Hybrid approach by electron and X-ray structural analysis for large macromolecular assemblies towards pseudo-atomic resolution

2009 Fiscal Year Final Research Report

• Project/Area Number: 16087207
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: NAMBA Keiichi Osaka University, 大学院・生命機能研究科, 教授 (30346142)
• Co-Investigator(Kenkyū-buntansha): IMADA Katsumi 大阪大学, 大学院・生命機能研究科, 准教授 (40346143)
• Project Period (FY): 2004 – 2009
• Keywords: 1分子計測(SMD) / 電子顕微鏡 / ナノバイオ / ナノマシン / 分子モーター

Research Abstract

To elucidate the mechanisms of large biological macromolecular complexes underlying various biological functions and to make use of them in the application for medicine and nanotechnology, it is essential to visualize their 3D structures in atomic detail. Electron cryomicroscopy (cryoEM) and image analysis of large complexes, although difficult to achieve atomic resolution by itself, can be combined with X-ray crystallography of component molecules to build pseudo-atomic models of the complexes. We have improved the resolution and throughput of cryoEM image analysis to increase the accuracy and efficiency of atomic model building of the complexes markedly to facilitate our understanding of the biological mechanisms.

Research Products

• [Journal Article] Conformational change of flagellin for polymorphic supercoiling of the flagellar filament. (2010)
  • Author(s): Maki-Yonekura, S., Yonekura, K., Namba, K.
  • Journal Title: Nature Struct. & Mo. Biol. (in press)
  • Peer Reviewed
• [Journal Article] Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy. Structure (2010)
  • Author(s): Kato, T., Yoshida, D., Miyata, T., Maki, Y., Wada, A., Namba, K.
  • Journal Title: (in press)
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals. (2010)
  • Author(s): Yamaguchi, T., Fujii, T., Abe, Y., Hirai, T., Kang, D., Namba, K., Hamasaki, N., Mitsuoka, K.
  • Journal Title: J. Struct. Biol. 169 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Structure of the cytoplasmic domain of FlhA and implication for flagellar type III protein export. (2010)
  • Author(s): Saijo-Hamano, Y., Imada, K., Minamino, T., Kihara, M., Shimada, M., Kitao, A., Namba, K.
  • Journal Title: Mol. Microbiol. 76 Pages: 260-268
  • Peer Reviewed
• [Journal Article] Proton-conductivity assay of plugged and unplugged MotA/B proton channel by cytoplasmic pHluorin expressed in Salmonella. (2010)
  • Author(s): Morimoto, Y.V., Che, Y., Minamino, T., Namba, K.
  • Journal Title: FEBS Letters 584 Pages: 1268-1272
  • Peer Reviewed
• [Journal Article] Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export. (2010)
  • Author(s): Minamino, T., Shimada, M., Okabe, M., Saijo-Hamano, Y., Imada, K., Kihara, M., Namba, K.
  • Journal Title: J. Bacteriol. 192 Pages: 1929-1936
  • Peer Reviewed
• [Journal Article] Roles of the extreme N-terminal region of FliH for efficient localization of the FliH-FliI complex to the bacterial flagellar type III export apparatus. (2009)
  • Author(s): Minamino, T., Yoshimura, S.D.J., Morimoto, Y.V., Gonzalez-Pedrajo, B., Kami-ike, N., Namba, K.
  • Journal Title: Mol. Microbiol. 74 Pages: 1471-1483
  • Peer Reviewed
• [Journal Article] Specific arrangement of α-helical coiled coils in the core domain of the bacterial flagellar hook for the universal joint function. (2009)
  • Author(s): Fujii, T., Kato, T., Namba, K.
  • Journal Title: Structure 17 Pages: 1485-1493
  • Peer Reviewed
• [Journal Article] Role of a Conserved Prolyl Residue (Pro-173) of MotA in the Mechanochemical Reaction Cycle of the Proton-Driven Flagellar Motor of Salmonella. (2009)
  • Author(s): Nakamura, S., Morimoto, Y.V., Kami-ike, N., Minamino, T., Namba, K.
  • Journal Title: J. Mol. Biol 393 Pages: 300-307
  • Peer Reviewed
• [Journal Article] ATP-induced FliI hexamerization facilitates bacterial flagellar protein export. (2009)
  • Author(s): Kazetani, K., Minamino, T., Miyata, T., Kato, T., Namba, K.
