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2006 Fiscal Year Final Research Report Summary

Evaluation of Genetic Diversity in and Molecular Genotyping of Asian Wheat Genetic Resources

Research Project

Project/Area Number 16201047
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Resource conservation science
Research InstitutionYokohama City University

Principal Investigator

SASANUMA Tsuneo  Yokohama City University, Kihara Institute for Biological Research, Assistant Professor, 木原生物学研究所, 助手 (70347350)

Co-Investigator(Kenkyū-buntansha) MIURA Hideho  Obihiro University of Agriculture and Veterinary Medicine, School of Veterinary and Medicine, Professor, 畜産学部, 教授 (10173981)
KAWAHARA Taihachi  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (20115827)
MORI Naoki  Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60230075)
KATO Kenji  Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (40161096)
Project Period (FY) 2004 – 2006
KeywordsWheat / Aegilops / Landraces / Genetic diversity / Genotyping / Heading trait / Seed domancy
Research Abstract

Evaluation of genetic diversity in and molecular genotyping of genetic resources of wheat and its relatives were conducted. Modernization of agriculture has produced many modern wheat cultivars adapted with irrigation, chemical nutrition and mechanization, and today supported a huge world food demand. The modernization, however, resulted in loss of genetic diversity, thus we face the limitation of the increased yield and fragility against coming world environment changes. To overcome the problems, the genetic diversity present in wild species and are reconsidered, and the importance of evaluation and utilization of the potential genetic diversity are noticed. The establishment of the evaluation method of such diversity and the elucidation of the evolutionary process which has produced the diversity are also considered to be valuable.
The spread of cultivated wheat from its original place to the west and east produced a lot of differentiated local varieties, namely landraces, adapted wit … More h various local climates. Japanese wheat research community have collected wild species and landraces and have long-time conserved them as genetic resources. This research project is a collaborating research formed by researchers who have conserved the genetic resources and investigated genetic diversity or evolutionary processes. In this project approximately 600 cultivated and 700 wild accessions were investigated and the following outcomes were obtained.
1. The basic information of morphology, heading trait, salt tolerance and seed dormancy for each accession were evaluated and recorded. The level of diversity was considerably high in both wild and cultivated species.
2. Core collections (representative accessions covering whole population) were selected based on DNA marker and AFLP, and methods for conservation were proposed.
3. Multiple origins in both domestication and polyploidization processes were revealed, which are clearly contrasted with previous theories.
4. The genes controlling or related with heading trait and seed dormancy were identified using DNA marker. Genetic variation among accessions in those genes was revealed. These results enable us to use DNA marker to distinguish variety or to reveal evolutionary process.
5. The accessions used in this study and data obtained were registered in National BioResources Project (NBRP) database and free access and easy utilization were enabled. Less

  • Research Products

    (27 results)

All 2007 2006 2005 2004 Other

All Journal Article (27 results)

  • [Journal Article] Allelic variation of heading trait-related genes, essential for wide adaptability of wheat, and its application to wheat breeding.2007

    • Author(s)
      Kato K.
    • Journal Title

      Gamma Field Symposia (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy.2007

    • Author(s)
      Nakamura S., Komatsuda T., Miura H.
    • Journal Title

      Theor. Appl. Genet. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect.2007

    • Author(s)
      Mori M., Uchino N., Chono M., Kato K., Miura H.
    • Journal Title

      Theor.Appl.Genet. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Aegilops tauschii : genetic variation in Iran.2006

    • Author(s)
      Dudnikov A.J., Kawahara T.
    • Journal Title

      Genet. Resour. Crop Evol. 53

      Pages: 579-586

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic diversity and phylogenetic relationships among east Asian common wheat (Triticum aestivum L.) populations, revealed by the analysis of five isozymes.2006

    • Author(s)
      Ghimire S.K., Akashi Y., Masuda A., Washio T., Nishida H., Zhou Y.H., Yen C., Qi X., Li Z., Yoshino H., Kato K.
    • Journal Title

      Breed. Sci. 56

      Pages: 379-387

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ishii T., Arimura S., Ikeda N., Kamijima O., Mori N.
    • Journal Title

      Genes Genet. Syst. 81

      Pages: 211-214

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs.2006

    • Author(s)
      Kottearachchi N.S., Uchino N., Kato K., Miura H.
    • Journal Title

      Euphytica 152

      Pages: 421-428

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 穂発芽耐性と種子休眠性の遺伝.2006

    • Author(s)
      三浦秀穂
    • Journal Title

      穂発芽-国産小麦の品質向上をめざして(穂発芽研究会編)

      Pages: 110-128

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2006

    • Author(s)
      Onishi I., Hongo A., Sasakuma T., Kawahara T., Kato K., Miura H.
    • Journal Title

