2007 Fiscal Year Final Research Report Summary
Bone tissue engineering by cell aggregates
Project/Area Number |
16200032
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | The University of Tokyo |
Principal Investigator |
USHIDA Takashi The University of Tokyo, Graduate School of Medicine, Professor (50323522)
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Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Katsuko The University of Tokyo, Graduate School of Engineering, Associate Professor (90343144)
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Project Period (FY) |
2004 – 2007
|
Keywords | tissue engineered bone / elements / stem cells / transplantation / TCP / Dynamics characteristic |
Research Abstract |
For clinical operativity of bone regeneration, adequate committed osteoblasts are required. Bone marrow mesenchymal stromal cells (MSCs) could provide a source for bone regeneration. In addition, it is well known that Mesenchymal cell condensation plays an important role in the early stages of membranous osteogenesis and dynamic flow can positively influence and enhance osteodifferentiation of MSCs. Therefore, to imitating bone development, the rotation culture method was adopted in our experiments as compared with static culture. Osteogenic differentiation was evaluated by histological, immunochemical and RT-PCR analyses. We showed that the cell condensation and three-dimensional dynamic environment in rotation culture may enhance osteoblastic phenotype development and mineralized matrix synthesis in vitro. The extracellular matrix of cell aggregates was endogenously synthesized entirely naturally by the cells themselves. The cell aggregate by rotation culture showed mineral deposition and osteocalcin expression at 7 days, whereas static culture was detected at 14 days. Rotation culture is a much more closely resemble the cell environment in vivo and suitable culture environment than static culture in well plates.
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[Journal Article] Reduced platelet adhesion to titanium metal coated with apatite, album in-apatite composite or laminin-apatite composite2005
Author(s)
Masaki Uchida, Atsuo Ito, Katasuko S Furukawa, Keigo Nakamura, Yuji Onimura, Ayako Oyane, Takashi Ushida, Takashi Yamane, Tamotsu Tamaki, Tetsuya Tateishi
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Journal Title
Biomaterials 26
Pages: 6924-6931
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Reduced platelet adhesion to titanium metal coated with apatite, albumin-apatite composite or laminin-apatite composite2005
Author(s)
Masaki Uchida, Atsuo Ito, Katasuko S Furukawa, Keigo Nakamura, Yuji Onimura, Ayako Oyane, Takashi Ushida, Takashi Yamane, Tamotsu Tamaki, Tetsuya Tateishi
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Journal Title
Biomaterials "Peer reviewed" 26
Pages: 6924-6931
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 骨髄由来細胞凝集体を用いた骨再生2007
Author(s)
山崎浩平, 末永英之, 岸良健太郎, 古川克子, 牛田多加志
Organizer
第19回バイオエンジニアリング講演会
Place of Presentation
宮城
Year and Date
20070107-08
Description
「研究成果報告書概要(和文)」より
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[Presentation] Cartilage by mold technology2007
Author(s)
Katsuko S Furukawa, Tetsuya Tateishi, Takasbi Ushida
Organizer
19th Bioengineering Conference, 2004 Annual Meeting of BED/JSME
Place of Presentation
Miyagi in Japan
Year and Date
20070107-08
Description
「研究成果報告書概要(欧文)」より
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[Presentation] カーボンナノチューブの抗血栓性評価2006
Author(s)
佐藤 雄介, 古川 克子, 丸山 茂夫, 水原 和行, 立石 哲也, 牛田 多加志
Organizer
第18回日本機械学会バイオエンジニアリング講演会
Place of Presentation
新潟
Year and Date
20060114-15
Description
「研究成果報告書概要(和文)」より
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[Presentation] Antithrombotic experiment of carbon nanotube2006
Author(s)
Yusuke Sato, Katsuko S Furukawa, Sigeo Marutyama, Kazuyuki Mizuhara, Tetsuya Tateishi, Takashi Ushida
Organizer
18th Bioengineering Conference, 2004 Annual Meeting of BED/JSME
Place of Presentation
Nigata in Japan
Year and Date
20060114-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effect of Mg on surface roughness and protein content of protein- apatite composite layers2005
Author(s)
Y. Ishikawa, N. Kondo, Y. Sogo, A. Ito, E Uchimura, A Oyane, T. Ohno, A. Yamazaki, T. Ushida, K. Furukawa,
Organizer
Bioceramics 18
Place of Presentation
Kyoto in Japan
Year and Date
20051205-08
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 親媒性高分子鎖を有する表面の血液適合性評価2005
Author(s)
大矢根 綾子, 横山 英明, 関 明子, 石曽根 隆, 横山 敬郎, 古川 克子, 牛田 多加志.立石 哲也
Organizer
日本バイオマテリアル学会大会
Place of Presentation
京都
Year and Date
20051128-29
Description
「研究成果報告書概要(和文)」より
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[Presentation] Hemocompatibility of Amphipathic materials2005
Author(s)
A. Ohyane, H. Yokoyama, A. Seki, T. Ishizone, H. Yokoyama, K. S. Furukawa, T. Ushida, T. Tateishi
Organizer
The 27th Annual Meeting of Japanese Society for Biomaterials
Place of Presentation
Kyoto in Japan
Year and Date
20051128-29
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Development of micro immunoreaction-based cell sorter for regenerative medicine2005
Author(s)
Inokuchi, H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K., and Ushida, T.
