Investigation and application of adaptation strategies of plant roots to acid soil.

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16208008
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Hokkaido University
• Principal Investigator: OSAKI Mitsuru Hokkaido University, Research Faculty of Agriculture, Professor, 大学院農学研究院, 教授 (60168903)
• Co-Investigator(Kenkyū-buntansha): SHINANO Takuro Hokkaido University, Research Faculty of Agriculture, Associate Professor, 大学院農学研究院, 助教授 (20235542) ; WATANABE Toshihiro Hokkaido University, Research Faculty of Agriculture, Assistant Professor, 大学院農学研究院, 助手 (60360939) ; KOYAMA Hiroyuki Gifu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90234921) ; TAWARAYA Keitaro Yamagata University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70179919) ; WASAKI Jun Hokkaido University, Creative Research Initiative 'Sousei' (CRIS), Associate Professor, 特任助教授 (00374728)
• Project Period (FY): 2004 – 2006
• Keywords: Acid Soil / Root Exudates / Secretory Protein / Organic Acids / Aluminum / Mucilage / Arbuscular Mycorrhiza / Phosphorus

Research Abstract

This study aimed to solve problems on acid soils for utilization as arable soils. Critical problems of acid soil are 1) toxicity of metallic ions and 2) phosphorus starvation. Some plant species have been developed some strategies to adapt on acid soils. We have shown that the several root exudates bring the tolerance to stresses on acid soils. Therefore, the secreting abilities of plant roots were investigated in this study.
A gene for secretory acid phosphatase isolated from white lupin, which is well known as a phosphorus tolerative plant, was introduced into tobacco plants. The growth and phosphorus acquisition of transgenic plant were improved from wild type plants. To investigate the secreting ability of organic acid, which can liberate inorganic phosphate from sparingly soluble forms, malate exudation of Arabidopsis was chosen as a model. It was indicated that malate release was induced by the up-regulation of malate transporter protein. The induction of malate transporter expres sion by aluminum was specific in the root tips and internal tissues of roots, suggesting that the mechanism is regulated to minimize the carbon consumption. It is concluded that site-specific regulation of the transporters is required to improve the inorganic phosphate uptake ability.
It was suggested that the specificities of mucilage, consisted of polysaccharides, in the root exudates differed among plant species. The highest affinities of mucilage of Melastoma malabathricum and maize were shown with trivalent and divalent cations, respectively. The affinity of mucilage of Melastoma malabathricum was high to aluminum ion, though the binding strength was quite lower than that of maize. It was supposed that not only the composition of sugar but also methylation of uronic acids were involved in the differences of adsorption specificity to the cation. Separation and purification of substances involved in the elongation of mycorrhizal hyphae was conducted. It was succeeded that an A2 fraction, which induced an elongation and secondary and further branching of mycorrhizal hyphae was obtained from root exudates of onion.

Research Products

• [Journal Article] Effects of enriched CO_2 levels on the nutrient uptake characteristics of Larix Kaempferi. (2007)
  • Author(s): Shinano T.
  • Journal Title: Tree Physiology 27・1 Pages: 97-104
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evolutionary control of leaf element composition in plants. (2007)
  • Author(s): Watanabe T.
  • Journal Title: New Phytologist 174・3 Pages: 516-523
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH. (2007)
  • Author(s): Fukuda T.
  • Journal Title: Plant Science 172・6 Pages: 1157-1165
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of enriched CO2 levels on the nutrient uptake characteristics of Larix Kaempferi. (2007)
  • Author(s): Shinano, T., Yamamoto, T., Tawaraya, T., Tadokoro, M., Koike, T., Osaki, M.
  • Journal Title: Tree Physiology 27(1) Pages: 97-104
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evolutionary control of leaf element composition in plants. (2007)
  • Author(s): Watanabe T, Broadley MR, Jansen S, White PJ, Takada J, Satake K, Takamatsu T, Tuah SJ, Osaki M
  • Journal Title: New Phytologist 174(3) Pages: 516-523
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH. (2007)
  • Author(s): Fukuda, T., Saito, A., Wasaki, J., Shinano, T, Osaki, M.
  • Journal Title: Plant Science 172(6) Pages: 1157-1165
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth and cell wall properties of two wheat cultivarsdiffering in their sensitivity to aluminum stress. (2006)
  • Author(s): Hossain, A.K.M.Z.
