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2005 Fiscal Year Final Research Report Summary

Development of a hybrid artificial liver with hepatocytes derived embryonic stem cell for chronic liver failure patients

Research Project

Project/Area Number 16206079
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KAJIWARA Toshihisa  Kyushu University, Faculty of Engineering, Professor, 大学院・工学研究院, 教授 (10194747)

Co-Investigator(Kenkyū-buntansha) MIZUMOTO Hiroshi  Kyushu University, Faculty of Engineering, Associate Professor, 大学院・工学研究院, 助教授 (90346817)
NAKAZAWA Kohji  The University of Kitakyushu, Faculty of Environmental Engineering, Associate Professor, 国際環境工学部, 助教授 (00304733)
Project Period (FY) 2004 – 2005
Keywordsembryonic stem cell / organoid / differentiation / hepatocyte / hollow fiber / hybrid artificial liver / regenerative medicine
Research Abstract

We investigated the use of organoid culture technique to mediate hepatocyte differentiation of murine and primate embryonic stem cell. We used our original organoid culture technique, which induce cultured cells to form an organoid in lumen of hollow fibers by centrifugal force.
Mouse ES cells formed an organoid inside of hollow fibers. The ES cell proliferated for more than 20 days of culture and high density culture was achieved. The expressions of mRNAs of hepatocyte specific genes were detected at 15 days of culture, and were sustained for more than 30 days of culture. The functions of albumin secretion and ammonia removal were evaluated under hepatocyte differentiation culture condition. The level of these functions of the ES cells per unit volume of cell culture space increased as culture time, and reached the same level of primary mouse hepatocytes.
Cynomolgus monkey ES cell was also investigated as a model of primate ES cell. Cynomolgus monkey ES cells formed an organoid and expressed some liver-specific functions such as albumin secretion and ammonia removal. The activity of ammonia removal of ES cells organoid per unit volume of cell culture space was roughly comparable to that of primary hepatocytes and human hepatoblastoma cell lines.
In conclusion, our developed novel organoid culture technique directed both mouse and cynomolgus monkey ES cells along a hepatocyte lineage. This culture technique seems to be a promising technique to develop a hybrid artificial liver.

  • Research Products

    (25 results)

All 2006 2005 2004 Other

All Journal Article (19 results) Book (6 results)

  • [Journal Article] Differentiation effects by the combination of spheroid formation and sodium butyrate treatment in human hepatoblastoma cell line (Hep G2) : a possible cell source for hybrid artificial liver2006

    • Author(s)
      J.Fukuda et al.
    • Journal Title

      Cell Transplantation Vol.14, No.10

      Pages: 819-827

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 最新医療を支える成型加工技術の現状2005

    • Author(s)
      中澤浩二 他
    • Journal Title

      プラスチックス 56(9)

      Pages: 147-152

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 人工臓器開発における化学工学の役割2005

    • Author(s)
      水本博 他
    • Journal Title

      ケミカルエンジニヤリング 50(9)

      Pages: 688-693

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] オルガノイド培養法を利用したハイブリッド型人工肝臓の開発2005

    • Author(s)
      青木健太朗 他
    • Journal Title

      化学工学シンポジウムシリーズ 79

      Pages: 80-83

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differentiation effects by the combination of spheroid formation and sodiumbutyrate treatment in human hepatoblastoma cell line (Hep G2) : a possible cell source for hybrid artificial liver2005

    • Author(s)
      J.Fukuda et al.
    • Journal Title

      Cell Transplantation 14(10)

      Pages: 819-827

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hepatocyte organoid culture in elliptic hollow fibers to develop a hybrid artificial liver2005

    • Author(s)
      J.Fukuda et al.
    • Journal Title

      The International Journal of Artificial Organs Vol.27, No.12

      Pages: 1091-1099

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The current situation of polymer processing technique to support the latest medical care2005

