Role of vacuolar network for ion homeostasis in plant cells under environmental adaptation.

2008 Fiscal Year Final Research Report

• Project/Area Number: 16085204
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kobe University
• Principal Investigator: MIMURA Tetsuro Kobe University, 大学院・理学研究科, 教授 (20174120)
• Co-Investigator(Kenkyū-buntansha): MAESHIMA Masayoshi 名古屋大学, 大学院・生命農学研究科, 教授 (80181577) ; FUKAKI Hidehiro 神戸大学, 大学院・理学研究科, 准教授 (80324979)
• Project Period (FY): 2004 – 2008
• Keywords: 液胞 / 生体膜輸送系 / H+ポンプ / イオンホメオスタシス / 耐塩性 / シロイヌナズナ / 亜鉛イオン / 小胞輸送

Research Abstract

植物細胞の生理機能を支える細胞内低分子環境のうち、無機イオンとしてのH^+、リン酸、Na^+、Ca^<2+>、Znなどに注目し、外部環境の変化に対して、イオン濃度がどのように制御されているかを、特に液胞および液胞小胞系の機能との関連で解析した。その結果、1.高塩環境下における液胞への塩蓄積機構と液胞構造のダイナミックな変化、2.イノシトールリン酸の代謝と液胞への蓄積機構、3.Ca^<2+>ホメオスタシスの分子機構、4.亜鉛集積と液胞ダイナミズム、5.液胞膜H^+輸送ピロホスファターゼの機能の詳細を明らかにすることに成功した。

Research Products

• [Journal Article] Seeing Is Believing: On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics. (2009)
  • Author(s): Mano S., Miwa T., Nishikawa S., Mimura, T., Nishimura M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2000-2014
  • Peer Reviewed
• [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. (2009)
  • Author(s): Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, M. -H., Terao-Morita, M., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H., Mimura, T.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] NIP1;1, an aquaporin homolog, determines the arsenite sensitivity of Arabidopsis thaliana. (2009)
  • Author(s): Kamiya, T., Tanaka, M., Mitani, N., Ma, J.F., Maeshima, M., Fujiwara, T.
  • Journal Title: J. Biol. Chem. 284 Pages: 2114-2120
  • Peer Reviewed
• [Journal Article] AtHMA1 contributes to detoxification of excess Zn(II) in Arabidopsis. (2009)
  • Author(s): Kim, Y. -Y., Choi, H., Segami, S., Cho, H. -T., Martinoia, E., Maeshima, M., Lee, Y.
  • Journal Title: Plant J. 58 Pages: 737-753
• [Journal Article] A mutant strain Arabidopsis that lacks vacuolar membrane zinc transporter MTP1 revealed the latent tolerance to excessive zinc. (2009)
  • Author(s): Kawachi, M., Mori, H., Kobae, Y., Tomioka, R., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1156-1170
  • Peer Reviewed
• [Journal Article] Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis. (2008)
  • Author(s): Mitsuhashi N., Kondo M., Nakaune S., Ohnishi M., Hayashi M., Hara-Nishimura I., Richardson A., Fukaki H., Nishimura M., Mimura, T.
  • Journal Title: J. Exp. Bot. 59 Pages: 3069-3076
  • Peer Reviewed
• [Journal Article] Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana. (2008)
  • Author(s): Uehara T., Okushima Y., Mimura, T., Tasaka M., Fukaki H.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1025-1083
• [Journal Article] Characterization of a tobacco TPK-type K+ channel as a novel tonoplast K+ channel using yeast tonoplast. (2008)
  • Author(s): Hamamoto, S., Marui, J., Matsuoka, K., Higashi, K., Igarashi, K., Nakagawa, T., Mori, Y., Murata, Y., Nakanishi, Y., Maeshima, M., Yabe, I., Uozumi, N.
  • Journal Title: J. Biol. Chem. 283 Pages: 1911-1920
• [Journal Article] Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn2+/H+ antiporter of Arabidopsis thaliana, stimulates the transport activity. (2008)
  • Author(s): Kawachi, M., Kobae, Y., Mimura T., Maeshima, M.
  • Journal Title: J. Biol. Chem. 283 Pages: 8374-8383
  • Peer Reviewed
• [Journal Article] The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2. (2008)
  • Author(s): Lee, M., Choi, Y., Burla, B., Kim, Y. -Y., Jeon, B., Maeshima, M., Yoo, J. -Y., Martinoia, M., Lee, Y.
  • Journal Title: Nature Cell Biol. 10 Pages: 1217-1223
  • Peer Reviewed
• [Journal Article] Protein chemical properties of plasma membrane associated cation-binding protein AtPCaP1 in Arabidopsis thaliana. (2008)
  • Author(s): Nagasaki, N., Miyano, M., Maeshima, M.
