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2006 Fiscal Year Final Research Report Summary

Development of Biological Sample Separation/Extraction and Preparation Microsystems for High Performance Biosensing

Research Project

Project/Area Number 16201031
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionWaseda University

Principal Investigator

SHOJI Shuichi  Waseda University, Faculty of Science and Engineering, Professor, 理工学術院, 教授 (00171017)

Co-Investigator(Kenkyū-buntansha) FUNATSU Takashi  The University of Tokyo, Graduate school of Pharma ceutical Sciences, Professor., 大学院薬学研究科, 教授 (00190124)
HOMMA Takayuki  Waseda University, Faculty of Science and Engineering, Professor, 理工学術院, 教授 (80238823)
ARAKAWA Takahiro  Waseda University, Faculty of Science and Engineering, Research Associate, 理工学術院, 助手 (50409637)
Project Period (FY) 2004 – 2006
KeywordsMicro Fluidic Systems / Micro Total Analysis Systems / MEMS / Micro Fludic Devices / Biomolecule Sorter / Sample Injector / Glass Microstructures / Sheath Flow
Research Abstract

In recent one of the most active area of Micro Total Analysis Systems (μTAS) or Lab-on-a-Chip (LOC), DNA, Protein analysis methods and devices have been improved. However, traditional sample preparations before analysis are required in many cases. In order to realize high speed and high throughput analysis in μTAS, sample separations/extractions are requested to be obtained in microfluidic devices/systems. We applied MEMS technologies and nano technologies and fabricated micro fluidic devices and systems of nl or pl in volume.
Biomolecules sorting systems using so-gel transformation of the thermo-sensitive hydrogel as the carrier fluid were developed. High speed one inlet and two outlets sorter is realized by optimizing the channel structures and the flow behaviors. Very fast sorting within 5 msec was also realized. One inlet and 9 outlets sorting system is realized in a matrix microchannel using IR light illumination projected by the PC controlled DMD. A micro chamber integrated with an extremely low dead volume reagent injector for real-time monitoring of living cell was developed. In order to realize specific introduction of the reagent, we also developed the 3D sheath flow scanner. For high throughput analysis, a prototype of 8 diagnostic micro chambers having passive distributing facilitates simultaneous cell injection. On the other hand, microfluidic systems using sheath flow behavior in the microchannel for biological cell sorting were also developed. The flow system can sort two different cells to 16 outlet microchambers. To realize high efficient reaction of DNA and protein, two phase micro droplets of organic/water and three phase micro droplets of organic/water/air genelation fluidic devices were proposed.

  • Research Products

    (29 results)

All 2007 2006 2005 2004

All Journal Article (25 results) Book (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Three-Dimensional Sheath Flow Sorting Microsystem Using Thermosensitive Hydrogel2007

    • Author(s)
      T.Arakawa, Y.Shirasaki, T.Aoki, T.Funatsu, S.Shoji
    • Journal Title

      Sensors and Actuators A 135

      Pages: 99-105

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Three-Dimensional Sheath Flow Sorting Microsystem Using Thermosensitive Hydrogel2007

    • Author(s)
      T.Arakawa, Y.Shirasaki, T.Aoki, T.Funatsu, S.Shoji
    • Journal Title

      Sensors and Actuators A135

      Pages: 99-105

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow2006

    • Author(s)
      Y.Shirasaki, J.Tanaka, H.Makazu, K.Tashiro, S.Shoji, S.Tsukita, T.Funatsu
    • Journal Title

      Analytical Chemistry 78.3

      Pages: 695-701

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Improved Inclined Multi-Lithography Using Water As Exposure Medium And Its 3-D Mixing Microchannel Application2006

    • Author(s)
      H.Sato, D.Yagyu, S.Ito, S.Shoji
    • Journal Title

      Sensors and Actuators A 128

      Pages: 183-190

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Improved Passive Cell Distributing Method for Micro Cellular Diagnostic Well Array2006