    • Author(s)
      K.Nakazawa et al.
    • Journal Title

      Japan Plastics Vol.56, No.9

      Pages: 1047-1052

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Role of chemical engineering on artificial organs development2005

    • Author(s)
      H.Mizumoto et al.
    • Journal Title

      Chemical Engineering Vol.50, No.9

      Pages: 688-693

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of a hybrid artificial liver using hepatocyte organoid culture2005

    • Author(s)
      K.Aoki et al.
    • Journal Title

      Chemical Engineering Symposium Series 79

      Pages: 80-83

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 肝細胞オルガノイド培養法を利用したハイブリッド型人工肝臓2004

    • Author(s)
      中澤浩二 他
    • Journal Title

      医工学治療 16(3)

      Pages: 163-167

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hybrid artificial liver with polyurethane foam / spheroid culture for treatment of acute liver failure2004

    • Author(s)
      T.Kajiwara et al.
    • Journal Title

      Proceedings of The 10th Asian Pacific Confederation of Chemical Engineering Congress

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of a hybrid artificial liver module having liver lobule-like strucuture2004

    • Author(s)
      H.Mizumoto et al.
    • Journal Title

      Proceedings of The 10th Asian Pacific Confederation of Chemical Engineering Congress

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Organoid-sheet formation for hepatocyte and myocardial cell culture2004

    • Author(s)
      K.Ishihara et al.
    • Journal Title

      Proceedings of The 10th Asian Pacific Confederation of Chemical Engineering Congress

      Pages: 1-9

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] hepatocyte organoid culture in elliptic hollow fibers to develop a hybrid artificial liver2004

    • Author(s)
      J.Fukuda et al.
    • Journal Title

      The International Journal of Artificial Organs 27(12)

      Pages: 1091-1099

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A hybrid artificial liver using hepatocyte organoid2004

    • Author(s)
      K.Nakazawa et al.
    • Journal Title

      Therapeutics & Engineering Vol.16, No.3

      Pages: 163-167

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hybrid artificial liver with polyurethane foam/spheroid culture for treatment of acute liver failure2004

    • Author(s)
      T.Kajiwara et al.
    • Journal Title

      Proceedings of The 10th Asian Pacific Confederation of Chemical Engineering Congress

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of a hybrid artificial liver module having liver lobule-like structure2004

    • Author(s)
      H.Mizumoto et al.
    • Journal Title

      Proceedings of The 10th Asian Pacific Confederation of Chemical Engineering Congress

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Formation of a sheet-shaped organoid using rat hepatocytes for long-term maintenance of liver-specific functions

    • Author(s)
      K.Ishihara et al.
    • Journal Title

      The International Journal of Artificial Organs (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Formation of a sheet-shaped organoid using rat hepatocytes for long-term maintenance of liver-specific functions

    • Author(s)
      K.Ishihara et al.
    • Journal Title

      The International Journal of Artificial Organs (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 生体工学概論(6.2 人工臓器工学)2006

    • Author(s)
      水本博 他
    • Total Pages
      237
    • Publisher
      コロナ社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Artificial Organs, Bioengineering and Biomedical Engineering, Chap.6, Sec.22005

    • Author(s)
      H.Mizumoto et al.
    • Total Pages
      201-210
    • Publisher
      Corona Publishing Co.Ltd.
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 化学工学ハンドブック(9.4細胞培養工学,9.4.1)2004

    • Author(s)
      水本博 他
    • Total Pages
      591
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 繊維便覧(第4部門4・11・1節 人工肝臓)2004

    • Author(s)
      福田淳二 他
    • Total Pages
      1009
    • Publisher
      丸善株式会社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Animal cell culture, Handbook of Chemical Engineering, Chap.9, Sec.9.4.12004

    • Author(s)
      H.Mizumoto et al.
    • Total Pages
      480-486
    • Publisher
      Asakura Co.Ltd.
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Artificial liver, Handbook of Textile, Part.4, Sec.4.11.12004

    • Author(s)
      J.Fukuda et al.
    • Total Pages
      831
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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