  • Journal Title: J. Biochem. 144 Pages: 487-497
  • Peer Reviewed
• [Journal Article] Plant Genetic Engineering vol 9: Plant membrane and vacuolar transporters (2008)
  • Author(s): Mimura T., Ohnishi M., Shimaoka T., Tomizawa K.
  • Journal Title: Proteome analysis of vacuolar membrane 45 Pages: 301-343
  • Peer Reviewed
• [Journal Article] ER membrane aquaporins in plants (2008)
  • Author(s): Maeshima, M., Ishikawa, F.
  • Journal Title: Pflugers Archiv, -Eur. J. Physiol. 456 Pages: 709-716
  • Peer Reviewed
• [Journal Article] Inorganic phosphate uptake in intact vacuoles isolated from suspension cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status (2007)
  • Author(s): hnishi M., Mimura, T., Tsujimura T., Mitsuhashi M., Washitani-Nemoto S., Maeshima M., Martinoia E.
  • Journal Title: Planta 225 Pages: 711-718
  • Peer Reviewed
• [Journal Article] Transcriptional induction of two genes for CCaPs, novel cytosolic proteins, in Arabidopsis thaliana in the dark. (2007)
  • Author(s): Ide, Y., Tomioka, R., Ouchi, Y., Kamiya, T., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 48 Pages: 54-65
  • Peer Reviewed
• [Journal Article] The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance (2007)
  • Author(s): Kim, D., Bovet, L., Maeshima, M., Martinoia, E., Lee, Y.
  • Journal Title: Plant J. 50 Pages: 207-218
  • Peer Reviewed
• [Journal Article] Essential amino acid residues in the central transmembrane domains for energy coupling of the H+-pyrophosphatase of Streptomyces coelicolor A3(2) determined by random and site-directed mutagenesis. (2007)
  • Author(s): Hirono, M., Nakanishi, Y., Maeshima, M.
  • Journal Title: Biochim. Biophys. Acta - Bioenerg. 1767 Pages: 930-939
  • Peer Reviewed
• [Journal Article] Vacuolar transporters and their essential role in plant metabolism. (2007)
  • Author(s): Martinoia, E., Maeshima, M., Neuhaus, E.
  • Journal Title: J. Exp. Bot. 58 Pages: 83-102
  • Peer Reviewed
• [Journal Article] Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension cultured cells. (2006)
  • Author(s): Kobae, Y., Mizutani, M., Segami, S., Maeshima, M.
  • Journal Title: Biosci. Biotechnol. Biochem. 70 Pages: 980-987
  • Peer Reviewed
• [Journal Article] Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection. (2006)
  • Author(s): Kobae, Y., Yoshioka, H., Sekino, T., Nakagawa, T., Martinoia, E., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 47 Pages: 309-318
  • Peer Reviewed
• [Journal Article] Salt stress and glycolytic regulation in suspension-cultured cells of the mangrove tree, Bruguiera sexangula. (2005)
  • Author(s): Suzuki, M., Hashioka, A., Mimura, T., Ashihara H.
  • Journal Title: Physiol. Plant. 123 Pages: 246-253
  • Peer Reviewed
• [Journal Article] Phytic acid synthesis and vacuolar accumulation in suspension-cultured cells of Catharanthus roseus induced by high concentration of Pi and cations. (2005)
  • Author(s): Mitsuhashi, N., Ohnishi, M., Sekiguchi, Y., Kwon, Y. -U., Chang, Y. -T., Chung, S. -K., Inoue, Y., Reid, R.J., Yagisawa, H., Mimura, T.
  • Journal Title: Plant Physiol. 138 Pages: 1607-1614
  • Peer Reviewed
• [Journal Article] Development of a comprehensive analytical method for phosphate metabolites in plants by ion chromatography coupled with tandem mass spectrometry. (2005)
  • Author(s): Sekiguchi, Y., Mitsuhashi, N., Kokaji, T., Miyakoda, H., Mimura, T.
  • Journal Title: J. Chromato. A 1085 Pages: 131-136
  • Peer Reviewed
• [Journal Article] Transcription switches for protoxylem and metaxylemvessel formation. (2005)
  • Author(s): Kubo, M., Udagawa, M., Nishikubo, N., Horiguchi, G., Yamaguchi, M., Ito, J., Mimura, T., Fukuda, H., Demura, T.
  • Journal Title: Genes Dev. 19 Pages: 1855-1860
  • Peer Reviewed
• [Journal Article] Oligomerization of the H^+-pyrophosphatase and its structural and functional consepuences (2005)
  • Author(s): Mimura, H., Nakanishi, Y., Maeshima, M.
  • Journal Title: Biochim. Biopys. Acta-Bioenerg. 1708 Pages: 393-403
  • Peer Reviewed
• [Journal Article] Disulfide bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implication in redox control and structure. (2005)
  • Author(s): Mimura, H., Nakanishi, Y., Maeshima, M.