  • Journal Title: Biochemical and Biophysical Research Communications 388 Pages: 323-327
  • Peer Reviewed
• [Journal Article] Stator assembly and activation mechanism of the flagellar motor by the Periplasmic region of MotB. (2009)
  • Author(s): Kojima, S., Imada, K., Sakuma, M., Sudo, Y., Kojima, C., Minamino, T., Homma, M., Namba, K.
  • Journal Title: Mol. Microbiol. Vol.73 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray analysis of FliT, a bacterial flagellar substrate-specific export chaperone. (2009)
  • Author(s): Kinoshita, M., Yamane, M., Matsunami, H., Minamino, T., Namba, K., Imada, K.
  • Journal Title: Acta Cryst. F65 Pages: 825-828
  • Peer Reviewed
• [Journal Article] High-resolution structural analysis of a DNA nanostructure by cryoEM. (2009)
  • Author(s): Kato, T., Goodman, R.P., Erben, C.M., Turberfield, A.J., Namba, K.
  • Journal Title: NANO Letters Vol.9,No.7 Pages: 2747-2750
  • Peer Reviewed
• [Journal Article] Role of the N-terminal domain of FliI ATPase in bacterial flagellar protein export. (2009)
  • Author(s): Okabe, M., Minamino, T., Imada, K., Namba, K., Kihara, M.
  • Journal Title: FEBS Letters 583 Pages: 743-748
  • Peer Reviewed
• [Journal Article] Effect of Intracellular pH on the Torque-Speed Relationship of Bacterial Proton-Driven Flagellar Motor. (2009)
  • Author(s): Nakamura, S., Kami-ike, N., Yokota, J.P., Kudo, S., Minamino, T.,, Namba, K.
  • Journal Title: J. Mol. Biol. 386 Pages: 332-338
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of FliJ, a cytoplasmic component of the flagellar type III protein export apparatus from Salmonella. (2009)
  • Author(s): Ibuki, T., Shimada, M., Minamino, T., Namba, K., Imada, K.
  • Journal Title: Acta Crystallogr. F65 Pages: 47-50
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of a C-terminal fragment of FlgJ, a putative flagellar rod cap protein of Salmonella. (2009)
  • Author(s): Kikuchi, Y., Matsunami, H., Yamane, M., Imada, K., Namba, K.
  • Journal Title: Acta Crystallogr. F65 Pages: 17-20
  • Peer Reviewed
• [Journal Article] Molecular motors of the bacterial flagella. (2008)
  • Author(s): Minamino, T., Imada, K., Namba, K.
  • Journal Title: Current Opinion in Structural Biology 18 Pages: 693-701
  • Peer Reviewed
• [Journal Article] Mechanisms of type III protein export for bacterial flagellar assembly. (2008)
  • Author(s): Minamino, T., Imada, K., Namba, K.
  • Journal Title: Mol. BioSyst. 4 Pages: 1105-1115
  • Peer Reviewed
• [Journal Article] Pleomorphic configuration of the trimeric capsid proteins of rice dwarf virus that allows formation of both the outer capsid and tubular crystals. (2008)
  • Author(s): Iwasaki, K., Miyazaki, N., Hammar L., Zhu, Y., Omura, T., Wu, B., Sjoborg, F., Yonekura, K., Murata, K., Namba, K., Casper, D.L., Fujiyoshi, Y., Cheng, R.H.
  • Journal Title: J. Mol. Biol. 383 Pages: 252-265
  • Peer Reviewed
• [Journal Article] Suppressor analysis of the MotB(D33E) mutation to probe the bacterial flagellar motor dynamics coupled with proton translocation. (2008)
  • Author(s): Che, Y-S., Nakamura, S., Kojima, S., Kami-ike, N., Namba, K., Minamino, T.
  • Journal Title: J. Bacteriol. 190 Pages: 6660-6667
  • Peer Reviewed
• [Journal Article] Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY. (2008)
  • Author(s): Kojima, S., Shinohara, A., Terashima, H., Yakushi, T., Sakuma, M., Homma, M., Namba, K., Imada, K.
