2007 Fiscal Year Final Research Report Summary
Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method
| Project/Area Number |
16206076
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
NAKANISHI Kazuhiro Okayama University, Graduate School of Natural Science and Technology, Professor (90026584)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Koreyoshi Okayama University, Graduate School of Natural Science and Technology, Associate Professor (70294436)
IMANAKA Hiroyuki Okayama University, Graduate School of Natural Science and Tochnology, Assistant Professor (10379711)
|
|---|
| Project Period (FY) |
2004 – 2007
|
| Keywords | adsorption / adsorption equilibrium / protein / Peptide / metal oxide surface / plastic surface / cleaning / hydrogen peroxide-electrolysis cleaning |
|---|
| Research Abstract |
In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.
|
-
-
-
-
-
-
-
[Journal Article] Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate2007
Author(s)
Yoichi, Kumada, Chunhui, Zhao, Ryota, Ishimura, Hiroyuki, Imanaka, Korevoshi, Imamura, Kazuhiro, Nakanishi
-
Journal Title
J. Biotechnol 128(2)
Pages: 354-361
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Journal Article] Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins2006
Author(s)
Kumada Y., Y., Tokunaga, H., Imanaka, K., Imamura, T., Sakiyama, S., Katoh, K., Nakanishi
-
Journal Title
Biotechnol Progress 22(2)
Pages: 401-405
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Decomposition and removal of organic substances adsorbed on the metal oxide surface using H202 treatment2007
Author(s)
K., Imamura, T., Kuroda, M., Kozaki, H., Imanaka, K., Nakanishi
Organizer
The 72th Autumn Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Hokkaido Univ
Year and Date
2007-09-13
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] Detection of protein-protein interaction using PS affinity peptides2007
Author(s)
Y., Kumada, C., Zhao, H., Imanaka, K., Imamura, K., Nakanishi
Organizer
The 72th Annual Branch Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Tokushima Univ
Year and Date
2007-03-20
Description
「研究成果報告書概要(欧文)」より
-
-
[Presentation] Controll of adsorption characteristics of proteins linked with the metal nxide affinity pentide2006
Author(s)
K., Yanagita, M., Nakaizumi, D., Yamazumi, H., Imanaka, K., Imamura, K., Nakanishi
Organizer
The Tokushima Branch Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Tokushima Univ
Year and Date
2006-10-28
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
[Presentation] Protein-protein interaction analysis using an affinity peptide2006
Author(s)
Y., Kumada, R., Ishimura, H., Uesaki, H., Imanaka, K., Imamura, K., Nakanishi
Organizer
The 38h Autumnl Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Fukuoka Univ
Year and Date
2006-09-17
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Controll of protein orientation on the metal oxide surfaces2006
Author(s)
K., Yanagata, M., Nakazumi, H., Imanaka, K., Imamura, K., Nakanishi
Organizer
The 38h Autumnl Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Fukuoka Univ
Year and Date
2006-09-17
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] Screening of peptides that have a apecifi affinity to the metal oxide surfaces and adsorption characteristics of enzymes2006
Author(s)
H., Imanaka, Y., Satake, M., Nakazumi, K., Imamura, K., Nakanishi
Organizer
The 71th Annual Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Tokyo Inst. Technol
Year and Date
2006-03-29
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Rapid immunoassay using proteins linked with polystyrene specific affinity peptides2006
Author(s)
Y., Kumada, H., Imanaka, K., Imamura, S., Katoh, K., Nakanishi
Organizer
The 71th Anual Meeting of the Society of Chemical Engineers, Japan (2006.3.29) Tokyo Inst. Technol
Place of Presentation
K., Kanamoto, H., Imanaka, K., Imamura, K., Nakanishi
Year and Date
2006-03-29
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Preparation of proteins linked with stainless steel surface specific peptides and their adsorption characteristics2006
Author(s)
D., Imaishi, Y., Kumada, H., Imanaka, K., Imamura, T., Sakiyama, K., Nakanishi
Organizer
The 14th Chugoku-Shikoku Branch Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Fukuyama Univ
