2007 Fiscal Year Final Research Report Summary
Preservation and utilization of genetic resources of domestic ruminants, based on studies of the origin and phylogeny
| Project/Area Number |
16201046
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Resource conservation science
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| Research Institution | Nagoya University |
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Principal Investigator |
NAMIKAWA Takao Nagoya University, Graduate School of Bioagricultural Sciences, Professor (70111838)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Takashi Tokyo University of Agriculture, Faculty of Agriculture, Professor (90078147)
MAEDA Yoshizane Kagoshima University, Faculty of Agriculture, Professor (50041661)
MANNEN Hideyuki Kobe University, Graduate School of Science and Technology, Associate Professor (20263395)
TANAKA Kazuaki Azabu University, Scbool of Veterinary Medicine, Lecturer (50345873)
YAMAGATA Takahiro Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor (50242847)
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| Project Period (FY) |
2004 – 2007
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| Keywords | ruminants / bovine / water buffalo / functional gene / punon gene / calpine gene / native animals / genetic resource |
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| Research Abstract |
We have collected several thousands of DNA samples of ruminants, such as cattle, water buffaloes, sheep, goats, pigs, etc, in Japan and Asian countries through overseas scientific surveys and present research project Those invaluable DNA samples were data-filed by the present investigators in order to facilitate them to be supplied far researchers elsewhere. On the other hand, we analyzed those samples to get knowledge of the genetic-variability and -differentiation between local populations, and genotypic variations of several functional genes.
1.Research on genetic differentiation between populations
(1) Domestic pigs and wild boars from respective areas of South and Southeast Asian continent were analyzed of the r mtDNA haplotypes, The result showed that some pigs carried identical mtDNA haplotypes to those of wild boars inhabiting same area, i.e. polyphyletic origin of the pig.
2.Researches on genotypic variations of ruminant functional genes
(1) Expression of a cluster of genes related to regulates of seasonal breeding of animals was examined by methods of system biology, we found that the TSH. elevation in MBH increased DIO2 gene expression followed by other gene expressions, indicating TSH in MBH is a trigger for the photoperiodic activities.
(2) Polymorphism in B2C high-sulfur wool protein alleles was examined in sheep from China and East Asia and compared to European breeds. The local breeds of northern part of the area showed a similar allelic compositions to European breeds, but the southern local populations were marked different from them in the allelic frequencies.
(3)Purion protein genotypes were examined in a variety of local or native populations, and found the scrapie sensitive genotype ARQ/ARQ is highly prevalent in East Asian local breeds or native populations.
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[Journal Article] Common erythrocyte antigens among cattle and their close relatives revealed by analysis of monoclonal antibodies2008
Author(s)
Nomura, K., K. Kurogi, M. Morita, M. Kanemaki, E. Saitoh, S. Kawakami, C. Cho, Sutopo, M.O. Faruque, T. Amano
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Journal Title
Journal of Agricultural Sciences. TUA 53(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mitochondrial diversity of native pigs in the mainland South and Southeast Asian countries and its relationships between local and wild boars2008
Author(s)
Tanaka, K., Y. Iwaki, T. Takizawa, T. Dorji, G. Tsering, Y. Kuroswa, Y. Maeda, H. Mannen, K. Nomura, V.-B. Dang, L. Chuum-Phith, B. Bouahom, Y. Yamamoto, T. Daing, T. Namikawa
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Journal Title
Animal Science Journal 79(4)(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Thyrotrophin in the pars tuberalis triggers photoperiodic response2008
Author(s)
Nakao, N., H. Ono, T. Yamamura, T. Anraku, T. Takagi, K. Higashi, S. Yasuo, Y. Katou, S. Kageyama, Y. Uno, T. Kasukawa, M. Iigo, P.J. Sharp, A. Iwasawa, Y. Suzuki, S. Sugano, T. Niimi, M. Mizutani T. Namikawa, S. Ebihara, H.R. Ueda, T. Yoshimura
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Journal Title
Nature 452
Pages: 317-322
Description
「研究成果報告書概要(欧文)」より
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