  • Journal Title: PNAS 105 Pages: 7696-7701
  • Peer Reviewed
• [Journal Article] Characterization of the Periplasmic Domain of MotB and Implications for Its Role in the Stator Assembly of the Bacterial Flagellar Motor. (2008)
  • Author(s): Kojima, S., Furukawa, Y., Matsunami, H., Minamino, T., Namba, K.
  • Journal Title: J. Bacteriol. 190 Pages: 3314-3322
  • Peer Reviewed
• [Journal Article] Distinct roles of the ATPase and proton motive force in bacterial flagellar protein export. (2008)
  • Author(s): Minamino, T., Namba, K.
  • Journal Title: Nature 451 Pages: 485-488
  • Peer Reviewed
• [Journal Article] Structure and quantum chemical analysis of NAD+-dependent isocitrate dehydrogenase: Hydride transfer and co-factor specificity. (2008)
  • Author(s): Imada, K., Tamura, T., Takenaka, R., Kobayashi I., Namba, K., Inagaki, K.
  • Journal Title: Proteins: Structure, Function, and Bioinformatics 70 Pages: 63-71
  • Peer Reviewed
• [Journal Article] PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system development. (2007)
  • Author(s): Inoue, M., Kamachi, Y., Matsunami, H., Imada, K., Uchikawa, M., Kondoh H.
  • Journal Title: Genes to Cells 12 Pages: 1049-1061
  • Peer Reviewed
• [Journal Article] Modeling of the F-actin structure. (2007)
  • Author(s): Oda, T., Stegmann, H., Schroder, R.R. Namba, K., Maeda, Y.
  • Journal Title: Adv. Exp. Med. Biol. 592 Pages: 385-401
  • Peer Reviewed
• [Journal Article] Gap compression/extension mechanism of bacterial flagellar hook as the molecular universal joint. (2007)
  • Author(s): Furuta, T., Samatey, F.A., Matsunami, H., Imada, K., Namba, K., Kitao, A.
  • Journal Title: J. Struct. Biol. 157 Pages: 481-490
  • Peer Reviewed
• [Journal Article] Structural similarity between the flagellar type III ATPase FliI and F1-ATPase subunits. Proc. (2007)
  • Author(s): Imada, K., Minamino, T., Tahara, A., Namba, K.
  • Journal Title: Nat'l. Acad. Sci. U.S.A. 104 Pages: 485-490
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio Alginolyticus polar flagellar. (2007)
  • Author(s): Shinohara, A., Sakuma, M., Yakushi, T., Kojima, S., Namba, K., Homma, M., Imada, K.
  • Journal Title: Acta Crystallogr. F63 Pages: 89-92
  • Peer Reviewed
• [Journal Article] Electron cryomicroscopic visualization of PomA/B stator units of the sodium-driven flagellar motor in liposomes. (2006)
  • Author(s): Yonekura, K., Yakushi, T., Atsumi, T., Maki-Yonekura, S., Homma, M., Namba, K.
  • Journal Title: J. Mol. Biol. 357 Pages: 73-81
  • Peer Reviewed
• [Journal Article] The type III flagellar export specificity switch is dependent on FliK ruler and a molecular clock. (2006)
  • Author(s): Moriya, N., Minamino, T., Hughes, K.T., Macnab, R.M., Namba, K.
  • Journal Title: J. Mol. Biol. 359 Pages: 466-477
  • Peer Reviewed
• [Journal Article] a Salmonella flagellar switch protein interacts with FliH, a type III export apparatus component. (2006)
  • Author(s): Gonzalez, P.B., Minamino, T., Kihara, M., Namba, K., Macnab, R.M. FliN
  • Journal Title: Mol. Microbiol. 60 Pages: 984-998
  • Peer Reviewed
• [Journal Article] Switch interactions control energy frustration and multiple flagellar filament structures. (2006)
  • Author(s): Kitao, A., Yonekura, K., Maki-Yonekura, S., Samatey, F.A., Imada, K., Namba, K., Go, N.
  • Journal Title: Proc. Nat'l. Acad. Sci. U.S.A. 103 Pages: 4894-4899
  • Peer Reviewed
• [Journal Article] Oligomerization of the bacterial flagellar ATPase FliI is controlled by its extreme N-terminal Region. (2006)
  • Author(s): Minamino, T., Kazetani, K., Tahara, A., Suzuki, H., Furukawa, Y., Kihara, M., Namba, K.
