T. and Yoneda,Y
  • Total Pages: 279
  • Publisher: Springer-Verlag Inc
• [Book] 細胞核の世界-ダイナミクスから病態まで (2007)
  • Author(s): 米田悦啓、他
  • Total Pages: 383
  • Publisher: 共立出版
• [Remarks] ホームページ等
  • URL: http://www.anat3.med.osaka-u.ac.jp/