2009 Fiscal Year Final Research Report
Three-dimensional structure analysis of bacterial pheromone receptor and transporter membrane protein and its application to the design of novel bacteriostatic agents
| Project/Area Number |
16087203
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGATA Koji The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (30280788)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Jiro 九州大学, 大学院・農学研究院, 准教授 (40217930)
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| Project Period (FY) |
2004 – 2009
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| Keywords | quorum sensing / Enterococcus faecalis / histidine kinase / peptide pheromone / membrane protein / signal transduction / two component system |
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| Research Abstract |
The gelatinase production by Enterococcus faecalis is regulated by the cyclic peptide pheromone, GBAP. We determined the tertiary structure of GBAP and identified the important residues for its pheromone activity. We also developed GBAP antagonists by Ala mutations based on the structural information. We expressed in Escherichia coli, purified and crystallized recombinant FsrC, the GBAP receptor membrane protein. A crystal of FsrC-GBAP complex diffracted X-rays to 5.5-Aresolution.
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[Journal Article] Biosynthesis of the thiolactone derivative of Enterococcus faecalis gelatinase- biosynthesis activating pheromone by using the Mycobacterium xenopi GyrA mini-intein2010
Author(s)
Yamanaka, Y., Hayakawa, K., Katayama, H., Nishiguchi, K., Sonomoto, K., Nakayama, J., Nagata, K., Tanokura, M
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Journal Title
Peptide Science 2009 (Okamoto K ed. )
Pages: 171-172
Peer Reviewed
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[Journal Article] Siamycin attenuates fsr quorum sensing mediated by gelatinase biosynthesis-activating pheromone in Enterococcus faecalis2007
Author(s)
Nakayama, J., Tanaka, E., Kariyama, R., Nagata, K., Nishiguchi, K., Mitsuhata, R., Uemura, Y., Tanokura, M., Kumon, H., Sonomoto, K
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Journal Title
J. Bacteriol 189
Pages: 1358-1365
Peer Reviewed
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[Presentation] Biosynthesis of the thiolactone derivative of Enterococcus faecalis gelatinase- biosynthesis activating pheromone by using the Mycobacterium xenopi GyrA mini-intein2009
Author(s)
Yamanaka, Y., Hayakawa, K., Katayama, H., Nishiguchi, K., Sonomoto, K., Nakayama, J., Nagata, K., Tanokura, M
Organizer
第46回ペプチド討論会
Place of Presentation
北九州市(福岡県)
Year and Date
20091100
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