2008 Fiscal Year Final Research Report
Induction mechanism of endoplasmic-reticulum-derived organelle
| Project/Area Number |
16085203
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
HARA-NISHIMURA Ikuko Kyoto University, 大学院・理学研究科, 教授 (00241232)
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| Project Period (FY) |
2004 – 2008
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| Keywords | シロイヌナズナ / ERボディ / 傷害応答 / 小胞体 / bグルコシダーゼ / PYK10 / NAI1 / NAI2 |
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| Research Abstract |
We identified a distinct type of ER-derived structure as a new organelle in Arabidopsis, which we have designated the ER body. The spindle-shaped, 5-10 μm long ER bodies are easily detected in Arabidopsis expressing ER-targeted GFP. ER bodies are uniformly distributed throughout the epidermis of cotyledons and hypocotyls in young seedlings. Interestingly, wounding or treatment with the wound hormone jasmonate induces the accumulation of ER bodies in adult leaves. This suggests that the ER body is involved in pest/pathogen resistance in Arabidopsis. The ER bodies in Arabidopsis seedlings accumulate PYK10 protein, which bears the ER retention signal KDEL. The Arabidopsis nai1 mutant lacks ER bodies. The NAI1 gene encodes a basic helix-loop-helix-type transcription factor. NAI1 regulates the expression of PYK10, JAL22, JAL23, JAL31, JAL33, PBP1/JAL30, GLL2 and GLL25. PYK10 forms a large complex with JALs and GLLs in cells that are disrupted. JALs and GLLs regulate the size of the PYK10 complex and may regulate its substrate specificity. As NAI1 deficiency causes the loss of ER bodies, NAI1 may regulate unknown factors that are responsible for the formation of the ER body.
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[Journal Article] Arabidopsis KAM2/GRV2 Is required for proper endosome formation and functions in vacuolar sorting and determination of the embryo growth axis.2007
Author(s)
Tamura, K., Takahashi, H., Kunieda, T., Fuji, K., Shimada, T., Hara-Nishimura, I.
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Journal Title
Plant Cell 19
Pages: 320-332
Peer Reviewed
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[Journal Article] AtVAM3 is required for normal specification of idioblasts, myrosin cells.2006
Author(s)
Ueda, H., Nishiyama, C., Shimada, T., Koumoto, Y., Hayashi, Y., Kondo, M., Takahashi, T., Ohtomo, I., Nishimura, M., Hara-Nishimura, I.
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Journal Title
Plant Cell Physiol. 47
Pages: 164-175
Peer Reviewed
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[Journal Article] MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana.2006
Author(s)
Li, L., Shimada, T., Takahashi, H., Ueda, H., Fukao, Y., Kondo, M., Nishimura, M., Hara-Nishimura, I.
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Journal Title
Plant Cell 18
Pages: 3535-3547
Peer Reviewed
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[Remarks] 朝日新聞「細胞小器官に必須遺伝子」2008年11月7日
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[Remarks] 日経産業新聞「防虫力つける遺伝子」2008年10月23日
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[Remarks] 科学新聞「植物の新規細胞小器官"ERボディ"の形成機構」2008年10月3日
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