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2008 Fiscal Year Final Research Report

Exhaustive molecular genetic analysis and molecular ecology database construction of tree and ectomycorrhizal fungal species

Research Project

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Project/Area Number 16101008
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Resource conservation science
Research InstitutionThe University of Tokyo

Principal Investigator

HOGETSU Taizo  The University of Tokyo, 大学院・農学生命科学研究科, 教授 (10107170)

Co-Investigator(Kenkyū-buntansha) LIAN Chunlan  東京大学, アジア生物資源環境研究センター, 准教授 (40376695)
GOTO Susumu  東京大学, 大学院・農学生命科学研究科, 准教授 (60323474)
NARA Kazuhide  東京大学, アジア生物資源環境研究センター, 助教 (60270899)
Co-Investigator(Renkei-kenkyūsha) 東京大学, アジア生物資源環境研究センター, 准教授 (40376695)
GOTO Susumu  東京大学, 大学院・農学生命科学研究科, 准教授 (60323474)
NARA Kazuhide  東京大学, アジア生物資源環境研究センター, 助教 (60270899)
Project Period (FY) 2004 – 2008
Keywordsマイクロサテライト / SSR / 葉緑体DNA / 繁殖生態 / 遺伝地理 / 共生菌類
Research Abstract

(1)分子生態学解析に必要不可欠な核マイクロサテライトマーカーの新たな作製方法を確立した。確立した方法を用いて、多数の植物種、外生菌根菌種のマーカーを作製した。
(2)マイクロサテライトマーカーを用いた分子生態学解析により、北方林樹木やマングローブ林樹木など多様な植物種の多様な繁殖様式を明らかにした。
(3)森林の主要な共生菌類である外生菌根菌について、核マイクロサテライトマーカー等による分子生態学解析を行い、通常私たちの目に触れることのない森林の地下での繁殖特性を明らかにした。
(4)葉緑体マイクロサテライトマーカーを用いて、マングローブ林樹木などいくつかの樹種で、広い地域での遺伝地理的分化を明らかにした。
(5)本研究では、新たなマーカーの塩基配列や繁殖特性など、大量の森林樹木および外生菌根菌に関する分子生態情報を蓄積した。この蓄積は、今後、将来世代のための分子生態データベース構築への取り組みに弾みを付けるはずである。

  • Research Products

    (30 results)

All 2010 2009 2008 2007 2006 2005 2004

All Journal Article (11 results) Presentation (12 results) Book (7 results)

  • [Journal Article] Development of 12 microsatellite markers in Euptelea polyandra by a random tailed genome-walking method using Phi29 DNA polymerase2009

    • Author(s)
      Wu B, Kurokochi H, Hogetsu T
    • Journal Title

      Conservation Genetics Resources 1

      Pages: 59-61

  • [Journal Article] Nuclear and chloroplast microsatellite analysis of Abies sachalinensis regeneration on fallen logs in a sub-boreal forest in Hokkaido2008

    • Author(s)
      Lian C, Goto S, Kubo T, Takahashi Y, Nakagawa M, Hogetsu T
    • Journal Title

      Japan. Molecular Ecology 17

      Pages: 2948-2962

  • [Journal Article] Mating system, pollen and propagule dispersal, and spatial genetic structure in a high-density population of the mangrove tree Kandelia candel2008

    • Author(s)
      Geng Q, Lian CL, Goto S, Tao J, Kimura M, Islam MS, Hogetsu T
    • Journal Title

      Molecular Ecology 17

      Pages: 4724-4739

  • [Journal Article] Genetic structure of populations of the pinewood nematode Bursaphelenchus xylophilus, the pathogen of pine wilt disease, between and within pine forests2007

    • Author(s)
      Zhou Z, Wu BY, Sakaue D, Hogetsu T
    • Journal Title

      Phytopathology 97

      Pages: 304-310

  • [Journal Article] Element biofortification : can mycorrhizas potentially offer a more effective and sustainable pathway to curb human malnutrition?2007

    • Author(s)
      He, X.H. Nara, K.
    • Journal Title

      Trends in plant science 12

      Pages: 331-333

  • [Journal Article] An improved technique for isolating codominant compound microsatellite markers2006

    • Author(s)
      Lian CL, Gueng QF, Wadud MA, Shimatani k, Hogetsu T
    • Journal Title

      Journal of Plant Research 119

      Pages: 417-419

  • [Journal Article] Fat-tailed gene flow in the dioecious canopy tree species, Fraxinus mandshurica var. japonica revealed by microsatellites2006

    • Author(s)
      Goto S, Shimatani K, Yoshimaru H, Takahashi Y
    • Journal Title

      Molecular Ecology 15

      Pages: 2985-2996

  • [Journal Article] Tricholoma matsutake in a natural Pinus densiflora forest : correspondence between above- and below-ground genets, association with multiple host trees and alteration of existing ectomycorrhizal communities2006

    • Author(s)
      Lian CL, Narimatsu M, Nara K, Hogetsu T
    • Journal Title

      New Phytologist 171

      Pages: 825-836

  • [Journal Article] Genetic structure of Cenococcum geophilum populations in an early successional volcanic desert on Mount Fuji as revealed by microsatellite markers2005

    • Author(s)
      Wu B, Nara K, Hogetsu T
    • Journal Title

      New Phytologist 165

      Pages: 285-293

  • [Journal Article] Ectomycorrhizal fungi on established shrubs facilitate subsequent seedling establishment of successional plant species2004

    • Author(s)
      Nara K, Hogetsu T
    • Journal Title

      Ecology 85

      Pages: 1700-1707

  • [Journal Article] High somatic instability of a microsatellite locus in a clonal tree, Robinia pseudoacacia2004