  • Journal Title: J Plant Physiol. 163・1 Pages: 39-47
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Harnessing wheat genotype x Azotobacter strain interactions for sustainable wheat production in semi arid tropics. (2006)
  • Author(s): Behl, R.K.
  • Journal Title: Tropics 15・1 Pages: 121-133
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Developmental changes of plant affecting primary photosynthate distribution in rice leaves. (2006)
  • Author(s): Shinano, T.
  • Journal Title: Photosynthetica 44・4 Pages: 591-598
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of phosphoenolpyruvate carboxylase in the adaptation of a tropical forage grass to low phosphorus acid soils. (2006)
  • Author(s): Begum, H.H.
  • Journal Title: Journal of Plant Nutrition 29・1 Pages: 35-57
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Developmental regulation of photosynthate distribution in leaves of rice. (2006)
  • Author(s): Shinano, T.
  • Journal Title: Photosynthetica 44・1 Pages: 1-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aluminium-iron interactions and growth enhancement in Melastoma malabathricum and Miscanthus sinensis dominating acid sulphate soils. (2006)
  • Author(s): Watanabe T.
  • Journal Title: Plant, Cell ＆ Environment 29・12 Pages: 2124-2132
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Internal mechanisms of plant adaptation to aluminium toxicity and phosphorus starvation in three tropical forages. (2006)
  • Author(s): Watanabe T.
  • Journal Title: Journal of Plant Nutrition 29・7 Pages: 1243-1255
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis. (2006)
  • Author(s): Hoekenga O.H.
  • Journal Title: Proc. Nat. Acad. Sci. U.S.A. 103・25 Pages: 9738-9743
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Simple identification of transgenic Arabidopsis plants carrying a single copy of the integrated gene. (2006)
  • Author(s): Kihara T.
  • Journal Title: Biosci. Biotechnol. Biochem. 70・7 Pages: 1780-1783
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi. (2006)
  • Author(s): Tawaraya K.
  • Journal Title: Journal of Plant Nutrition 29・4 Pages: 657-665
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arbuscular mycorrhizal fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions. (2006)
  • Author(s): Turjaman M.
  • Journal Title: Mycorrhiza 16・7 Pages: 459-464
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Increase in early growth and nutrient uptake of Shorea seminis seedlings inoculated with two ectomycorrhizal fungi. (2006)
  • Author(s): Turjaman M.
  • Journal Title: Journal of Tropical Forest Science 18・2 Pages: 243-249
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of panicle removal on the cytokinin level in xylem and nitrogen uptake activity of rice. (2006)
  • Author(s): Shinano T.
  • Journal Title: Soil Science and Plant Nutrition 52・3 Pages: 331-340
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves. (2006)
  • Author(s): Wasaki J.
  • Journal Title: Journal of Experimental Botany 57・9 Pages: 2049-2059
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of phosphoenolpyruvate carboxylase in the adaptation of a tropical forage grass to low phosphorus acid soils. (2006)
  • Author(s): Begum HH, Osaki M, Nanamori M, Watanabe T, Shinano T, Rao IM
  • Journal Title: Journal of Plant Nutrition 29(1) Pages: 35-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aluminium-iron interactions and growth enhancement in Melastoma malabathricum and Miscanthus sinensis dominating acid suinhate soils. (2006)
  • Author(s): Watanabe T, Jansen S, Osaki M
  • Journal Title: Plant, Cell ＆ Environment 29(12) Pages: 2124-2132
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Internal mechanisms of plant adaptation to aluminium toxicity and phosphorus starvation in three tronical forages. (2006)
  • Author(s): Watanabe T, Osaki M, Yano H, Rao IM
  • Journal Title: Journal of Plant Nutrition 29(7) Pages: 1243-1255
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth and cell wall properties of two wheat cultivars differing in their sensitivity to aluminum stress. (2006)
  • Author(s): Hossain, A.K.M.Z., Koyama, H, Hara, T.
  • Journal Title: Journal of Plant Physiology 163(1) Pages: 39-47
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis. (2006)
  • Author(s): Hoekenga, 0.H., Maron, L., Pineros, M.A., Cancado, G.M.A., Shaff, J., Kobayahsi, Y., Ryan, P.R., Dong, B, Delhaize, E., Sasaki, T., Matsumoto, H., Yamamoto, Y, Koyama., H, Kochian, L.V.
  • Journal Title: Proceedings of the National Academy of Science of the United States of America 103(25) Pages: 9738-9743
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Simple identification of transgenic Arabidopsis plants carrying a single copy of the integrated gene. (2006)
  • Author(s): Kihara, T., Zhao, C-R., Kobayashi, Y., Takita, E, Kawazu, T., Koyama, H.
