2006 Fiscal Year Final Research Report Summary
Molecular mechanism of sex determination in lepidopteran insects-The sex chromosomes regulate sex-specific splicing of dsx homologs.
| Project/Area Number |
16208006
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHIMADA Toru The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院農学生命科学研究科, 教授 (20202111)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Hiroaki Tokyo University of Agriculture and Technology, Department of Biological Production Faculty of Agriculture, Assistant Professor, 大学院共生科学技術研究部, 助手 (80222660)
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| Project Period (FY) |
2004 – 2006
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| Keywords | W chromosome / gene dosage compensation / sex-specific splicing / sex-determining genes / DM domain / RNAi / non-coding RNA / oogenesis |
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| Research Abstract |
1.Molecular mechanism of sex-specific alternative splicing in Bmdsx.
Bmdsx is a sex determining gene located on the 25^<th> chromosome in the silkworm, Bombyx mori. A mini Bmdsx gene containing exons 1-5 and mini intorons 2-4, which contained only the 5' and 3' ends sequences, was constructed and introduced into the germ line of the silkworm by the piggyBac-based transformation system. The mini Bmdsx transgene was successfully transcribed and spliced into the sex-dependent mRNAs in the transgenic silkworms. We also found that the 5' end sequences contained a cis element required for the male-specific splicing. In the UV-cross link experiment, a protein, p40, was bound to the cis element. We performed an RNAi experiment and proved that the p40 gene was essential for the male-type splicing of Bmdsx.
2.Female determining gene on the W chromosome.
The W chromosome should encode a potent female determinant for the silkworm though it is unknown. We mapped the female determinant into the Rikishi region using various kind of the chromosome aberrations. Although a few protein-coding genes were found in the Rikishi region, they are not found on the W chromosome of a closely-related species, Bombyx mandarina.
3.Structure and function of the Z chromosome.
The genome information predicted 655 protein-coding genes on the Z chromosome. The genes for nervous and muscular systems in the males tend to locate on the Z chromosome. For example, three of the four circadian clock components (per, tim, clk, cyc) were mapped onto the Z chromosome.
4.Sexual differentiation of germ cells, especially differentiation of the oocyte.
A SAGE analysis were performed to analyze stage-dependent gene expression in oocyte development. We noticed that a novel non-coding RNA (Ovachamp) was abundantly expressed in the ovary. The RNAi of Ovachamp showed an interesting phenotype, i. e. undeveloped oocytes, suggesting that this gene is essential for oogenesis.
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[Journal Article] Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori.2007
Author(s)
Tsuguru Fujii, Takeshi Yokoyama, Osamu Ninagi, Kowa Kakehashi, Yoshiaki Obara, Mitsuru Nenoi, Takahiro Ishikawa, Kazuei Mita, Toru Shimada, Hiroaki Abe
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori2007
Author(s)
Tsuguru Fujii, Takeshi Yokoyama, Osamu Ninagi, Kowa Kakehashi, Yoshiaki Ohara, Mitsuru Nenoi, Takahiro Ishikawa, Kazuei Mita, Toru Shimada, Hiroaki Abe
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes.2006
Author(s)
Tsuguru Fujii, Nobuhiko Tanaka, Takeshi Yokoyama, Osamu Ninaki, Toshikazu Oshiki, Akio Ohnuma, Yataro Tazima, Yutaka Banno, Masahiro Ajimura, Kazuei Mita, Motoaki Seki, Fumi Ohbayashi, Toru Shimada, Hiroaki Abe
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Journal Title
Genetica 127(1-3)
Pages: 253-265
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes2006
Author(s)
Tsuguru Fujii, Nobuhiko Tanaka, Takeshi Yokoyama, Osamu Ninaki, Toshikazu Oshiki, Akio Ohnuma, Yataro Tazima, Yutaka Banno, Masahiro Ajimura, Kazuei Mita, Motoaki Seki, Fumi Ohbayashi, Toru Shimada, Hiroaki Abe
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Journal Title
Genetica 127(1-3)
Pages: 253-265
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori.2005
Author(s)
Hiroaki Abe, Motoaki Seki, Fumi Ohbayashi, Nobuhiko Tanaka, Jun-ichi Yamashita, Tsuguru Fujii, Takeshi Yokoyama, Michiyoshi Takahashi, Yutaka Banno, Ken Sahara, Atsuo Yoshido, Jun-ichiro Ihara, Yuji Yasukochi, Kazuei Mita, Masahiro Ajimura, Masataka G. Suzuki, Toshikazu Oshiki, Toru Shimada
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Journal Title
Insect Molecular Biology 14(4)
Pages: 339-352
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Partial deletions of the W chromosome due to reciprocal transiocation in the silkworm Bombyx mori2005
Author(s)
Hiroaki Abe, Motoaki Seki, Fumi Ohbayashi, Nobuhiko Tanaka, Jun-ichi Yamashita, Tsuguru Fujii, Takeshi Yokoyama, Michiyoshi Takahashi, Yutaka Banno, Ken Sahara, Atsuo Yoshido, Jun-ichiro Ihara, Yuji Yasukochi, Kazuei Mita, Masahiro Ajimura, Masataka G. Suzuki, Toshikazu Oshiki, Toru Shimada
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Journal Title
Insect Molecular Biology 14(4)
Pages: 339-352
Description
「研究成果報告書概要(欧文)」より
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