2006 Fiscal Year Final Research Report Summary
Interaction between Metallome and Proteome in the Marine Ecosystem
| Project/Area Number |
16204046
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Geochemistry/Astrochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
SOHRIN Yoshiki Kyoto University, Institute for Chemical Research, Professor, 化学研究所, 教授 (50197000)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Kei Kochi University, Center for Advanced Marine Core Research, Associate Professor, 海洋コア総合研究センター, 助教授 (70324697)
NORISUYE Kazuhiro Kyoto University, Institute for Chemical Research, Assistant Professor, 化学研究所, 助手 (50335220)
SUZUKI Koji Hokkaido University, Faculty of Environmental Earth Science, Associate Professor, 大学院地球環境科学研究科, 助教授 (40283452)
HASEGAWA Hiroshi Kanazawa University, Graduate School of Natural Science and Technology, Associate Professor, 大学院自然科学研究科, 助教授 (90253335)
SOHRIN Rumi Shizuoka University, Faculty of Science, Assistant Professor, 理学部, 助手 (00343195)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Oceanography / Analytical chemistry / Biogeochemistry / Trace metals / Phytoplankton / Bacteria / Pacific Ocean / Sulu Sea |
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| Research Abstract |
1.Development of chemical speciation and in situ determination of bioactive trace metals
(1)Determination of suspended particulate Co, Ni, Cu, Zn, Cd and Pb has been developed.
(2)Determination of dissolved and acid-dissolvable Zr, Hf, Nb, Ta and W has been developed.
(3)In-situ flow analysis of alkalinity and total carbonate has been developed.
2.Development of analytical methods for enzymes and proteins of phytoplankton and bacteria
(1)Detection of Fe-S protein PsaC and PsbA(D1) in photosynthetic system has been developed.
(2)Anti-ferredoxin and anti-flavodoxin antibodies, which cross-react with ferredoxin and flavodoxin of marine diatoms, respectively, have been developed using synthetic peptides in order to estimate their Fe stress.
3.Observation of the variation and relationship of metallome and proteome
(1)Different distributions of Fe, Co, Ni, Cu, Zn and Cd in seawater between the Sulu Sea, South China Sea and Pacific Ocean were observed.
(2)During the second mesoscale iron enrichment experiments in the western subarctic North Pacific (SEEDS-II), the effects of iron enrichment on the phytoplankton community structure and the speciation of bioactive trace metals were examined.
(3)On the basis of analysis of photosynthetic proteins, Fe in seawater has been proved to be a major factor controlling the spring bloom of diatoms in the Oyashio region.
(4)Diurnal dynamics of biomass of nano-flagellates and ciliates in Suruga Bay was investigated.
(5)The effects of trace metals on the activity of extracellular enzymes of microbes have been proved.
4.Study on metal uptake mechanism of phytoplankton in culture
(1) The size distribution of Fe in culture media of phytoplankton was investigated. Fe ligands exuded from phytoplankton were analyzed by LC-MS.
(2)Membrane proteins revealed in red tide forming phytoplankton Prymnesium parvum under Fe deficiency were analyzed.
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