  • Journal Title: Bioscience, Biotechnology and Biochemistry 70(7) Pages: 1780-1783
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi. (2006)
  • Author(s): K.Tawaraya, M.Naito, T.Wagatsuma
  • Journal Title: Journal of Plant Nutrition 29(4) Pages: 657-665
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arbuscular mycorrhizal fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions. (2006)
  • Author(s): M.Turjaman, Y.Tamai, E.Santoso, M.Osaki, K.Tawaraya
  • Journal Title: Mycorrhiza 16(7) Pages: 459-464
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tawaraya Increase in early growth and nutrient uptake of Shorea seminis seedlings inoculated with two ectomycorrhizal fungi. (2006)
  • Author(s): M.Turjaman, Y.Tamai, H.Segah, S.H.Limin, M.Osaki, K.
  • Journal Title: Journal of Tropical Forest Science 18 Pages: 243-249
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Developmental regulation of photosynthate distribution in leaves of rice. (2006)
  • Author(s): Shinano, T., Nakajima, K., Wasaki, J., Mori, H., Tei, Z., Osaki, M.
  • Journal Title: Photosynthetica 44(1) Pages: 1-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Developmental changes of plant affecting primary photosynthate distribution in rice leaves. (2006)
  • Author(s): Shinano, T., Ando, K., Okazaki, K., Osaki, M.
  • Journal Title: Photosynthetica 44(4) Pages: 591-598
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of panicle removal on the cytokinin level in xylem and nitrogen uptake activity of rice. (2006)
  • Author(s): Shinano, T., Osawa, M., Soejima, H., Osaki, M.
  • Journal Title: Soil Science and Plant Nutrition 52(3) Pages: 331-340
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves. (2006)
  • Author(s): Wasaki, J., Shinano, T., Onishi, K., Yonetani, R., Yazaki, J., Fujii, F., Shimbo, K., Ishikawa, M., Shimatani, Z., Nagata, Y., Hashimoto, A., Ohta, T., Sato, Y., Miyamoto, C., Honda, S., Kojima, K., Sasaki, T., Kishimoto, N., Kikuchi, S., Osaki, M.
  • Journal Title: Journal of Experimental Botany 57(9) Pages: 2049-2059
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Harnessing wheat genotype x Azotobacter strain interactions for sustainable wheat production in semi arid tropics. (2006)
  • Author(s): Behl, R.K., Narula, N., Vasudeva, M., Sato, A., Shinano, T., Osaki, M.
  • Journal Title: Tropics 15(1) Pages: 121-133
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 植物栄養学研究へのゲノム科学のインパクト : 講座をはじめるにあたって(総説) (2005)
  • Author(s): 小山博之
  • Journal Title: 日本土壌肥料学雑誌 76・3 Pages: 331-334
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Boron-Calcium synergically alleviates aluminum toxicity in wheat plants (Treticum aestivum L.). (2005)
  • Author(s): Hossain, A.
  • Journal Title: Soil Science and Plant' Nutrition 51・1 Pages: 43-49
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of enhanced calcium supply on aluminum toxicity in relation to cell wall properties in the root apex of two wheat cultivars differing in aluminum resistance. (2005)
  • Author(s): Hossain A.K.M.Z.
  • Journal Title: Plant Soil 276・1-2 Pages: 193-204
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evaluation of phosphorus starvation inducible genes relating to efficient phosphorus utilization in rice. (2005)
  • Author(s): Shinano, T.
  • Journal Title: Plant Soil 276・1-2 Pages: 81-87
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantitative trait loci controlling aluminum tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands). (2005)
  • Author(s): Kobayashi, Y.
  • Journal Title: Plant Cell Environ. 28・12 Pages: 1516-1524
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Living mulch with white clover improves phosphorus nutrition of maize of early growth stage. (2005)
  • Author(s): Deguchi, S.
  • Journal Title: Soil Science and Plant Nutrition 51・4 Pages: 573-576
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inoculation with the ectomycorrhizal fungi Pisolithus arhizus and Scleroderma sp. improves early growth of Shorea pinanga nursery seedlings. (2005)
  • Author(s): Turjaman, M.
  • Journal Title: New Forests 30・1 Pages: 67-73
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of root systems with respect to morphological traits and nitrogen absorbing ability in the new plant type of tropical rice lines. (2005)
  • Author(s): Samejima, H.