    • Author(s)
      Lian C, Oishi R, Miyashita N, Hogetsu T
    • Journal Title

      Theoretical and Applied Genetics 108

      Pages: 836-841

  • [Presentation] マイクロサテライトマーカーを用いたマングローブの集団遺伝的構造と分散動態2010

    • Author(s)
      練春蘭
    • Organizer
      共同利用研究集会「沿岸海洋生物の広域動態研究の最前線」
    • Place of Presentation
      東京大学海洋研究所
    • Year and Date
      20100000
  • [Presentation] 外生菌根菌ネットワークの構造と機能2010

    • Author(s)
      宝月岱造
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      東京 (特別講演)
    • Year and Date
      2010-05-21
  • [Presentation] Biogeography of ectomycorrhizal fungi found in a dipterocarp forest in Indonesia2009

    • Author(s)
      Nara K
    • Organizer
      Asian Mycological Congress
    • Place of Presentation
      Taiwan
    • Year and Date
      20090000
  • [Presentation] Host effects on ectomycorrhizal fungal communities2008

    • Author(s)
      Nara K
    • Organizer
      21st New Phytologist Symposium "The ecology of ectomycorrhizal fungi"
    • Place of Presentation
      Montepellier France
    • Year and Date
      20080000
  • [Presentation] Germination and infectivity of ectomycorrhizal fungal spores in relation to their ecological traits during primary succession2008

    • Author(s)
      Ishida TA, Nara K, Tanaka M, Kinoshita A, Hogetsu T
    • Organizer
      21st New Phytologist Symposium "The ecology of ectomycorrhizal fungi"
    • Place of Presentation
      Montepellier, France
    • Year and Date
      20080000
  • [Presentation] Primary process of tree establishment in vegetation patches on gravel areas at sediment dams2008

    • Author(s)
      Kurokochi H, Matsushita N, Hogetsu T
    • Organizer
      The 10th International Congress of Ecology (INTECOL)
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      20080000
  • [Presentation] 多摩川河川敷に生育するニセアカシアの遺伝的構造と花粉の散布様式2007

    • Author(s)
      練春蘭
    • Organizer
      外来種ニセアカシアシンポジウム
    • Place of Presentation
      信州大
    • Year and Date
      20070000
  • [Presentation] 富士山火山荒原の菌根共生2006

    • Author(s)
      奈良一秀
    • Organizer
      第12回微生物をめぐる生物問相互作用に関する研究集会
    • Place of Presentation
      東京大学・11月
    • Year and Date
      20060000
  • [Presentation] Functional ecology of ectomycorrhizal symbiosis during early primary succession2006

    • Author(s)
      Nara K
    • Organizer
      The 5th International Conference on Mycorrhiza
    • Place of Presentation
      Granada, Spain
    • Year and Date
      20060000
  • [Presentation] Ectomycorrhizal symbioses and vegetation development in the primary successional volcanic desert on Mount Fuji2006

    • Author(s)
      Nara K
    • Organizer
      The 8th International Mycological Congress
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      20060000
  • [Presentation] Ectomycorrhizal fungal networks facilitate plant succession2006

    • Author(s)
      Nara K
    • Organizer
      British Mycological Society Annual Conservation & Taxonomy Meeting in partnership with The Royal Botanic Gardens, Kew
    • Place of Presentation
      London, UK
    • Year and Date
      20060000
  • [Presentation] Genetic assessment of orchard crops in Japanese Black Pine clonal seed orchard consisting of pinewood nematode-resistant clones2004

    • Author(s)
      Goto S, Miyahara F, Mori Y, Watanabe A
    • Organizer
      Proceeding of 2004 IUFRO Joint Conference Division 2, Forest Genetics and Tree breeding in the age of genomics : progress and future
    • Year and Date
      20040000
  • [Book] マイクロサテライトマーカーが明かすニセアカシアの繁殖特性. 崎尾均編, ニセアカシアの生態学2009

    • Author(s)
      練春蘭・木村恵・崎尾均・宝月岱造
    • Total Pages
      185-200
    • Publisher
      文一総合出版
  • [Book] ジーンフロー研究の成果をどのように森林管理に活かすか? 北方林業会編, 北の森づくりQ&A(北方林業会創立60周年記念誌)2009

    • Author(s)
      後藤晋
    • Total Pages
      82-85
  • [Book] 菌根菌による植生遷移促進機構. In : 植生遷移の自然史重定・露崎編2008

    • Author(s)
      奈良一秀
    • Total Pages
      95-111
    • Publisher
      北海道大学出版会
  • [Book] Community development patterns and ecological functions of ectomycorrhizal fungi : implication from primary succession In Mycorrhiza (3rd edition), Ajit Varma ed2008

    • Author(s)
      Nara, K.
    • Publisher
      Springer-Verlag
  • [Book] Quantification of nitrogen transfer between plants linked by mycorrhizal networks In : Mycorrhizal Manual (2nd edition), Ajit Varma ed.2008

    • Author(s)
      He, X. H., Critchley, C., Nara, K., Southworth, D., Bledsoe, C. S.
    • Publisher
      Springer-Verlaa
  • [Book] ヤチダモ・アオダモ, 北海道林木育種協会編 北海道における林木育種と森林遺伝資源2008

    • Author(s)
      後藤晋
    • Total Pages
      181-188
  • [Book] 共生系としての森林鈴木和夫編著「森林保護学」2004

    • Author(s)
      宝月岱造
    • Total Pages
      49-57

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Published: 2011-06-18   Modified: 2016-04-21  

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