  • Journal Title: J. Mol. Biol. 360 Pages: 510-519
  • Peer Reviewed
• [Journal Article] Two parts of the T3S4 domain of the hook-length control protein FliK are essential for the substrate specificity switching of the flagellar type III export apparatus. (2006)
  • Author(s): Minamino, T., Ferris H.U., Moriya, N., Kihara, M., Namba, K.
  • Journal Title: J. Mol. Biol. 362 Pages: 1148-1158
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of Salmonella FliI, the ATPase component of the type III flagellar protein-export apparatus. (2006)
  • Author(s): Minamino, T., Imada, K., Tahara, A., Kihara, M., Macnab, R.M., Namba, K.
  • Journal Title: Acta Crystallogr. F62 Pages: 973-975
  • Peer Reviewed
• [Journal Article] partial atomic structure for the flagellar hook of Salmonella typhimurium. (2005)
  • Author(s): Shaikh, T.R., Thomas, D.R., Chen, J.Z., Samatey, F.A., Matsunami, H., Imada, K., Namba, K., DeRosier, D.J. A
  • Journal Title: Proc. Nat'l. Acad. Sci. U.S.A. 102 Pages: 1023-1028
  • Peer Reviewed
• [Journal Article] Position and orientation of phalloidin F-actin determined by X-ray fiber diffraction analysis. (2005)
  • Author(s): Oda, T., Namba, K., Maeda, Y.
  • Journal Title: Biophys. J. 88 Pages: 2727-2736
  • Peer Reviewed
• [Journal Article] Building the atomic model for the bacterial flagellar filament by electron cryomicroscopy and image analysis. (2005)
  • Author(s): Yonekura, K., Maki-Yonekura, S., Namba, K.
  • Journal Title: Structure 13 Pages: 407-412
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the c-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus. (2005)
  • Author(s): Saijo-Hamano, Y., Imada, K., Minamino, T., Kihara, M., Macnab, R.M., Namba, K.
  • Journal Title: Acta Crystallogr. F61 Pages: 599-602
  • Peer Reviewed
• [Journal Article] FlhB regulates ordered export of flagellar components via autocleavage mechanism. (2005)
  • Author(s): Ferris, H.U., Furukawa, Y., Minamino, T., Kroetz, M.B., Kihara, M., Namba, K., Macnab, R.M.
  • Journal Title: J. Biol. Chem. 280 Pages: 41236-41242
  • Peer Reviewed
• [Journal Article] Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex. (2005)
  • Author(s): Iwase, R., Imada, K., Hayashi, F., Uzumaki, T., Morishita, M., Onai, K., Furukawa, Y., Namba, K., Ishiura, M.
  • Journal Title: J. Biol. Chem. 280 Pages: 43141-43149
  • Peer Reviewed
• [Journal Article] Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism. (2004)
  • Author(s): Samatey, F.A., Matsunami, H., Imada, K., Nagashima, S., Shaikh, T.R., Thomas, D.R., Chen, J.Z., DeRosier, D.J., Kitao, A., Namba K.
  • Journal Title: Nature 431 Pages: 1062-1068
  • Peer Reviewed
• [Journal Article] Structural and functional analysis of the c-terminal cytoplasmic domain of FlhA, an integral membrane component of the type III flagellar protein export apparatus in Salmonella. (2004)
  • Author(s): Saijo-Hamano, Y., Minamino, T., Macnab, R.M., Namba, K.
  • Journal Title: J. Mol. Biol. 343 Pages: 457-466
  • Peer Reviewed
• [Journal Article] Domain organization and function of Salmonella FliK, a flagellar hook-length control protein. (2004)
  • Author(s): Minamino, T., Saijo-Hamano, Y., Furukawa, Y., Gonzalez-Pedrajo, B., Macnab, R.M., Namba, K.
  • Journal Title: J. Mol. Biol. 341 Pages: 491-502
  • Peer Reviewed
• [Journal Article] In vitro characterization of FlgB, FlgC, FlgF, FlgG, and FliE, flagellar basal body proteins of Salmonella. (2004)
  • Author(s): Saijo-Hamano, Y., Uchida, N., Namba, K., Oosawa, K.
  • Journal Title: J. Mol. Biol. 339 Pages: 423-435
  • Peer Reviewed
• [Journal Article] Self-assembly and type III protein export of the bacterial flagellum. (2004)
  • Author(s): Minamino, T., Namba, K.
