Regulation of nucleocytoplasmic protein transport and nuclear stress response

2008 Fiscal Year Final Research Report

• Project/Area Number: 16107004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YONEDA Yoshihiro Osaka University, 生命機能研究科, 教授 (80191667)
• Co-Investigator(Renkei-kenkyūsha): SEKIMOTO Toshihiro 大阪大学, 医学系研究科, 助教 (20305634)
• Project Period (FY): 2004 – 2008
• Keywords: 細胞核 / ストレス応答 / 核蛋白質輸送 / 核輸送因子 / importin

Research Abstract

核-細胞質間蛋白質輸送に関する研究は、輸送因子や核膜孔複合体構成因子の同定・性状解析などが進み、基本メカニズムの理解が進んだ。しかし、核-細胞質間蛋白質輸送が、細胞分化、個体発生、細胞周期などの複雑な生命現象にどのように関連しているか不明である。本研究では、細胞核が様々なストレスにどのように応答するのかを、核-細胞質間蛋白質輸送制御の観点から解明を進めた。

Research Products

• [Journal Article] Triggering neural differentiation of ES cells by subtype switching of importin-α (2007)
  • Author(s): Yasuhara, N., Shibazaki, N., Tanaka, S., Nagai, M., Kamikawa, Y., Oe, S., Kamachi, Y., Kondoh, H. and Yoneda, Y
  • Journal Title: Nature Cell Biol 9 Pages: 72-79
  • Peer Reviewed
• [Journal Article] Arx1 functions as an unorthodox nuclear export receptor for the 60S pre-ribosomal subunit (2007)
  • Author(s): Bradatsch, B., Katahira, J., Kowalinski, E., Bange, G., Yao, W., Sekimoto, T., Baumgartel, V., Boese, G., Bassler, J., Wild, K., Peters, R., Yoneda, Y., Sinning, I. and Hurt, E
  • Journal Title: Mol. Cell 27 Pages: 767-779
  • Peer Reviewed
• [Journal Article] Mechanism of the stress-induced collapse of the Ran distribution (2006)
  • Author(s): Yasuda, Y., Miyamoto, Y., Saiwaki, T. and Yoneda, Y
  • Journal Title: Exp. Cell Res 312 Pages: 512-520
  • Peer Reviewed
• [Journal Article] The 70 kDa heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors (2005)
  • Author(s): Kose, S., Furuta, M., Koike, M., Yoneda, Y. and Imamoto, N
  • Journal Title: J. Cell Biol 171 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Importin α transports Ca^<2+>/calmodulin-dependent protein kinase type IV (CaMKIV) to the nucleus without utilizing importin β (2005)
  • Author(s): Kotera, I., Sekimoto, T., Miyamoto, Y., Saiwaki, T., Nagoshi, E., Sakagami, H., Kondo, H. and Yoneda, Y
  • Journal Title: EMBO J 24 Pages: 942-951
  • Peer Reviewed
• [Journal Article] Cellular stresses induce the nuclear accumulation of importin α and cause a conventional nuclear import block (2004)
  • Author(s): Miyamoto, Y., Saiwaki, T., Yamashita, J., Yasuda, Y., Kotera, I., Shibata, S., Shigeta, M., Hiraoka, Y., Haraguchi, T., and Yoneda, Y
  • Journal Title: J. Cell Biol 165 Pages: 617-623
  • Peer Reviewed
• [Presentation] Kate Loveland「精子細胞形成過程におけるimportinαの機能解析」BMB2008 (2009)
  • Author(s): 宮本洋一、安田善也、山城朋子、永井理博、米田悦啓、David Jans
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20091209-12
• [Presentation] Functional analysis of nuclear importin alpha accumulating in response to the stress (2008)
  • Author(s): Yasuda, Y., Miyamoto, Y. and Yoneda, Y
  • Organizer: Cold Spring Harbor Laboratory Meeting on Dynamic Organization of Nuclear Function
  • Place of Presentation: New York
  • Year and Date: 20080917-21
• [Presentation] Nuclear protein import and its significance to cell function (2008)
  • Author(s): Yoneda,Y
  • Organizer: 2^<nd> International SOX Meeting
  • Place of Presentation: Hyogo JAPAN
  • Year and Date: 20080916-19
• [Presentation] Nucleocytoplasmic transport of proteins and RNAs and its significance to cell function (2008)
  • Author(s): Yoneda, Y., Katahira, J., Sekimoto, T., Yasuhara, N., and Oka, M.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Nuclear accumulation of importinα suppresses DNA replication (2008)
  • Author(s): Mizuguchi, C., Yasuda, Y. and Yoneda, Y
  • Organizer: 第60回日本生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-0701
• [Presentation] Importin alpha has multiple functions (2007)
  • Author(s): Yoneda, Y
  • Organizer: EMBO Workshop on Mechanisms of Nucleocytoplasmic Transport
  • Place of Presentation: Taormina, Sicily, Italy
  • Year and Date: 20071027-31
• [Presentation] 核-細胞質間分子流通機構と生命機能の調節 (2007)
  • Author(s): 米田悦啓
  • Organizer: 第71回日本生化学会中部支部会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2007-05-19
• [Presentation] Nucleocytoplasmic transport and its signifivance to nuclear function (2006)
  • Author(s): Yoneda, Y
  • Organizer: 20^<th> IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 20060618-23
• [Book] 分子生物学イラストレイテッド第3版 (2009)
  • Author(s): 浅利宗弘、米田悦啓
  • Total Pages: 348
  • Publisher: 羊土社
• [Book] 標準細胞生物学 (2008)
  • Author(s): 片平じゅん、米田悦啓
  • Total Pages: 358
  • Publisher: 医学書院
• [Book] Nuclear Dynamics (2007)
  • Author(s): Saiwaki, T. and Yoneda, Y
  • Total Pages: 279
  • Publisher: Springer-Verlag Inc
• [Book] 細胞核の世界-ダイナミクスから病態まで (2007)
  • Author(s): 米田悦啓、他
  • Total Pages: 383
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/