Organizer
22nd Sensor Symp
Place of Presentation
Tokyo in Japan
Year and Date
20051020-21
Description
「研究成果報告書概要(和文)」より
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[Presentation] Effect of Hydrostatic Pressure on chondrocytes2005
Author(s)
Makoto Kawanishi, Katsuko S Furukawa, Takashi Ushida, Kouzo Nakamura, Tetsuya Tateishi
Organizer
20st Annual Research Meeting of the Japanese Orthopaedic Association
Place of Presentation
Mie in Japan
Year and Date
20051020-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Micro magnetic separator for stem cell sorting system, Marina del Rey2005
Author(s)
Miwa, J., Tan, W.H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K. and Ushida, T.
Organizer
1st Intl. Conf. BNI Fusion
Place of Presentation
CA, USA
Year and Date
20050720-22
Description
「研究成果報告書概要(和文)」より
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[Presentation] Micro magnetic separator for stem cell sorting system, Marina del Rey2005
Author(s)
Miwa, J., Tan, W. H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K., Ushida, T
Organizer
Ist Intl. Conf. BNI Fusion
Place of Presentation
CA, USA
Year and Date
20050720-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] LP3 Production by Mechanical Stress2005
Author(s)
T. Yshida, M. Sano, S. Iwayoshi, K. S. Furukawa, T. Ushida
Organizer
45th Annual Conference of Japanese Society for Medical and Biological Engineering
Place of Presentation
Ibaragi in Japan
Year and Date
20050425-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 細胞の接着と剥離に関する研究2005
Author(s)
下森 徹平, 古川 克子, 鈴木 雄二, 鹿園 直毅, 笠木 伸英, 牛田 多加志
Organizer
第17回日本機械学会バイオエンジニアリング講演会
Place of Presentation
愛知
Year and Date
20050122-23
Description
「研究成果報告書概要(和文)」より
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[Presentation] 血液適合性材料の評価2005
Author(s)
古川克子
Organizer
マイクロフロービジュアリゼーション研究会
Place of Presentation
東京
Year and Date
2005-01-24
Description
「研究成果報告書概要(和文)」より
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[Presentation] 表面にアパタイトを形成させたチタンの抗血栓性2004
Author(s)
内田 昌樹, 伊藤 敦夫, 中村 桂吾, 鬼村 祐治, 古川 克子, 牛田 多加志, 山根 隆志, 立石 哲也
Organizer
第26回日本バイオマテリアル学会大会
Place of Presentation
茨城
Year and Date
20041115-16
Description
「研究成果報告書概要(和文)」より
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[Presentation] Spontaneous formation of blood compatible surfaces2004
Author(s)
Ayako Ohyane, Hideaki Yokoyama, Takasi Ishizone, Masaki Uchida, Katsuko S Furukawa, Takashi Ushida, Tetsuya Tateishi
Organizer
The 26th Annual Meeting of Japanese Society for Biomaterials
Place of Presentation
Ibaragi in Japan
Year and Date
20041115-16
Description
「研究成果報告書概要(欧文)」より
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[Presentation] メカニカルストレスと細胞応答2004
Author(s)
牛田 多加志, 古川 克子, 立石 哲也
Organizer
第31回日本臨床バイオメカニクス学会
Place of Presentation
福岡
Year and Date
20041112-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] 静水圧負荷下での関節軟骨細胞の長期3次元培養2004
Author(s)
川西 誠, 田中 栄, 中村 耕三, 牛田 多加志, 古川 克子, 織田 弘美, 福林徹, 立石 哲也
Organizer
第31回日本臨床バイオメカニクス学会
Place of Presentation
福岡
Year and Date
20041112-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] Development of novel micro mixer and its application to μ-immunomagnetic cell sorter2004
Author(s)
Tan, W.-H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K., and Ushida, T.
Organizer
8th Int. Conf. Miniaturised Systems for Chemistry and Life Sciences (μTAS2004)
Place of Presentation
Malmo in Sweden
Year and Date
20040926-30
Description
「研究成果報告書概要(和文)」より
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[Presentation] Mechanical properties of tissue engineered cartilage2004
Author(s)
YU Tingchin, Katsuko S Furukawa, Shogo Miyata, Takashi Ushida, Tadaatsu Satomi, Tetsuya Tateishi
Organizer
2004 Annual Meeting of Kanto Branch Division, Japanese Society of Mechanical Engineers
Place of Presentation
Tokyo in Japan
Year and Date
20040910-11
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 剪断流れの存在下における抗血栓性材料の評価2004
Author(s)
古川 克子, 牛田 多加志, 内田 昌樹, 大島 宣雄, 玉木 保, 山根 隆志, 伊藤 敦夫, 立石 哲也
Organizer
第27回日本バイオレオロジー学会年会
Place of Presentation
東京
Year and Date
20040610-11
Description
「研究成果報告書概要(和文)」より
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[Presentation] 静水圧負荷長期培養システムの構築2004
Author(s)
大浦 敦裕, 川西 誠, 古川 克子, 牛田 多加志, 立石 哲也
Organizer
日本機械学会2004年度年次大会
Place of Presentation
北海道
Year and Date
2004-09-07
Description
「研究成果報告書概要(和文)」より
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[Book] 機械工学便覧2008
Author(s)
立石哲也, 牛田多加志, 古川克子
Publisher
(社)日本機械学会(印刷中)(掲載確定)
Description
「研究成果報告書概要(和文)」より
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