  • Journal Title: J. Mol. Microbiol. and Biotechnol. 7 Pages: 5-17
  • Peer Reviewed
• [Presentation] Molecular mechanisms of self-assembly, switching and motion of the bacterial flagellum. (2010)
  • Author(s): Namba, K.
  • Organizer: Gordon Research Conference on Biomolecular Interactions & Methods
  • Place of Presentation: Hotel Galvez, Texas, U.S.A.
  • Year and Date: 2010-01-19
• [Presentation] Structure of the bacterial flagellum and the mechanism of its self-assembly. (2009)
  • Author(s): Imada, K.
  • Organizer: 4th Conference on Artificial Muscles -5th World Congress on Biomimetics, Artificial Muscles and Nano-Bio-
  • Place of Presentation: Senri Life Science Center, Osaka, Japan
  • Year and Date: 2009-11-26
• [Presentation] Structure of the bacterial flagellum and the mechanism of its self-assembly. (2009)
  • Author(s): Imada, K.
  • Organizer: Joint Conference of the Asian Crystallographic Association and Chinese Crystallography Society
  • Place of Presentation: Jingyi Hotel, Beijing, China
  • Year and Date: 2009-10-23
• [Presentation] Molecular mechanisms of self-assembly, switching and motion of the bacterial flagellum. (2009)
  • Author(s): Namba, K.
  • Organizer: Advanced Electron Microscopy in Nanomedicine Symposium
  • Place of Presentation: UCLA, USA
  • Year and Date: 2009-10-03
• [Presentation] Molecular mechanisms of self-assembly, switching and motion of the bacterial flagellum. (2009)
  • Author(s): Namba, K.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2009-07-31
• [Presentation] Molecular mechanisms of self-assembly and motion of the bacterial flagellum. (2009)
  • Author(s): Namba, K.
  • Organizer: VIII European Symposium of The Protein Society
  • Place of Presentation: Kongresshaus, Zurich, Switzerland
  • Year and Date: 2009-06-15
• [Presentation] Structures and functions of large molecular assemblies. (2009)
  • Author(s): Namba, K.
  • Organizer: Awards Symposium of the 2009 Biophysical Society, Boston Convention and Exhibition Center
  • Place of Presentation: Boston, U.S.A.
  • Year and Date: 2009-03-03
• [Presentation] Molecular mechanisms of self-assembly and motion of the bacterial flagellum. (2009)
  • Author(s): Namba, K.
  • Organizer: Mexico-Japan Workshop "Bacterial Physiology and Biophysics"
  • Place of Presentation: UNAM, Mexico City, Mexico
  • Year and Date: 2009-02-26
• [Presentation] Protein export and ordered self-assembly for the formation of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: Gordon Research Conference on Sensory Transduction in Microorganisms
  • Place of Presentation: Ventura Beach Marriott, Ventura, CA
  • Year and Date: 20080113-20080118
• [Presentation] Molecular mechanisms of self-assembly and its regulation of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: 2008 Keystone Symposium, Frontiers of Structural Biology, Sheraton Steamboat Resort & Steamboat Springs
  • Place of Presentation: Colorado
  • Year and Date: 20080106-20080111
• [Presentation] Molecular mechanisms of self-assembly and protein export of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: 39th NIPS International Symposium & 7th OIB Symposium"Frontiers of Biological Imaging: Synergy of the Advanced Techniques"
  • Place of Presentation: Okazaki Conference Center, Japan
  • Year and Date: 2008-11-11
• [Presentation] DNA Nanostructures visualized by Cryo-EM. (2008)
  • Author(s): Kato, T. Goodman, R.P., Erben, C.M., Turberfield, A.J., Namba, K.
  • Organizer: 9th Asia-Pacific Microscopy Conference (APMC9), International Convention Center Jeju
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2008-11-06
• [Presentation] Molecular mechanisms of self-assembly and its regulation of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: 9th Asia-Pacific Microscopy Conference (APMC9), International Convention Center Jeju
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2008-11-04
• [Presentation] Molecular Mechanisms of Self-Assembly and Its Regulation of the Bacterial Flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: 2nd International Symposium on Bio-nanosystems 2008
  • Place of Presentation: Koshiba Hall, University of Tokyo, Japan
  • Year and Date: 2008-11-02
• [Presentation] Molecular mechanisms of self-assembly and its regulation of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: 21st International Microprocesses and Nanotechnology Conference(MNC 2008)
  • Place of Presentation: JAL Resort Sea Hawk Hotel, Fukuoka, Japan
  • Year and Date: 2008-10-29
• [Presentation] Visualization of biological macromolecular nanostructures by eclectron cryomicroscopy. (2008)
  • Author(s): Namba, K.
