2009 Fiscal Year Final Research Report
The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.
| Project/Area Number |
16087208
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | University of Hyogo |
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Principal Investigator |
YOSHIKAWA Shinya University of Hyogo, 大学院・生命理学研究科, 教授 (40068119)
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| Co-Investigator(Kenkyū-buntansha) |
MURAMOTO Kazumasa 兵庫県立大学, 大学院・生命理学研究科, 准教授 (50305679)
ITOH Kyoko 兵庫県立大学, 大学院・生命理学研究科, 助教 (70206316)
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| Project Period (FY) |
2004 – 2009
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| Keywords | 酸素反応機構 / 膜蛋白質 / ミトコンドリア / エネルギー変換 / X線結晶構造解 / チトクロム酸化酵素 / NADH-ユビキノン還元酵素 |
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| Research Abstract |
Complete structures of the intrinsic lipids in bovine cytochrome c oxidase were determined. His503 couples the proton-pumping process with O_2 reduction process. Resting oxidized enzyme has a peroxide bridged in the O_2 reduction site. The bound O_2^- at Fe_<a3>^<2+> is completely reduced by a non-consecutive three electron donation. The water-channel of the H-channel blocks the reverse proton leak to provide the highly effective energy coupling. Two dimensional crystals of NADH-ubiquinone reductase and F0F1ATPase were obtained. Highly sensitive infrared apparatus applicable for any protein system in aqueous solution was constructed.
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Research Products
(45 results)
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[Journal Article] Red-Excitation Resonance Raman Analysis of the nFe=O Mode of Ferryl-Oxo Hemoproteins2008
Author(s)
Ikemura, K., Mukai, M., Shimada, H., Tsukihara, T., Yamaguchi, S., Shinzawa-Itoh, K., Yoshikawa, S., Ogura, T.
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Journal Title
J. Am. Chem. Soc. 130
Pages: 14384-14385
Peer Reviewed
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[Journal Article] Resonance Raman spectra of the FMN of the bovine heart NADH: coenzyme Q oxidoreductase, the largest membrane protein in the mitochondrial respiratory system2007
Author(s)
Sugiyama, H., Nakatsubo, R., Yamaguchi, S., Ogura, T., Shinzawa-Itoh, K. Yoshikawa, S.
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Journal Title
J. Bioenrg. Biomembr. 39
Pages: 145-148
Peer Reviewed
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[Journal Article] A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase2007
Author(s)
Muramoto, K., Hirata, K., Shinzawa-Itoh, K., Yoko-o, S., Yamashita, E., Aoyama, H., Tsukihara, T., Yoshikawa, S.
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Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 7881-7886
Peer Reviewed
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[Journal Article] Structures and physiological roles of thirteen integral lipids of bovine heart cytochrome c oxidase2007
Author(s)
K. Shinzawa-Itoh, H. Aoyama, K. Muramoto, H. Terada, T. Kurauchi, Y. Tadehara, A. Yamasaki, T. Sugimura, S. Kurono, K. Tsujimoto, T. Mizushima, E. Yamashita, T. Tsukihara, S. Yoshikawa
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Journal Title
EMBO J. 26
Pages: 1713-1725
Peer Reviewed
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[Presentation] Structural analyses for lipid/protein interactions in bovine heart cytochrome c oxidase2008
Author(s)
Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, Tadehara Y, Yamasaki A, Sugimura T, Kurono S, Tsujimoto K, Mizushima T, Yamashita E, Tsukihara T, Yoshikawa S.
Organizer
The 15th European Bioenergetics Conference
Place of Presentation
Dublin, Ireland
Year and Date
20080719-20080724
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