Postgenomics of plant pathogenic microorganisms that live intracellularly

2008 Fiscal Year Final Research Report

• Project/Area Number: 16108001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: NAMBA Shigetou The University of Tokyo, 大学院・農学生命科学研究科, 教授 (50189221)
• Co-Investigator(Kenkyū-buntansha): 石川 幸男 東京大学, 大学院・農学生命科学研究科, 准教 (60125987)
• Project Period (FY): 2004 – 2008
• Keywords: 細胞内寄生 / ポストゲノム / 宿主特異性 / 病原性

Research Abstract

細胞内寄生性植物病原細菌の一種であるファイトプラズマをターゲットとし、そのゲノム配列にもとづいたポストゲノミクス解析を多方面から行った。その結果、ファイトプラズマの宿主特異性を決定している分子メカニズムの解明に成功し、またファイトプラズマが持つ新規な病原性因子の単離にも成功した。

Research Products

• [Journal Article] A unique virulence factor for proliferationand dwarfism in plants identified from aphytopathogenic bacterium (2009)
  • Author(s): Hoshi, A., Oshima, K., Kakizawa, S., Ishii, Y.,Ozeki, J., Hashimoto, M., Komatsu, K, Kagiwada, S., Yamaji, Y.& Namba, S.
  • Journal Title: Proc. Natl. Acad.Sci. USA 106 Pages: 6416-6421
  • Peer Reviewed
• [Journal Article] of Onion Yellowsphytoplasma (OY-NIM), the plasmid encodedtransmembrane protein ORF3 lacks the majorpromoter region (2009)
  • Author(s): Ishii, Y., Kakizawa, S., Hoshi, A., Maejima, K., Kagiwada, S., Yamaji, Y., Oshima, K.& Namba, S.
  • Journal Title: Microbiology-(UK) (in press)
  • Peer Reviewed
• [Journal Article] Cloning of immunodominantmembrane protein genes ofphytoplasmas and their in planta expression (2009)
  • Author(s): Kakizawa, S., Oshima, K., Ishii, Y., Hoshi, A., Maejima, K., Jung, H. Y., Yamaji, Y. & Namba, S
  • Journal Title: FEMS Microbiol. Lett 293 Pages: 92-101
  • Peer Reviewed
• [Journal Article] Heterogeneicdynamics of the structures of multiple geneclusters in two pathogenetically different linesoriginating from the same phytoplasma (2008)
  • Author(s): Arashida, R., Kakizawa, S., Hoshi, A., Ishii, Y., Jung, H. Y., Kagiwada, S., Yamaji, Y., Oshima,K.& Namba, S
  • Journal Title: DNA Cell Biol 27 Pages: 209-217
  • Peer Reviewed
• [Journal Article] Cloning andcharacterization of the antigenic membraneprotein (Amp) gene and in situ detection ofAmp from malformed flowers infected withJapanese hydrangea phyllody phytoplasma (2008)
  • Author(s): Arashida, R., Kakizawa, S., Ishii, Y., Hoshi, A., Jung, H. Y., Kagiwada, S., Yamaji, Y., Oshima,K.& Namba, S
  • Journal Title: Phytopathology 98 Pages: 769-775
• [Journal Article] Phytoplasmas : Bacteria that manipulate plants and insects (2008)
  • Author(s): Hogenhout, S. A., Oshima, K., Ammar, E.-D., Kakizawa, S., Kingdom, H. N.& Namba, S.
  • Journal Title: Mol. Plant Pathol 9 Pages: 403-423
• [Journal Article] Presence of two glycolyticgene clusters in a severe pathogenic line ofCandidatus Phytoplasma asteris (2007)
  • Author(s): Oshima, K., Kakizawa, S., Arashida, R., Ishii,Y., Hoshi, A., Hayashi, Y, Kagiwada, S.& Namba, S.