  • Journal Title: FEBS Lett. 579 Pages: 3625-3631
  • Peer Reviewed
• [Journal Article] Identification of 33 rice aquaporin genes and analysis of their expression and function. (2005)
  • Author(s): Sakurai, J., Ishikawa, F., Yamaguchi, T., Uemura, M., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1568-1577
  • Peer Reviewed
• [Journal Article] Expression profile of rice cation/H+ exchanger family and heterologous expression in yeast. (2005)
  • Author(s): Kamiya, T., Akahori, T., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1735-1740
  • Peer Reviewed
• [Journal Article] Three SIP aquaporins of Arabidopsis are localized in the ER membrane and expressed in a tissue- and cell-specific manner (2005)
  • Author(s): Ishikawa, F., Suga, S., Uemura, T., Sato, M.H., Maeshima, M.
  • Journal Title: FEBS Lett. 579 Pages: 5814-5820
  • Peer Reviewed
• [Journal Article] Analysis of sugar phosphates in plants by ion chromatography with pulsed amperometric detection combined with a titanium oxide column. (2004)
  • Author(s): Sekiguchi, Y., Mitsuhashi, N., Inoue, Y., Yagisawa, H., Mimura, T.
  • Journal Title: J. Chromato. A 1039 Pages: 71-76
  • Peer Reviewed
• [Journal Article] Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. (2004)
  • Author(s): Shimaoka, T., Ohnishi, M., Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K., Mimura, T.
  • Journal Title: Plant Cell Physiol. 45 Pages: 672-683
  • Peer Reviewed
• [Journal Article] Quantification of polyphosphate: different sensitivities to short-chain polyphosphate using enzymatic and colorimetric methods as revealed by ion chromatography. (2004)
  • Author(s): Ohtomo, R., Sekiguchi, Y., Mimura, T., Saito, M., Ezawa, T.
  • Journal Title: Anal. Biochem. 328 Pages: 139-146
• [Journal Article] An assessment of the usefulness of solution culture in screening for phosphorus efficiency in wheat. (2004)
  • Author(s): Hayes, J., Zhu, Y., Mimura, T., Reid, R.
  • Journal Title: Plant and Soil 261 Pages: 91-97
• [Journal Article] Vacuolar sulfate transporters are essential determinants controlling internal distribution of sulfate in Arabidopsis. (2004)
  • Author(s): Kataoka, T., Watanabe-Takahashi, A., Hayashi, N., Ohnishi, M., Mimura, T., Buchner, P., Hawkesford, M.J., Yamaya, T., Takahashi, H.
  • Journal Title: Plant Cell 16 Pages: 2693-2704
  • Peer Reviewed
• [Journal Article] Residues in internal repeats of the rice cation/H+ exchanger are involved in the transport and selection of cations. (2004)
  • Author(s): Kamiya, T., Maeshima, M.
  • Journal Title: J. Biol. Chem. 279 Pages: 812-819
  • Peer Reviewed
• [Journal Article] Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+- pyrophosphatase, H+-ATPase subunit A and Na+/H+ antiporter from barley. (2004)
  • Author(s): Fukuda, A., Chiba, K., Maeda, M., Nakamura, A., Maeshima, M., Tanaka, Y.
  • Journal Title: J. Exp. Bot. 55 Pages: 585-594
  • Peer Reviewed
• [Journal Article] Water channel activity of radish plasma membrane aquaporins heterologously expressed in yeast and their modification by site-directed mutagenesis. (2004)
  • Author(s): Suga, S., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 823-830
  • Peer Reviewed
• [Journal Article] Membrane topology of the H+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. (2004)
  • Author(s): Mimura, H., Nakanishi, Y., Hirono, M., Maeshima, M.
  • Journal Title: J. Biol. Chem. 279 Pages: 35106-35112
  • Peer Reviewed
• [Presentation] 植物液胞のポストゲノム解析 (2009)
  • Author(s): 三村徹郎
  • Organizer: 第4回メタボロームシンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 20091118-20091119
• [Presentation] Structure-function relationship and physiological meanings of H+-pyrophosphatase. (2009)
  • Author(s): Maeshima M.
  • Organizer: The XII Meeting of the SBFV (The Brazilian Society of Plant Physiology)
  • Place of Presentation: Fortaleza, Brazi
  • Year and Date: 20090907-20090912
• [Presentation] Post genome analysis of vacuolar function and control of plant metabolism. (2008)
  • Author(s): Mimura T.
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama
  • Year and Date: 20080715-20080718
• [Presentation] Structure-function relationship of AtMTP1, a vacuolar Zn2+/H+ antiporter and defense mode against zinc stress in atmtp1 mutant plant. The GRL Symposium on Phytoremediation. (2008)
  • Author(s): Maeshima M.
