Mechanisms of sex determination and gonadal sex differentiation in fish

2008 Fiscal Year Final Research Report

• Project/Area Number: 16086213
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: NAGAHAMA Yoshitaka National Institute for Basic Biology, 生殖生物学研究部門, 特任教授 (50113428)
• Project Period (FY): 2004 – 2008
• Keywords: 性決定 / 性分化 / 魚類 / 性決定遺伝子 / エストロゲン / DMRT1 / Foxl2 / 芳香化酵素 / メダカ

Research Abstract

本研究は、2種の雌雄異体魚(メダカとティラピア)を実験材料として、脊椎動物における性決定と生殖腺の性分化の分子メカニズムを解明することを目的として行った。その結果、DMYはメダカの雄への分化に必要かつ十分であること、魚類では、DMRT1は精巣の形成に不可欠であること、さらにFoxl2はcyp19a1(卵巣型芳香化酵素)遺伝子の発現促進を介して卵巣形成に重要な役割を果たすことが明らかとなった。

Research Products

• [Journal Article] Stability in aromatase immunoreactivity of steroid-producing cells during early development of XX gonads of the Nile tilapia, Oreochromis niloticus : An organ culture study (2008)
  • Author(s): Sakai, F., Kobayashi, T., Matsuda, M. and Nagahama, Y
  • Journal Title: Zool. Sci 25 Pages: 344-348
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of DMRT1 and Sox9 during gonadal differentiation and hormone-induced sex reversal in the teleost fish Nile tilapia (Oreochromis niloticus) (2008)
  • Author(s): Kobayashi, T., Kajiura-Kobayashi, H., Guan, G. and Nagahama, Y
  • Journal Title: Dev. Dyn 237 Pages: 297-306
  • Peer Reviewed
• [Journal Article] Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus (2008)
  • Author(s): Ijiri, S., Kaneko, H., Kobayashi, T., Wang, D.S., Sakai, F., Paul-Prasanth, B., Nakamura M. and Nagahama, Y
  • Journal Title: Biol. Reprod 78 Pages: 333-341
  • Peer Reviewed
• [Journal Article] Characterization, expression and transcriptional regulation of P450c17-I and -II in the medaka, Oryzias latipes. Biochem (2007)
  • Author(s): Zhou, L.Y., Wang, D.S., Shibata, Y., Paul-Prasanth, B., Suzuki, A. and Nagahama, Y
  • Journal Title: Biophys. Res. Comm 362 Pages: 619-624
  • Peer Reviewed
• [Journal Article] A novel type of P450c17 lacking the lyase activity is responsible for C21-steroid biosynthesis in the fish ovary and head kidney (2007)
  • Author(s): Zhou, L.Y., Wang, D.S., Kobayashi, T., Yano, A., Paul-Prasanth, B., Suzuki, A., Sakai, F. and Nagahama, Y
  • Journal Title: Endocrinology 148 Pages: 4288-4291
  • Peer Reviewed
• [Journal Article] DMY gene induces male development in genetically female (XX) medaka fish. Proc. (2007)
  • Author(s): Matsuda, M., Shinomiya, S., Kinoshita, M., Suzuki, A., Kobayashi, T., Paul-Prasanth, B., Lau, E.L., Hamaguchi, S., Sakaizumi, M. and Nagahama, Y
  • Journal Title: Natl. Acad. Sci. USA 104 Pages: 3865-3870
  • Peer Reviewed
• [Journal Article] Foxl2 up-regulates aromatase gene transcription female-specifically by binding to the promoter as well as interacting with Ad4BP/SF-1 (2007)
  • Author(s): Wang, D.S., Kobayashi, T., Zhou, L.Y., Paul-Prasanth, B., Ijiri, S., Sakai, F., Okubo, K., Morohashi, K. and Nagahama, Y
  • Journal Title: Mol. Endocrinol 21 Pages: 712-725
  • Peer Reviewed
• [Journal Article] Knock-down of DMY initiates female pathway in the genetic male medaka, Oryzias latipes (2006)
  • Author(s): Paul-Prasanth, B., Matsuda, M., Lau, E.L., Suzuki, A., Sakai, F., Kobayashi, T. and Nagahama, Y
  • Journal Title: Biochem. Biophys. Res. Comm 351 Pages: 815-819
  • Peer Reviewed
• [Journal Article] Changes in steroidogenic enzyme and steroidogenic acute regulatory protein messenger RNAs in ovarian follicles during ovarian development of rainbow trout (Oncorhynchus mykiss) (2005)
  • Author(s): Nakamura, I., Evans, J.C., Kusakabe, M., Nagahama, Y. and Young, G.
  • Journal Title: Gen. Comp. Endocrinol 144 Pages: 224-231
  • Peer Reviewed
• [Journal Article] Cloning, expression and characterization of three types of 17β-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus (2005)
  • Author(s): Zhou, L.Y., Wang, D.S., Senthilkumaran, S., Yoshikuni, M., Shibata, Y., Kobayashi, T., Sudhakumari, C.C. and Nagahama, Y
  • Journal Title: J. Mol. Endocrinol 35 Pages: 103-116
  • Peer Reviewed
• [Presentation] Sex determination/differentiation and sexual plasticity in fish (2008)
  • Author(s): Nagahama, Y.
  • Organizer: XX Inter- national Congress of Zoology
  • Place of Presentation: Paris, France.
  • Year and Date: 20080826-29
• [Presentation] Sex determination, differentiation and sexual plasticity in fish: Current status and future directions. (2008)
  • Author(s): Nagahama, Y.
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish: Current Status and Future Directions
  • Place of Presentation: University of Hawaii, Honolulu, Hawaii, USA
  • Year and Date: 20080530-0601
• [Presentation] Molecular mechanisms of sex determination/differentiation in fish (2007)
  • Author(s): Nagahama, Y.
  • Organizer: The International Symposium on Oryzias Fish
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 20071212-14
• [Presentation] Hormonal regulation of oocyte maturation and ovulation in fish and starfish-comparative to general (2007)
  • Author(s): Nagahama, Y
  • Organizer: 6th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
  • Place of Presentation: Sliguri, Darjeeling, West Bengal, India
  • Year and Date: 20071210-14
• [Presentation] The second sex-determining gene in vertebrates (2005)
  • Author(s): Nagahama, Y. DMY
  • Organizer: 15th International Congress of Comparative Endocrinology.
  • Place of Presentation: Boston, USA
  • Year and Date: 20050522-27
• [Book] 日本動物学会監修 (2007)
  • Author(s): 長濱嘉孝、井口泰泉共編
  • Total Pages: 256
  • Publisher: 内分泌と生命現象、シリーズ21世紀の動物科学
• [Book] 蛋白質核酸酵素(Vol. 49, 97-134) (2004)
  • Author(s): 長濱嘉孝
  • Publisher: 動物の性はそのように決まるか?