  • Journal Title: Journal of Plant Nutrition 28・5 Pages: 835-350
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Endogenous hormones and expression of senescence-related genes in different senescent types of maize. (2005)
  • Author(s): He, P.
  • Journal Title: Journal of Experimental Botany 56・414 Pages: 1117-1128
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Plant growth promotion abilities and micro-scale bacterial dynamics in the rhizosphere of Lupin. (2005)
  • Author(s): Unno Y.
  • Journal Title: Environmental Microbiology 7・3 Pages: 396-404
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The beneficial effect of aluminium and the role of citrate in A1 accumulation in Melastoma malabathricum. (2005)
  • Author(s): Watanabe T.
  • Journal Title: New Phytologist 165・3 Pages: 773-780
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The Function of a maize-derived phosphoenolpyruvate carboxylase (PEPC) in phosphorus-deficient transgenic rice. (2005)
  • Author(s): Begum, H.H.
  • Journal Title: Soil Science and Plant Nutrition 51・4 Pages: 497-506
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Difference in carbon distribution of initial photoassimilates between soybean and rice was revealed by 20 s pulse - 300 s chase experiments. (2005)
  • Author(s): Okazaki, K.
  • Journal Title: Soil Science and Plant Nutrition 51・6 Pages: 835-840
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aluminum accumulation in the roots of Melastoma malabathricum, an aluminum accumulating plant. (2005)
  • Author(s): Watanabe, T.
  • Journal Title: Canadian Journal of Botany 51・11 Pages: 1518-1522
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Root exudation, P acquisition and microbial diversity in the rhizosphere of Lupinus albus as affected by P supply and atmospheric CO_2 concentration. (2005)
  • Author(s): Wasaki, J.
  • Journal Title: Journal of Environmental Quality 34・6 Pages: 2157-2166
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The beneficial effect of Al and the role of citrate in Al accumulation in Melastoma malabathricum L. (2005)
  • Author(s): Watanabe T, Jansen S, Osaki M
  • Journal Title: New Phytologist 165(3) Pages: 773-780
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aluminum accumulation in the roots of Melastoma malabathricum, an aluminum accumulating plant. (2005)
  • Author(s): Watanabe T, Misawa S, Osaki M
  • Journal Title: Canadian Journal of Botany 83(11) Pages: 1518-1522
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Boron-Calcium synergically alleviates aluminum toxicity in wheat plants (Treticum aestivum L.). (2005)
  • Author(s): Hossain, A., K, M., Asgar, M.D., Tosaki, T., Koyama, H., Hara, T.
  • Journal Title: Soil Science and Plant Nutrition 51(1) Pages: 43-49
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of enhanced calcium supply on aluminum toxicity in relation to cell wall properties in the root apex of two wheat cultivars differing in aluminum resistance. (2005)
  • Author(s): Hossain A.K.M.Z, Ohno T, Koyama, H., Hara, T.
  • Journal Title: Plant and Soil 276(1-2) Pages: 193-204
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Quantitative trait loci controlling aluminum tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands). (2005)
  • Author(s): Kobayashi, Y., Furuta, Y., Ohno, T, Hara, T, Koyama, H.
  • Journal Title: Plant, Cell ＆ Environment 28(12) Pages: 1516-1524
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Living mulch with white clover improves phosphorus nutrition of maize of early growth stage. (2005)
  • Author(s): S.Deguchi, S.Uozumi, K.Tawaraya, H.Kawamoto, O.Tanaka
  • Journal Title: Soil Science and Plant Nutrition 51(4) Pages: 573-576
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inoculation with the ectomycorrhizal fungi Pisolithus arhizus and Scleroderma sp. improves early growth of Shorea pinanga nursery seedlings. (2005)
  • Author(s): M.Turjaman, Y.Tamai, H.Segah, S.H.Limin, J.Y.Cha, M.Osaki, K.Tawaraya
  • Journal Title: New Forests 30(1) Pages: 67-73
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Difference in carbon distribution of initial photoassimilates between soybean and rice was revealed by 20 s pulse-300 s chase experiments. (2005)
  • Author(s): Okazaki, K., Shinano, T., Osaki, M.
  • Journal Title: Soil Science and Plant Nutrition 51(6) Pages: 835-840
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The function of a maize-derived phosphoenolpyruvate carboxylase (PEPC) in phosphorus-deficient transgenic rice. (2005)
  • Author(s): Begum, H.H., Osaki, M., Shinano, T., Miyatake, H., Wasaki, J., Yamamura, T., Watanabe, T.