  • Journal Title: Mol. Plant Pathol 8 Pages: 481-489
• [Journal Article] Positive selection acting on a surfacemembrane protein of the plant-pathogenicphytoplasmas (2006)
  • Author(s): Kakizawa, S., Oshima, K., Jung, H. Y., Suzuki,S., Nishigawa, H., Arashida, R., Miyata, S.,Ugaki, M., Kishino, H.& Namba, S.
  • Journal Title: J. Bacteriol 188 Pages: 3424-3428
• [Journal Article] Interaction between the membraneprotein of a pathogen and insectmicrofilament complex determines insectvectorspecificity (2006)
  • Author(s): Suzuki, S., Oshima, K., Kakizawa, S., Arashida, R., Jung, H. Y., Yamaji, Y., Nishigawa, H., Ugaki, M.& Namba, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 4252-4257
• [Journal Article] Diversity and functional importance of phytoplasma membrane proteins (2006)
  • Author(s): Kakizawa, S., Oshima, K.& Namba, S.
  • Journal Title: Trends Microbiol 14 Pages: 254-256
• [Journal Article] Detection of "CandidatusPhytoplasma asteris" associated with henonbamboo witches' broom in Korea (2006)
  • Author(s): Jung, H. Y., Chang, M. U., Lee, J.T.& Namba, S.
  • Journal Title: J. Gen.Plant Pathol 72 Pages: 261-263
• [Journal Article] Detection of chill injuries in the pupae of theonion maggot, Delia antiqua (Diptera : Anthomyiidae). Appl (2005)
  • Author(s): Kayukawa, T.& Ishikawa, Y.
  • Journal Title: Entomol. Zool 40 Pages: 193-198
• [Journal Article] DaTrypsin, a novel clip-domain serineproteinase gene up-regulated during winterand summer diapauses of the onion maggot (2005)
  • Author(s): Chen, B., Kayukawa, T., Jiang, H., Monteiro,A., Hoshizaki, S.& Ishikawa, Y.
  • Journal Title: Delia antiqua Gene 347 Pages: 115-123
• [Journal Article] Ostrinia scapulalis and O. zealis (2005)
  • Author(s): Tabata, J.& Ishikawa, Y.
  • Journal Title: J. Chem. Ecol 31 Pages: 1111-1124
• [Journal Article] Ovipositiondeterrents from the egg masses of adzuki beanborer,Ostrinia scapulalis and Asian corn borer (2005)
  • Author(s): Li, G.& Ishikawa, Y.
  • Journal Title: O. furnacalis. Entomol. Exp. Appl 115 Pages: 401-407
  • Peer Reviewed
• [Journal Article] Sex pheromonebiosynthesis in Ostrinia zaguliaevi, acongener of the European corn borer moth O.nubilalis (2005)
  • Author(s): Fu, X., Fukuzawa, M., Tabata, J., Tatsuki, S.& Ishikawa, Y.
  • Journal Title: Insect Biochem. Mol. Biol 36 Pages: 621-626
  • Peer Reviewed
• [Journal Article] Transinfection reveals the crucialimportance of Wolbachia genotypes indetermining the type of reproductivealteration in the host (2005)
  • Author(s): Sakamoto, H., Ishikawa, Y., Sasaki, T.,Kikuyama, S., Tatsuki, S.& Hoshizaki, S.
  • Journal Title: Genet. Res 85 Pages: 205-210
  • Peer Reviewed
• [Journal Article] Expression of mRNA for thet-complex polypeptide-1, a subunit ofchaperonin CCT, is upregulated in associationwith increased cold hardiness in Deliaantiqua (2005)
  • Author(s): Kayukawa, T., Chen, B., Miyazaki, S.,Itoyama, K., Shinoda, T.& Ishikawa, Y.
  • Journal Title: Cell Stress Chaperones 10 Pages: 204-210
  • Peer Reviewed
• [Journal Article] Feedingglucose or sucrose, but not trehalose,suppresses the starvation-induced prematurepupation in the yellow-spotted longicornbeetle, Psacothea hilaris (2005)
  • Author(s): Munyiri, F.N.& Ishikawa, Y.