  • Organizer: The 4th Workshop of the UK-Japan Bionanotechnology Collaboration
  • Place of Presentation: St. Catherine's College (Oxford) Kobe Institute, Japan
  • Year and Date: 2008-09-17
• [Presentation] Molecular mechanisms of self-assembly and mechanical functions of the bacterial flagellum. (2008)
  • Author(s): Namba, K.
  • Organizer: Berzelius days
  • Place of Presentation: Stockholm University, Stockholm, Sweden
  • Year and Date: 2008-01-31
• [Presentation] Molecular mechanisms of self-assembly and switching of the bacterial flagellum. (2007)
  • Author(s): Namba, K.
  • Organizer: 2007 MRS Fall Meeting
  • Place of Presentation: Sheraton Boston Hotel, Boston
  • Year and Date: 20071106-20071130
• [Presentation] Molecular mechanisms of self-assembly and conformational switching of the bacterial flagellum for motility. (2007)
  • Author(s): Namba, K.
  • Organizer: ComBio2007 meeting
  • Place of Presentation: Sydney, Australia
  • Year and Date: 20070922-20070927
• [Presentation] Structural analysis of DNA tetrahedron. (2007)
  • Author(s): Kato, T., Goodman, R., Berry, R., Turberfild, A., Namba, K.
  • Organizer: Gordon Research Conference, Visualizing Biological Processes With 3DEM, Three Dimensional Electron Microscopy
  • Place of Presentation: New London, NH, USA
  • Year and Date: 20070624-20070629
• [Presentation] Biological macromolecular assemblies. (2007)
  • Author(s): Namba, K.
  • Organizer: Gregori Aminoff nanoscience symposium
  • Place of Presentation: Sweden
  • Year and Date: 20070613-20070614
• [Presentation] Protein export and ordered self-assembly for formation of the bacterial flagellum. (2007)
  • Author(s): Namba, K.
  • Organizer: International Workshop on Molecular Machines in Protein Folding and Protein Transport
  • Place of Presentation: Munich, Germany
  • Year and Date: 20070523-20070525
• [Presentation] Molecular mechanisms of self-assembly, size control and switching of the bacterial flagellum. (2007)
  • Author(s): Namba, K.
  • Organizer: The VII European Symposium of the Protein Society 2007
  • Place of Presentation: Stockholm University, Sweden
  • Year and Date: 20070512-20070519
• [Presentation] Molecular mechanisms of swimming and tumbling in bacterial motility. (2006)
  • Author(s): Namba, K.
  • Organizer: 16th International Toki Conference Advanced Imaging and Plasma Diagnostics
  • Place of Presentation: セラトピア土岐
  • Year and Date: 20061205-20061208
• [Presentation] Molecular mechanisms of swimming and tumbling in bacterial motility. (2006)
  • Author(s): Namba, K.
  • Organizer: 60th Annual Meeting and Symposium of the Society of General Physiologists
  • Place of Presentation: Marine Biological Laboratory, Massachusetts, USA
  • Year and Date: 20060906-20090910
• [Presentation] Molecular Mechanisms of self-assembly and switching of the bacterial flagellum. (2006)
  • Author(s): Namba, K.
  • Organizer: The 16th International Microscopy Congress "Microscopy for the 21st Century"
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 20060903-20060908
• [Presentation] Molecular mechanisms of self-assembly and switching of the bacterial flagellum. (2006)
  • Author(s): Namba, K.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Cambridge, UK
  • Year and Date: 20060817-20060823
• [Presentation] Molecular mechanisms of swimming and tumbling in bacterial motelity. (2006)
  • Author(s): Namba, K.
  • Organizer: 38th Cource-Structure and Function of Large Molecular Assemblies
  • Place of Presentation: Ettore Majorana Centenary, Erice, Italy
  • Year and Date: 20060612-20060617
• [Presentation] Molecular mechanisms of swimming and tumbling in bacterial motility. (2006)
  • Author(s): Namba, K.