  • Organizer: Pohang University of Science and Technology
  • Place of Presentation: Pohang
  • Year and Date: 20080612-20081614
• [Presentation] Aquaporins localized to ER membrane in Arabidopsis thaliana. (2007)
  • Author(s): Maeshima M.
  • Organizer: The 5th International Conference of Aquaporin.
  • Place of Presentation: Nara, Japan
  • Year and Date: 20070713-20070716
• [Presentation] On a long journey to understand a simple enzyme, vacuolar H+-pyrophosphatase, with a companion. (2007)
  • Author(s): Maeshima M.
  • Organizer: The 1st GRL Conference: Plants for Environment.
  • Place of Presentation: Pohang University of Science and Technology, Korea
  • Year and Date: 20070522-20070523
• [Presentation] Functional structure of H+-pump pyrophosphatase that uses a low cost substrate. (2007)
  • Author(s): Maeshima M.
  • Organizer: International Symposium on Membrane Transport as a Universal Biological Mechanism
  • Place of Presentation: Kyoto
  • Year and Date: 20070113-20070114
• [Presentation] Involvement of Vacuolar Network in Plant Ion homeostasis. (2006)
  • Author(s): Mimura T., Maeshima M.
  • Organizer: Dynamic Organelles in Plants
  • Place of Presentation: Okazaki
  • Year and Date: 20060614-20060617
• [Presentation] 植物病理応答に関わるABCトランスポータPDR8の発見:細胞死との関連. (2006)
  • Author(s): 前島正義
  • Organizer: 第3回重点共同利用研究ワークショップ「植物の自己防御と細胞死」
  • Place of Presentation: 基礎生物学研究所
  • Year and Date: 20060327-20060328
• [Presentation] Accumulation of mineral nutrients and proton circuit in plant vacuoles. (2006)
  • Author(s): Maeshima M.
  • Organizer: Plant Winter Conference 2006. POSTECH, Pohang University of Science and Technology
  • Place of Presentation: Pohang, Korea
  • Year and Date: 20060205-20060206
• [Presentation] Proton circuit and accumulation of mineral nutrients in plant vacuoles (2005)
  • Author(s): Maeshima M.
  • Organizer: The Symposium on Molecular and Cellular Biology of Plant Storage Function: From Gene to Food.
  • Place of Presentation: Nagoya University
  • Year and Date: 20051128-20051129
• [Presentation] (1) Teaching of Plant Physiology in Japan. (2) Water Channel Aquaporin. (3) Dynamics in structure and function of plant vacuolar transport systems. (2005)
  • Author(s): Maeshima M.
  • Organizer: The 2005 Cross-strait Symposium on Teaching of Plant Physiology and Molecular Biology, at Wuhan University
  • Place of Presentation: Wuhan, P R China
  • Year and Date: 20051014-20051019
• [Presentation] Inositol hexakisphosphate synthesis in plant suspension-cultured cells and measurement of inositol phosphate isomers with anion chromatography. (2005)
  • Author(s): Mimura T.
  • Organizer: Inositol Phosphates in the Soil-Plant-Animal System. at Sun Valley
  • Place of Presentation: Idaho, USA
  • Year and Date: 20050821-20050824
• [Presentation] Tertiary structure and functional regulation of H+-pyrophosphatase. (2005)
  • Author(s): Maeshima M.
  • Organizer: The International Symposium on the Biological Membrane Transport 2005
  • Place of Presentation: Awaji, Japan
  • Year and Date: 20050808-20050810
• [Book] 基礎生物学シリーズ7植物生理学 (2009)
  • Author(s): 三村徹郎
  • Publisher: 化学同人
• [Book] 植物の生化学・分子生物学 (2005)
  • Author(s): 前島正義, 杉山達夫;監訳岡田清孝, 内藤哲, 中村研三, 長谷俊治, 福田裕穂, 前島正義
  • Total Pages: 101-142
  • Publisher: 学会出版センター
• [Remarks] 研究代表者三村徹郎
  • URL: http://www.research.kobe-u.ac.jp/fsci-mimura/
• [Remarks] 研究分担者前島正義
  • URL: http://celld.agr.nagoya-u.ac.jp/Cell_Dynamics_HP_J.html
• [Remarks] 研究分担者深城英弘
  • URL: http://www.research.kobe-u.ac.jp/fsci-fukaki/
• [Patent(Industrial Property Rights)] 高等植物の糖リン酸の網羅的分析法 (2008)
  • Inventor(s): 三村徹郎、関口陽子、斉藤和季
  • Industrial Property Rights Holder: 三村徹郎、関口陽子、斉藤和季、独立行政法人科学技術振興機構
  • Patent Publication Number: 特開2008-122404(P2008-122404A)
  • Filing Date: 2008-05-29