Year and Date
2006-01-28
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Presentation] Enzymes linked with oligopeptides having a specific affinity to nolystvrene surfaces2005
Author(s)
D., Imaishi, H., Imanaka, K., Imamura, T., Sakiyama, K., Nakanishi
Organizer
The 37th Autumn Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Okayama Univ
Year and Date
2005-09-15
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Adsorption characteristics of proteins linked with polystyrene specific pentides and its application2005
Author(s)
H., Tokunaga, Y., Kumada, D., Imaishi, H., Imanaka, T., Sakiyama, K., Imamura, K., Nakanishi
Organizer
The 37th Autumn Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Okayama Univ
Year and Date
2005-09-15
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Presentation] Fouling characteristics of soils composed of tannic acid-proteins and their cleaning characteristics by H202 electrolysis treatment2005
Author(s)
K., Imamura, Y., Aramoto, H., Imanaka, T., Sakiyama, T., Takahashi, K., Nakanishi
Organizer
The 6th (2005) Annual Meeting of the Japan Society for Food Engineers
Place of Presentation
Ohtsu
Year and Date
2005-07-29
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
[Presentation] Desorption characteristics of nroteins adsorbed on various metal oxide surfaces2005
Author(s)
K., Imamura, M., Oshita, Y., Aramoto, H., Imanaka, T., Sakiyama, K., Nakanishi
Organizer
The 2005th Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Hokaido Univ
Year and Date
2005-03-29
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Desorption characteristics of various proteins adsorbed on the metal oxide surfaces by H202 treatment and desorption accelerateine factors2005
Author(s)
K., Imamura, M., Oshita, M., Iwai, K., Kanamoto, H., Imanaka, K., Nakanishi
Organizer
The 69th Annual Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Nagoya Univ
Year and Date
2005-03-22
Description
「研究成果報告書概要(欧文)」より
-
-
[Presentation] Immobilization of enzymes on poyvstvrene surfaces using affinity peptides2005
Author(s)
H., Tokunaga, D., Imaishi, H., Imanaka, T., Sakiyama, K., Imakura, K., Nakanishi
Organizer
The 11th Chugoku-Shikoku Branch Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Okayama Univ
Year and Date
2005-01-22
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Presentation] Adsorption characteristics of various kinds of proteins on the metal oxide surfaces2004
Author(s)
M., Shimomura, S., Nagai, M., Oshita, K., Imamura, T., Sakiyama, K., Nakanishi
Organizer
The Yamaguchi Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Kaikyo Messe Shimonoseki
Year and Date
2004-09-27
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Adsorption characteristics of enzymes on the metal oxide surfaces and activity retaining2004
Author(s)
K., Yamagoe, T., Nagayasu, K., Matsumoto, K., Imamura, T., Sakiyama, K., Nakanishi
Organizer
The Yamaguchi Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Kaikyo Messe Shimonoseki
Year and Date
2004-09-27
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Effects of various factors on the cleaning characteristics in H202 electrolysis treatment2004
Author(s)
K., Imamura, I., Watanabe, M., Oshita, M., Iwai, T., Sakiyama, K., Nakanishi
Organizer
The Yamaguchi Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Kaikyo Messe Shimonoseki
Year and Date
2004-09-27
Description
「研究成果報告書概要(欧文)」より
-
-
[Presentation] Desorption characteristics of proteins adsorbed on the metal oxide surfaces by H202 electrolysis treatment2004
Author(s)
K., Imamura, I., Watanabe, M., Shinohara, Y., Aramoto, T., Sakiyama, K., Nakanishi
Organizer
The 5th (2004) Annual Meeting of the Japan Society for Food Engineers
Place of Presentation
Tokyo Univ
Year and Date
2004-08-03
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Presentation] Adsorption characteristics and activities of some enzymes on the metal oxide surfaces2004
Author(s)
K., Yamagoe, T., Nagavasu, K., Matsumoto, K. Imamura, T., Sakiyama, K., Nakanishi
Organizer
The 69th Annual Meeting of the Society of Chemical Engineers, Japan
Place of Presentation
Osaka Prefectural Univ
Year and Date
2004-04-04
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
