Environmental acclimation of chloroplasts

2008 Fiscal Year Final Research Report

• Project/Area Number: 16085206
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2007-2008); Kyushu University (2004-2006)
• Principal Investigator: SHIKANAI Toshiharu Kyoto University, 大学院・理学研究科, 教授 (70273852)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Yoshichika 九州大学, 農学研究院, 教授 (90087594) ; TSUYAMA Michiuto 九州大学, 農学研究院, 助教 (10380552)
• Project Period (FY): 2004 – 2008
• Keywords: 葉緑体 / RNA編集 / 環境適応 / PPRタンパク質 / 光合成 / プラスチド / シロイヌナズナ / 遺伝学

Research Abstract

植物のRNA編集はRNA上で特定のCをUに書き換える反応で、プラスチドとミトコンドリアで頻繁に見られる。本研究では、プラスチドのRNA編集においてPPRタンパク質が標的決定因子として機能することを明らかにした。さらにPPRタンパク質のドメイン構造の解析から、プラスチドでのRNA編集装置の二成分(PPRタンパク質と編集酵素)モデルを提唱した。

Research Products

• [Journal Article] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. (2009)
  • Author(s): Okuda K, Chateigner-Boutin A-L, Nakamura T, Delannoy E, Sugita M, Myouga F, Motohashi R, Shinozaki K, Small I, Shikanai T
  • Journal Title: Plant Cell 21 Pages: 147-56
  • Peer Reviewed
• [Journal Article] SPL7 is a central regulator for copper homeostasis in Arabidopsis. (2009)
  • Author(s): Yamasaki H, Hayashi M, Fukazawa M, Kobayashi Y, Shikanai T
  • Journal Title: Plant Cell 21 Pages: 347-61
  • Peer Reviewed
• [Journal Article] How do plants respond to copper deficiency? (2008)
  • Author(s): Yamasaki H, Pilon M, Shikanai T
  • Journal Title: Plant Signaling & Behavior 3-4 Pages: 231-2
  • Peer Reviewed
• [Journal Article] Characterization of factors affecting the activity of photosystem I cyclic electron transport in chloroplasts. (2008)
  • Author(s): Okegawa Y, Kagawa Y, Kobayashi Y, Shikanai T
  • Journal Title: Plant Cell Physiol 49 Pages: 825-34
  • Peer Reviewed
• [Journal Article] CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2008)
  • Author(s): Shimizu H, Peng L, Myouga F, Motohashi R, Shinozaki K, Shikanai T
  • Journal Title: Plant Cell Physiol 49 Pages: 835-42
  • Peer Reviewed
• [Journal Article] Amino acid sequence variations in Nicotiana CRR4 orthologs determine the species-specific efficiency of RNA editing in plastids. (2008)
  • Author(s): Okuda K, Habata Y, Kobayashi Y, Shikanai T
  • Journal Title: Nucleic Acids Res 36 Pages: 6155-64
  • Peer Reviewed
• [Journal Article] The chloroplast NAD(P)H dehydrogenase complex interacts with photosystem I in Arabidopsis. (2008)
  • Author(s): Peng L, Shimizu H, Shikanai T
  • Journal Title: J Biol Chem 283 Pages: 34873-9
  • Peer Reviewed
• [Journal Article] Cyclic electron transport around photosystem I ; genetic approaches. (2007)
  • Author(s): Shikanai T
  • Journal Title: Annu Rev Plant Biol 58 Pages: 199-217
  • Peer Reviewed
• [Journal Article] Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing. (2007)
  • Author(s): Okuda K, Myouga F, Motohashi R, Shinozaki K, Shikanai T
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 8178-83
  • Peer Reviewed
• [Journal Article] The NAD(P)H dehydrogenase complex in photosynthetic organisms : Subunit composition and physiological function. (2007)
  • Author(s): Shikanai T
  • Journal Title: Func Plant Sci Biotech 1 Pages: 129-37
  • Peer Reviewed
• [Journal Article] Regulation of copper homeostasis by microRNA in Arabidopsis. (2007)
  • Author(s): Yamasaki H, Abdel-Ghany SE, Cohu CM, Kobayashi Y, Shikanai T, Pilon M