  • Journal Title: J. Insect Physiol 51 Pages: 1005-1012
  • Peer Reviewed
• [Journal Article] The expression of HSP90gene in response to winter and summerdiapauses and thermal-stress in the onionmaggot (2005)
  • Author(s): Chen, B., Kayukawa, T., Monteiro, A.& Ishikawa, Y.
  • Journal Title: Delia antiqua. Insect Mol. Biol 14 Pages: 697-702
  • Peer Reviewed
• [Journal Article] Secretion of immunodominant membraneprotein from onion yellows phytoplasmathrough the Sec protein-translocation systemin Escherichia coli (2004)
  • Author(s): Kakizawa, S., Oshima, K., Nishigawa, H., Jung, H. Y., Wei, W., Suzuki, S., Tanaka, M., Miyata, S., Ugaki, M.& Namba, S.
  • Journal Title: Microbiology 150 Pages: 135-142
  • Peer Reviewed
• [Journal Article] An antibody against the SecAmembrane protein of one phytoplasma reactswith those of phylogenetically differentphytoplasmas (2004)
  • Author(s): Wei, W., Kakizawa, S., Jung, H.Y., Suzuki, S.,Tanaka, M., Nishigawa, H., Miyata, S.,Oshima, K., Ugaki, M., Hibi, T.& Namba, S.
  • Journal Title: Phytopathology 94 Pages: 683-686
  • Peer Reviewed
• [Journal Article] Inplanta dynamic analysis of onion yellowsphytoplasma using localized inoculation byinsect transmission (2004)
  • Author(s): Wei, W., Kakizawa, S., Suzuki, S., Jung, H.Y., Nishigawa, H., Miyata, S., Oshima, K.,Ugaki, M., Hibi, T.& Namba, S.
  • Journal Title: Phytopathology 94 Pages: 244-250
  • Peer Reviewed
• [Journal Article] Reductive evolution suggested fromthe complete genome sequence of aplant-pathogenic phytoplasma (2004)
  • Author(s): Oshima, K., Kakizawa, S., Nishigawa, H.,Jung, H.Y., Wei, W., Suzuki, S., Arashida, R.,Nakata, D., Miyata, S., Ugaki, M.& Namba, S.
  • Journal Title: Nature Genet 36 Pages: 27-29
  • Peer Reviewed
• [Journal Article] Wolbachia infection and all-femaletrait in Ostrinia orientalis and O. zaguliaevi(Lepidoptera : Crambidae) (2004)
  • Author(s): Kageyama, D., Nishimura, G., Ohno, S.,Takanashi, T., Hoshizaki, S.& Ishikawa, Y.
  • Journal Title: Entomol. Exp.Appl 111 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Evidence for the presence of athreshold weight for entering diapause in theyellow-spotted longicorn beetle (2004)
  • Author(s): Munyiri, F.N., Shintani, Y.& Ishikawa, Y.
  • Journal Title: Psacothea hilaris. J. Insect Physiol 50 Pages: 295-301
  • Peer Reviewed
• [Presentation] Positive selection on a surface membrane protein and insect vector specificity of phytoplasma (2008)
  • Author(s): Kakizawa, S., Ishii, Y., Hoshi, A., Jung, H. Y., Oshima, K., Namba, S.
  • Organizer: InternationalCongress of Bacteriology and AppliedMicrobiology
  • Place of Presentation: Istanbul
  • Year and Date: 20080805-09
• [Presentation] Sequence diversity and positive selection of a surface membrane protein of phytoplasma (2008)
  • Author(s): Kakizawa, S., Ishii, Y., Hoshi, A., Jung, H. Y., Oshima, K., Namba, S.