    • Author(s)
      K.Kawai, M.Kanai, H.Nakanishi, S.Shoji
    • Journal Title

      Japanese Journal of Applied Physics 45.6B

      Pages: 5607-5613

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Real-Time Monitoring of Antibody Secretion from Hybridomas on a Microchip by Time-Resolved Luminescence Anisotropy Analysis2006

    • Author(s)
      T.Munaka, H Abe, M.Kanai, T.Sakamoto, H.Nakanishi, T.Yamaoka, S.Shoji, A.Murakami
    • Journal Title

      Analytical Biochemistry 353.1

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An All SU-8 Microfluidic Chip with Built-in 3D Fine Microstructures2006

    • Author(s)
      H.Sato, H.Matsumura, S.Keino, S.Shoji
    • Journal Title

      Journal of Micromechanics and Microengineering 16

      Pages: 2318-2322

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High-Speed Particles and Biomolecules Sorting Microsystem Using Thermosensitive Hydrogel2006

    • Author(s)
      T.Arakawa, Y.Shirasaki, T.Izumi, T.Aoki, H.Sugino, T.Funatsu, S.Shoji
    • Journal Title

      Measurement Science and Technology 17

      Pages: 3141-3146

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Spatially Focused Reagent Injection System Using 3-D Sheath Flow Scanner Integrated With SiO_2 Mesh Strainer2006

    • Author(s)
      Y.Suzuki, M.Kanai, T.Nishimoto, S.Shoji
    • Journal Title

      Tenth International Conference on Miniaturized Systems for Chemistry and Life Sciences : μTAS 2006

      Pages: 630-632

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Continuous Liquid and Particle Handlings in Micro Channel2006

    • Author(s)
      S.Shoji, H.Sato, T.Arakawa, S.Kanai
    • Journal Title

      Sixth Asia-Pacific International Symposium on Microscale Separations and Analysis : APCE 2006

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Improved Inclined Multi-Lithography Using Water As Exposure Medium And Its 3-D Mixing Microchannel Application2006

    • Author(s)
      H.Sato, D.Yagyu, S.Ito, S.Shoji
    • Journal Title

      Sensors and Actuators A, Vol.128

      Pages: 183-190

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Improved Passive Cell Distributing Method for Micro Cellular Diagnostic Well Array2006

    • Author(s)
      K.Kawai, M.Kanai, H.Nakanishi, S.Shoji
    • Journal Title

      Japanese Journal of Applied Physics, Vol.45, No.6B

      Pages: 5607-5613

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Real-Time Monitoring of Antibody Secretion from Hybridomas on a Microchip by Time-Resolved Luminescence Anisotropy Analysis2006

    • Author(s)
      T.Munaka, H Abe, M.Kanai, T.Sakamoto, H.Nakanishi, T.Yamaoka, S.Shoji, A.Murakami
    • Journal Title

      Analytical Biochemistry, Vol.353, No.1

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An All SU-8 Microfluidic Chip with Built-In 3D Fine Microstructures2006

    • Author(s)
      H.Sato, H.Matsumura, S.Keino, S.Shoji
    • Journal Title

      Journal of Micromechanics and Microengineering 16

      Pages: 2318-2322

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Picoliter Volume Glass Tube Array Fabricated by Si Electrochemical Etching Process2005

    • Author(s)
      H.Sato, T.Homma, K.Mori, T.Osaka, S.Shoji
    • Journal Title

      Electrochimica Acta 51

      Pages: 844-848

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] PDMS Microchannels with Slanted Grooves Embedded in Three Walls to Realize Efficient Spiral Flow2005

    • Author(s)
      H.Sato, S.Ito, K.Tajima, N.Orimoto, S.Shoji
    • Journal Title

      Sensors and Actuators A 119

      Pages: 365-371

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High Efficiency Electrochemical Immuno Sensors Using 3D Comb Electrodes2005