      Genet. Resour. Crop Evol. 53

      Pages: 985-992

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. biuncialis and Ae. ovata.2006

    • Author(s)
      Sasanuma T., Kadosumi S., Kawahara T.
    • Journal Title

      Wheat Inf. Serv. 101

      Pages: 11-12

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pattern and rate of indel evolution inferred from whole chloroplast intergenic regions in sugarcane, maize and rice.2006

    • Author(s)
      Yamane K., Yano K., Kawahara T.
    • Journal Title

      DNA Research 13

      Pages: 197-204

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Aegilops tauschii : genetic variation in Iran.2006

    • Author(s)
      Dudnikov A.J., Kawahara T.
    • Journal Title

      Resour.Crop Evol. 53

      Pages: 579-586

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ghimire S.K., Akashi Y., Masuda A., Washio T., Nishida H., Zhou Y.H., Yen C., Qi X., Li Z., Yoshino H., Kato K.
    • Journal Title

      Breed.Sci. 56

      Pages: 379-387

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mitochondrial microsatellite variability in common wheat and its ancestral species.2006

    • Author(s)
      Ishii T., Arimura S., Ikeda N., Kamijima O., Mori N.
    • Journal Title

      Genes Genet.Syst. 81

      Pages: 211-214

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetics in tolerance to pre-harvest sprouting and seed dormancy. (in Japanese)2006

    • Author(s)
      Miura H.
    • Journal Title

      Pre-Harvest Dormancy-head for improvement of quality of domestic wheat

      Pages: 110-128

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Variation and segregation for rachis fragility in spelt wheat, Triticum spelta L.2006

    • Author(s)
      Onishi I., Hongo A., Sasakuma T., Kawahara T., Kato K., Miura H.
    • Journal Title

      Genet.Resour.Crop Evol. 53

      Pages: 985-992

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. bluncialis and Ae. ovata.2006

    • Author(s)
      Sasanuma T., Kadosumi S., Kawahara T.
    • Journal Title

      Wheat Inf.Serv. 101

      Pages: 11-12

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic diversity and geographical differentiation in Asian common wheat (Triticum aestivum L.), revealed by the analysis of peroxidase and esterase isozymes.2005

    • Author(s)
      Ghimire S.K., Akashi Y., Maitani C., Nakanishi M., Kato K.
    • Journal Title

      Breed. Sci. 55

      Pages: 175-185

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. colomnaris and Ae. triaristata revealed based on the polyporphism of a genome-specific PCR fragment.2005

    • Author(s)
      Kadosumi S., Kawahara T., Sasanuma T.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 105-111

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 穂発芽耐性小麦の開発.2005

    • Author(s)
      三浦秀穂
    • Journal Title

      農林水産技術 研究ジャーナル 28 (7)

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mapping QTLs for grain dormancy on wheat 3A and group 4 chromosomes, and their combined effect.2005

    • Author(s)
      Mori M., Uchino N., Chono M., Kato K., Miura H.
    • Journal Title

      Theor. Appl. Genet. 110

      Pages: 1315-1323

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intra and interspecific phylogenetic relationships among diploid Triticum-Aegilops species based on base pair substitutions, indels and microsatellites in chloroplast non-coding sequences.2005

    • Author(s)
      Yamane K., Kawahara T.
    • Journal Title

      American J. Bot. 92

      Pages: 1887-1898

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic diversity and geographical differentiation in Asian common wheat (Triticum aestivum L.), revealed by the analysis of peroxidase and esterase isozymes.2005

    • Author(s)
      Ghimire S.K., Akashi Y., Maitani C., Nakanishi M., Kato K.
    • Journal Title

      Breed.Sci. 55

      Pages: 175-185

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Multiple origins of U genome in two UM genome tetraploid Aegilops species, Ae. colomnaris and Ae. trIaristata revealed based on the polyporphism of a genome-specific PCR fragment.2005

    • Author(s)
      Kadosumi S., Kawahara T., Sasanuma T.
    • Journal Title

      Genes Genet.Syst. 80

      Pages: 105-111

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of wheat with tolerance to pre-harvest sprouting. (in Japanese)2005

    • Author(s)
      Miura H.
    • Journal Title

      Research Journal of Food and Agriculture 28

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Allelic variation at the VRN-1 promoter region in polyploid wheat.2004

    • Author(s)
      Yan L., Helguera M., Kato K., Fukuyama S., Sherman J., Dubcovsky J.
    • Journal Title

      Theor. Appl. Genet. 109

      Pages: 1677-1686

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Allelic variation at the VRN-1 promoter region in polyploid wheat.

    • Author(s)
      Yan L., Helguera M., Kato K., Fukuyama S., Sherman J.
    • Journal Title

      Theor.Appl.Genet.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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