  • Journal Title: J Biol Chem 282 Pages: 16369-78
  • Peer Reviewed
• [Journal Article] Dihydrodipicolinate reductase-like protein, CRR1, is essential for chloroplast NAD(P)H dehydrogenase in Arabidopsis. (2007)
  • Author(s): Shimizu H, Shikanai T
  • Journal Title: Plant J. 52 Pages: 539-47
  • Peer Reviewed
• [Journal Article] Balanced PGR5 level is required for chloroplast development and optimum operation of cyclic electron transport around photosystem I. (2007)
  • Author(s): Okegawa Y, Long TA, Iwano M, Takayama S, Kobayashi Y, Covert SF, Shikanai T
  • Journal Title: Plant Cell Physiol 48 Pages: 1462-71
  • Peer Reviewed
• [Journal Article] RNA editing in plant organelles : machinery, physiological function and evolution. (2006)
  • Author(s): Shikanai T
  • Journal Title: Cell Mol Life Sci 63 Pages: 698-708
  • Peer Reviewed
• [Journal Article] CRR6 is a novel factor required for accumulation of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2006)
  • Author(s): Munshi MK, Kobayashi Y, Shikanai T
  • Journal Title: Plant Physiol 141 Pages: 737-44
  • Peer Reviewed
• [Journal Article] A pentatricopeptide repeat protein is a site-recognition factor in chloroplast RNA editing. (2006)
  • Author(s): Okuda K, Nakamura T, Sugita M, Shimizu T, Shikanai T
  • Journal Title: J Biol Chem 281 Pages: 37661-7
  • Peer Reviewed
• [Journal Article] A eukaryotic factor required for accumulation of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2006)
  • Author(s): Muraoka R, Okuda K, Kobayashi Y, Shikanai T
  • Journal Title: Plant Physiol 142 Pages: 1683-9
  • Peer Reviewed
• [Journal Article] A pentatricopetide repeat protein is essential for RNA editing in chloroplasts. (2005)
  • Author(s): Kotera E, Tasaka M, Shikanai T
  • Journal Title: Nature 433 Pages: 326-30
  • Peer Reviewed
• [Journal Article] Physiological requirements of the nonmevalonate pathway for photo-acclimation in Arabidopsis (2005)
  • Author(s): Hojo M, Tasaka M, Shikanai T
  • Journal Title: Plant Biotech 22 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. (2005)
  • Author(s): Abdel-Ghany SE, Muller-Moule P, Niyogi KK, Pilon M, Shikanai T
  • Journal Title: Plant Cell 17 Pages: 1233-51
  • Peer Reviewed
• [Journal Article] The pgr1 mutation in the Rieske subunit of the cytochrome b6f complex does not affect PGR5-dependent cyclic electron transport around photosystem I. (2005)
  • Author(s): Okegawa Y, Tsuyama M, Kobayashi Y, Shikanai T
  • Journal Title: J Biol Chem 280 Pages: 28332-6
  • Peer Reviewed
• [Journal Article] Identification of a novel protein CRR7 required for the stabilization of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2005)
  • Author(s): Munshi MK, Kobayashi Y, Shikanai T
  • Journal Title: Plant J 44 Pages: 1036-44
  • Peer Reviewed
• [Journal Article] Cyclic electron transport through photosystem I. (2005)
  • Author(s): Munekage Y, Shikanai T
  • Journal Title: Plant Biotech 22 Pages: 361-9
  • Peer Reviewed
• [Presentation] 葉緑体におけるPPRタンパク質の多様化した機能 (2009)
  • Author(s): 奥田賢治, Chateigner-Boutin A-L, 中村崇裕, Delannoy E, 杉田護, 明賀史純, 本橋令子, 篠崎一雄, Small I, 鹿内利治
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 20090000
• [Presentation] Mechanism of RNA editing in plastids. (2008)
  • Author(s): Shikanai T