    • Author(s)
      N.Honda, M.Inaba, T.Katagiri, S.Shoji, H.Sato, T.Homma, T.Osaka, M.Saito, J.Mizuno, Y.Wada
    • Journal Title

      Biosensors and Bioelectronics 20.11

      Pages: 2306-2309

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Picoliter Volume Glass Tube Array Fabricated by Si Electrochemical Etching Process2005

    • Author(s)
      H.Sato, T.Homma, K.Mori, T.Osaka, S.Shoji
    • Journal Title

      Electrochimica Acta Vol.51

      Pages: 844-848

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] PDMS Microchannels with Slanted Grooves Embedded in Three Walls to Realize Efficient Spiral Flow2005

    • Author(s)
      H.Sato, S.Ito, K.Tajima, N.Orimoto, S.Shoji
    • Journal Title

      Sensors and Actuators A, Vol.119

      Pages: 365-371

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High Efficiency Electrochemical Immuno Sensors Using 3D Comb Electrodes2005

    • Author(s)
      N.Honda, M.Inaba, T.Katagiri, S.Shoji, H.Sato, T.Homma, T.Osaka, M.Saito, J.Mizuno, Y.Wada
    • Journal Title

      Biosensors and Bioelectronics, Vol.20, Issue 11

      Pages: 2306-2309

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow2005

    • Author(s)
      Y.Shirasaki, J.Tanaka, H.Makazu, K.Tashiro, S.Shoji, S.Tsukita, T.Funatsu
    • Journal Title

      Analytical Chemistry, Vol.78, No.3

      Pages: 695-70

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Micro Chamber for Cellar Analysis Integrated with Negligible Dead Volume Sample Injector2004

    • Author(s)
      M.Kanai, H.Abe, T.Munaka, Y.Fujiyama, D.Uchida, A.Yamayoshi, H.Nakanishi, A.Murakami, S.Shoji
    • Journal Title

      Sensors and Actuators A 114

      Pages: 129-134

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A Disposable, Dead Volume-Free and Leak-Free In-Plane PDMS Microvalve2004

    • Author(s)
      J.S.Go, S.Shoji
    • Journal Title

      Sensors & Actuators A 114

      Pages: 438-444

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Micro Chamber for Cellar Analysis Integrated with Negligible Dead Volume Sample Injector2004

    • Author(s)
      M.Kanai, H.Abe, T.Munaka, Y.Fujiyama, D.Uchida, A.Yamayoshi, H.Nakanishi, A.Murakami, S.Shoji
    • Journal Title

      Sensors and Actuators A, 114

      Pages: 129-134

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Disposable, Dead Volume-Free and Leak-Free In-Plane PDMS Microvalve2004

    • Author(s)
      J.S.Go, S.Shoji
    • Journal Title

      Sensors & Actuators A, 114

      Pages: 438-444

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] ポリマーフロンティア21シリーズ「ナノバイオー微細加工と計測技術の新展開」2005

    • Author(s)
      庄子習一
    • Total Pages
      19
    • Publisher
      高分子学会
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] フルオロカーボン微小構造体の製造方法、フルオロカーボン微小構造体およびマイクロシステム2007

    • Inventor(s)
      庄子習一, 荒川貴博, 草川寛之
    • Industrial Property Rights Holder
      早稲田大学
    • Industrial Property Number
      特願2007-010065号
    • Filing Date
      2007-01-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] マイクロカプセル、マイクロカプセル製造装置及びマイクロカプセル製造方法2006

    • Inventor(s)
      庄子習一, 荒川貴博, 山本嵩洋
    • Industrial Property Rights Holder
      早稲田大学
    • Industrial Property Number
      特願2006-253682号
    • Filing Date
      2006-09-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 微粒子分別マイクロシステムおよび微粒子分別方法2005

    • Inventor(s)
      荒川貴博, 白崎善隆, 船津高志, 庄子習一
    • Industrial Property Rights Holder
      早稲田大学
    • Industrial Property Number
      特願2005-0345393
    • Filing Date
      2005-11-30
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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