  • Organizer: Hybrid Methods Meeting. "Assemblies by Hybrid Methods Symposium"
  • Place of Presentation: Lake Tahoe, CA, USA
  • Year and Date: 20060315-20060319
• [Presentation] Molecular mechanisms of bacterial swimming and tumbling in bacterial motility. (2006)
  • Author(s): Namba, K.
  • Organizer: Biophysical Society 50th Annual Meeting
  • Place of Presentation: Salt Lake City, Utah, USA
  • Year and Date: 20060218-20060222
• [Presentation] Structure mechanism of flagellar assembly regulation and switching. (2006)
  • Author(s): Namba, K.
  • Organizer: Gordon Research Conference on Sensory Transduction in Microorganisms
  • Place of Presentation: Ventura, USA
  • Year and Date: 20060122-20060128
• [Presentation] Molecular mechanisms of bacterial swimming and tumbling. (2005)
  • Author(s): Namba, K.
  • Organizer: 15th IUPAB/ 5th EBSA Meeting
  • Place of Presentation: Montpellier, France
  • Year and Date: 20050829-20050904
• [Presentation] Self-assembly and switching of the bacterial flagellum. (2005)
  • Author(s): Namba, K.
  • Organizer: 30th FEBS Congress & 9th IUBMB Congress
  • Place of Presentation: Eotvos University, Budapest, Hungary
  • Year and Date: 20050602-20050607
• [Presentation] Molecular mechanisms of bacterial swimming and tumbling. (2005)
  • Author(s): Namba, K.
  • Organizer: 「Frontiers of Science and Technology of Nanomaterials」ハイブリッドナノ材料国際シンポジウム
  • Place of Presentation: 東北大学・多元物質科学研究所
  • Year and Date: 2005-10-06
• [Presentation] High resolution 3D imaging of dynamic molecular. (2005)
  • Author(s): Namba, K.
  • Organizer: GRC on Three Dimensional Electron Microscopy, Colby-Sawyer College
  • Place of Presentation: New London, USA
  • Year and Date: 2005-06-12
• [Presentation] Molecular mechanisms of bacterial swimming and tumbling. (2005)
  • Author(s): Namba, K.
  • Organizer: NIH Symposium "Structural Analysis of Large Assemblies: Sizing Up to the challenges " National Institute of Health
  • Place of Presentation: Maryland, USA
  • Year and Date: 2005-06-02
• [Presentation] Molelcular mechanism of bacterial swimming and tumbling. (2004)
  • Author(s): Namba, K.
  • Organizer: 1ST Functionality of Organized Nanostructures (FON'4)
  • Place of Presentation: International Congress Center Tsukuba
  • Year and Date: 20041130-20041202
• [Book] "自己組織化体としての生体超分子", バイオナノプロセス-溶液中でナノ構造を作るウェット・ナノテクノロジーの薦め-」 (2008)
  • Author(s): 南野徹, 難波啓一
  • Total Pages: 305-317
  • Publisher: シーエムシー出版
• [Book] 4-2タンパク質でできた極小スクリュー細菌べん毛の分子機構, 最新分子マシンナノで働く"高度な機械"を目指して (2008)
  • Author(s): 今田勝巳
  • Total Pages: 104-112
  • Publisher: 化学同人
• [Book] 超分子構造アセンブリーとタンパク質ネットワークタンパク質-タンパク質相互作用. タンパク質科学構 造・物性・機能. (後藤祐児・桑島邦博・谷澤克行【編】) (2005)
  • Author(s): 今田勝巳, 難波啓一
  • Total Pages: 115-130
  • Publisher: 化学同人
• [Book] β-Helix is a likely core structure of yeast prion Sup35 amyloid fibers. (2005)
  • Author(s): Kishimoto, A., Hasegawa, K., Namba, K.
  • Total Pages: 28-29
  • Publisher: Spring8 Research Frontiers
• [Book] Mobile Structures: Cilia and Flagella.(The Encyclopedia of Molecular Cell Biology and Molecular Medicine) (2004)
  • Author(s): Yonekura, K., Namba, K.
  • Total Pages: 3-22
  • Publisher: WILEY-VCH V erlag, GmbH & Co. KGaA
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jp/labo/02