  • Organizer: 17th International Congress of the International Organization for Mycoplasmology
  • Place of Presentation: 天津医科大学
  • Year and Date: 20080706-11
• [Presentation] 葉化組織におけるファイトプラズマの局在解析 (2008)
  • Author(s): 星 朱香, 石井 佳子, 川西 剛史, 柿澤 茂行, 大島 研郎, 難波 成任
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 20080530-31
• [Presentation] ファイトプラズマのプラスミド上の膜タンパク質遺伝子ORF3のプロモーター解析 (2008)
  • Author(s): 石井佳子, 星朱香, 川西剛史, 柿澤茂行, 大島研郎, 難波成任
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 20080530-31
• [Presentation] ファイトプラズマの検出・診断の歴史と現状 (2008)
  • Author(s): 柿澤茂行, 星朱香, 石井佳子, 川西剛史, 大島研郎, 難波成任
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 20080530-31
• [Presentation] マイコプラズマ様微生物の世界-ファイトプラズマ発見の歴史と新展開- (2008)
  • Author(s): 大島研郎, 石井佳子, 星朱香, 川西剛史, 柿澤茂行, 難波成任
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 20080530-31
• [Presentation] ファイトプラズマゲノムにおけるスクロースホスホリラーゼ遺伝子の機能欠損 (2008)
  • Author(s): 大島研郎, 星朱香, 石井佳子, 柿澤茂行, 難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: くにびきメッセ
  • Year and Date: 20080426-28
• [Presentation] ファイトプラズマのプラスミド上にコードされる膜タンパク質ORF3の免疫組織化学的解析 (2008)
  • Author(s): 星 朱香, 石井佳子, 柿澤茂行, 大島研郎, 難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: くにびきメッセ
  • Year and Date: 20080426-28
• [Presentation] ファイトプラズマの昆虫伝搬能喪失株におけるプラスミド上のプロモーター配列欠失について (2008)
  • Author(s): 石井佳子, 星 朱香, 柿澤茂行, 大島研郎, 難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: くにびきメッセ
  • Year and Date: 20080426-28
• [Presentation] 植物病原細菌ファイトプラズマの膜タンパク質Ampの昆虫感染への関連性 (2008)
  • Author(s): 大島研郎, 柿澤茂行, 難波成任
  • Organizer: 平成20年度日本農芸化学会大会
  • Place of Presentation: 名城大学
  • Year and Date: 20080327-29
• [Book] 感染・炎症・炎症 (2009)
  • Author(s): 柿澤茂行, 大島研郎, 難波成任
  • Total Pages: 48-51
  • Publisher: 植物病原菌ファイトプラズマの全ゲノム解読とベクターの特異性の分子機構
• [Book] ファイトプラズマによる植物の病害 (2008)
  • Author(s): 難波成任
  • Publisher: 微生物の事典朝倉書店
• [Book] In Recent ResearchDevelopments in Bioenergetics (2005)
  • Author(s): Oshima, K. and Namba, S.
  • Total Pages: 45-61
  • Publisher: Metabolicpathways encoded in the small genomes ofbacterial parasites
• [Book] 怠け者の小さな植物病原微生物 : ファイトプラズマ-初めて入れられた分子生物学的メス (2005)
  • Author(s): 柿澤茂行, 難波成任
  • Total Pages: 109(15)
  • Publisher: BRAIN テクノニュース
• [Book] Pathogenicity andStrategies for Control (ed. Alain Blanchardand Glenn Browning, Horizon Bioscience) (2005)
  • Author(s): Namba, S., Oshima, K., Gibb, K.
  • Total Pages: 1-20
  • Publisher: Phytoplasma Genomics. In Mycoplasmas : Molecular Biology
• [Book] 植物病原細菌ファイトプラズマの全ゲノム解読の意義-退行的進化を遂げた微生物の生存戦 (2004)
  • Author(s): 大島研郎, 難波成任
  • Total Pages: 649-653
  • Publisher: 蛋白質・核酸・酵素
• [Book] 怠け者の小さな植物病原微生物-生物はどこまで遺伝情報を減らすことが出来るのか (2004)
  • Author(s): 大島研郎, 難波成任
  • Total Pages: 154-160
  • Publisher: 化学と生物