  • Journal Title: Soil Science and Plant Nutrition 51(4) Pages: 497-506
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of phosphorus starvation inducible genes relating to efficient phosphorus utilization in rice. (2005)
  • Author(s): Shinano, T., Nanamori, M., Dohi, M., Wasaki, J., Osaki M.
  • Journal Title: Plant and Soil 269(1-2) Pages: 81-87
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of root systems with respect to morphological traits and nitrogen absorbing ability in the new plant type of tropical rice lines. (2005)
  • Author(s): Samejima, H., Kondo, M., Ito, O., Nozoe, T., Shinano, T., Osaki, M.
  • Journal Title: Journal of Plant Nutrition 28(5) Pages: 835-350
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endogenous hormones and expression of senescence-related genes in different senescent types of maize. (2005)
  • Author(s): He, P., Osaki, M., Takebe, M., Shinano, T., Wasaki, J.
  • Journal Title: Journal of Experimental Botany 56(414) Pages: 1117-1128
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Plant growth promotion abilities and micro-scale bacterial dynamics in the rhizosphere of Lupin. (2005)
  • Author(s): Unno, Y., Okubo, K., Wasaki, J., Shinano, T., Osaki, M.
  • Journal Title: Environmental Microbiology 7(3) Pages: 396-404
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Root exudation, phosphorus acquisition, and microbial diversity in the rhizosphere of white Lupin as affected by phosphorus supply and atmospheric carbon dioxide concentration. (2005)
  • Author(s): Wasaki, J., Rothe, A., Kania, A., Neumann, G., Romheld, V., Shinano, T., Osaki, M., Kandeler, E.
  • Journal Title: Journal of Environmental Quality 34(6) Pages: 2157-2166
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of lupin acid phosphatase in transgenic rice for use as a phytate-hydrolyzing enzyme in animal feed. (2004)
  • Author(s): Hamada A.
  • Journal Title: Biosci. Biotechnol. Biochem 68・7 Pages: 1611-1616
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Low phosphorus tolerance mechanisms : phosphorus recycling and photosyn thate partitioning in the tropical forage grass, Brachiaria Hybrid cultivar Mulato compared with rice. (2004)
  • Author(s): Nanamori M.
  • Journal Title: Plant and Cell Physiology 45・4 Pages: 460-469
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The distribution and phylogeny of aluminium accumulating plants in the Ericales. (2004)
  • Author(s): Jansen S.
  • Journal Title: Plant Biology 6・4 Pages: 498-505
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arbuscular mycorrhizal fungal communities associated with some pioneer plants in the Lahar area of Mt. Pinatubo, Philippines. (2004)
  • Author(s): Oba, H.
  • Journal Title: Soil Science and Plant Nutrition 50・8 Pages: 1195-1203
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The distribution and phylogeny of aluminium accumulating plants in the Ericales. (2004)
  • Author(s): Jansen S, Watanabe T, Caris P, Geuten K, Lens F, Pyck N, Smets E
  • Journal Title: Plant Biology 6(4) Pages: 498-505
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of lupin acid phosphatase in transgenic rice for use as a phytate-hydrolyzing enzyme in animal feed. (2004)
  • Author(s): Hamada, A., Yamaguchi, K., Harada, M, Nikumaru, S., Wasaki, J., Shinano, T., Matsui, H., Osaki, M., Honda H.
  • Journal Title: Bioscience, Biotechnology and Biochemistry 68 (7) Pages: 1611-1616
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Low phosphorus tolerance mechanisms phosphorus recycling and photosynthate partitioning in the tropical forage grass, Brachiaria Hybrid cultivar Mulato compared with nice. (2004)
  • Author(s): Nanamori, M., Shinano, T., Wasaki, J., Yamamura, T., Rao, I.M., Osaki, M.
  • Journal Title: Plant and Cell Physiology 45 (4) Pages: 460-469
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arbuscular mycorrhizal fungal communities associated with some pioneer plants in the Lahar area of Mt. Pinatubo, Philippines. (2004)
  • Author(s): H.Oba, N.Shinozaki, H.Oyaizu, K.Tawaraya, T.Wagatsuma, W.L.Barraquio, M.Saito
  • Journal Title: Soil Science and Plant Nutrition 50(8) Pages: 1195-1203
  • Description: 「研究成果報告書概要(欧文)」より