2009 Fiscal Year Final Research Report
High resolution X-ray crystal structural analysis of biological macromolecular assemblies
| Project/Area Number |
16087206
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
TSUKIHARA Tomitake Osaka University, 蛋白質研究所, 特任研究員 (00032277)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Eiki 大阪大学, 蛋白質研究所, 助教 (00294132)
TANAKA Hideaki 大阪大学, 蛋白質研究所, 助教 (40346169)
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| Project Period (FY) |
2004 – 2009
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| Keywords | 生体超分子 / 構造 / 機能 / X線結晶構造解析 |
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| Research Abstract |
The goal of this research was to establish strategies by which we overcame limitation of molecular size and resolution in crystal structure analysis of biological macromolecular assemblies. We had two research plans. The first was to locate hydrogen atoms of cytochrome c oxidase with a molecular weight of 420 KDa at 1.6 A resolution. The second was to determine the structure of vault with a molecular weight of 10 MDa.Improving diffraction experiment method at BL44XU of SPring-8, we have successfully collected diffraction data of cytochrome c oxidase at 1.6 A resolution. We confirmed that the data set contained information of hydrogen atom positions. Structures of connexin26 gap junction channel and Exportin-5:RanGTP:pre-miRNA complex were determined by using intensity data sets acquired at the BL44XU.The structure of vault was determined by applying a newly developed method.
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[Journal Article] The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses.2007
Author(s)
Fusamichi Akita, Khoon Tee Chong, Hideaki Tanaka, Eiki Yamasita, Naoyuki Miyazaki, Yuichiro Nakaishi, Mamoru Suzuki, Kazuhiro Namba, Yasuko Ono, Tomitake Tsukihara,, Atsushi Nakagawa
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Journal Title
J. Mol. Biol. 368
Pages: 1469-1483
Peer Reviewed
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[Journal Article] Structures and physiological roles of all the integral lipids of bovine heart cytochrome c oxidase.2007
Author(s)
Kyoko Shinzawa-Itoh, Hiroshi Aoyama, Kazumasa Muramoto, Hirohito Terada, Tsuyoshi Kurauchi, Yoshiki Tadehara, Akiko Yamasaki, Takashi Sugimura, Sadamu Kurono, Kazuo Tsujimoto, Tsunehiro Mizushima, Eiki Yamashita, Tomitake Tsukihara, Shinya Yoshikawa
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Journal Title
EMBO J. 26
Pages: 1713-1725
Peer Reviewed
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[Journal Article] Transcription and the conserved interactions of two major structural proteins of a pair of Phytoreoviruses confirm the mechanism of assembly of the outer capsid layer.2005
Author(s)
Miyazaki, N., Hagiwara, K., Naitow, H., Higashi, T., Cheng, R.H., Tsukihara, T., Nakagawa, A., Omura, T.
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Journal Title
J. Mol. Biol. 345
Pages: 229-237
Peer Reviewed
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[Presentation] The structure of rat liver vault2009
Author(s)
Tomitake Tsukihara
Organizer
AsCA '09 Joint conference of the Asian crystallographic society and Chinese crystallography society
Place of Presentation
Jingyi Hotel, Beijing, China
Year and Date
20091022-20091025
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