  • Organizer: The Plant & Animal Genome XVI Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 20080000
• [Presentation] Machinery of RNA editing contains PPR proteins in plastids. (2008)
  • Author(s): Shikanai T
  • Organizer: Plant winter Conference
  • Place of Presentation: POSTEC, Korea
  • Year and Date: 20080000
• [Presentation] 葉緑体RNA編集を行う装置 (2008)
  • Author(s): 鹿内利治
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] タバコ祖先種におけるRNA編集システムの比較解析 (2008)
  • Author(s): 奥田賢治, 羽畑優哉, 小林善親, 鹿内利治
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20080000
• [Presentation] Mechanism of RNA editing in plastids. Gordon Research Conference (2008)
  • Author(s): Shikanai T
  • Organizer: Mitochondria & Chloroplasts
  • Place of Presentation: Bidderford, USA
  • Year and Date: 20080000
• [Presentation] Mechanism of RNA editing in plastids. (2008)
  • Author(s): Shikanai T
  • Organizer: Japan-Swiss Workshop
  • Place of Presentation: Nara
  • Year and Date: 20080000
• [Presentation] 葉緑体RNA編集に関わるPPRタンパク質は機能的に保存されたドメイン構造を持つ (2007)
  • Author(s): 奥田賢治, 明賀史純, 本橋令子, 篠崎一雄, 鹿内利治
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 松山
  • Year and Date: 20070000
• [Presentation] 葉緑体RNA編集が異常なシロイヌナズナcrr4のサプレッサー変異株の解析 (2007)
  • Author(s): 北村后希, 奥田賢治, 小林善親, 鹿内利治
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 松山
  • Year and Date: 20070000
• [Presentation] Function of PPR proteins as a trans-factor in chloroplast RNA editing. (2007)
  • Author(s): Okuda K, Shikanai T
  • Organizer: ICPMB 2007
  • Place of Presentation: Nara
  • Year and Date: 20070000
• [Presentation] PPR proteins function as a trans-factor in chloroplast RNA editing. (2007)
  • Author(s): Okuda K, Shikanai T
  • Organizer: International Congress of Photosynthesis
  • Place of Presentation: Glasgow, UK
  • Year and Date: 20070000
• [Presentation] シロイヌナズナPPRタンパク質CRR4による葉緑体RNA編集機構の解明 (2006)
  • Author(s): 奥田賢治, 清水敏之, 鹿内利治
  • Organizer: 第47回日本植物生理学会年会
  • Place of Presentation: 筑波
  • Year and Date: 20060000
• [Presentation] Machinery of RNA editing in the chloroplast. (2006)
  • Author(s): Shikanai T
  • Organizer: The 53^<rd> NIBB Conference
  • Place of Presentation: Okazaki
  • Year and Date: 20060000
• [Presentation] Machinery of RNA editing includes a PPR protein in plastids. (2006)
  • Author(s): Shikanai T
  • Organizer: Japanese-Finnish Seminar
  • Place of Presentation: Nara
  • Year and Date: 20060000
• [Presentation] シロイヌナズナ核遺伝子CRR4は葉緑体遺伝子ndhDのRNA編集に関わる (2005)
  • Author(s): 小寺栄見, 田坂昌生, 鹿内利治
  • Organizer: 第46回植物生理学会年会
  • Place of Presentation: 新潟
  • Year and Date: 20050000
• [Presentation] Function of PPR proteins in the maturation of chloroplast RNA. (2005)
  • Author(s): Shikanai T
  • Organizer: ICPMB 2005
  • Place of Presentation: Obernai, France
  • Year and Date: 20050000
• [Book] Machinery of RNA editing in plant organelles. "RNA and DNA editing : Molecular mechanisms and their integration into biological system. (2008)
  • Author(s): Shikanai T, Obokata J
  • Total Pages: 99-119
• [Book] 植物の光環境適応戦略 生物と化学 44 (2006)
  • Author(s): 鹿内利治
  • Total Pages: 121-7
• [Book] なぜ植物はRNA編集をやめないのか 蛋白質核酸酵素 51 (2006)
  • Author(s): 鹿内利治
  • Total Pages: 229-36
• [Book] 高等植物の葉緑体におけるRNA editingのメカニズム 実験医学 23 (2005)
  • Author(s): 鹿内利治, 小寺栄見, 田坂昌生
  • Total Pages: 1213-5
• [Book] RNA編集 ゲノム進化の痕跡? 蛋白質核酸酵素 50 (2005)
  • Author(s): 鹿内利治
  • Total Pages: 1858-9