2007 Fiscal Year Final Research Report Summary
General Understanding of Two-component Signal Transduction System
| Project/Area Number |
16208009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
MIZUNO Takeshi Nagoya University, Graduate School of Bioagricultural Sciences, Prof. (10174038)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHINO Takafumi Nagoya university, Graduate School of Bioojriculfural Sciences, Assistant Prof. (00314005)
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| Project Period (FY) |
2004 – 2007
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| Keywords | Two-component Sianal Transduction / Phosphorelay / Stress Responses / Fungi / Arabidopsis / Plant Hormones / Plant Circadian Clock |
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| Research Abstract |
(A): According to the well-documented scenario with regard to the cytokinin-mediated phosphorelay signal transduction in Arabidopsis thaliana, certain members of the type-B ARR family are crucially implicated in the regulatory networks that are primarily propagated by the cytokinin-receptors (AHKs) in response to cytokinin. Here we focused on a pair of highly homologous ARR10 and ARR12 genes by constructing an appropriate arr10 and arr12 double null mutant. The results suggested (A) that ARR10 and ARR12 are the prominent players that act redundantly in the AHK-dependent cytokinin signaling in roots.
(B) : In Arabidopsis thaliana, the flowering time is regulated through the circadian clock that measures day-length and modulates the photoperiodic CO-FT output pathway in accordance to the external coincidence model. Nevertheless, little is known about the genetic linkage between the major clock associated TOCJ, CCAI, and LHY genes and the . canonical CO-FT flowering pathway. By employing a
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set of mutants including an extremely early flowering tocl ccal thy triple mutant, here we showed that CCAI and LHY act redundantly as negative regulators for the photoperiodic flowering pathway. The results of this study suggested that the circadian clock might open an exit for a photoperiodic output pathway during daytime.
(C) : For a model filamentous fungi, Aspergillus nidulans, here we first compiled a whole list of His-Asp phosphorelay components, uncovering 15 genes for His-kinase (HK), 4 genes for response regulator (RR), and only one for histidine-containing phosphotransfer intermediate (HPt) . For all of these RR genes, a set of deletion mutants was constructed so as to create a null allele for each. When examined these mutant strains, two of them (designated as AsskA and AsrrA) showed a marked phenotype of hypersensitivity to oxidative stresses (particularly, hydrogen peroxide) .These results together are indicative of a physiological importance of the His-Asp phosphorelay signaling network involving the SskA and SrrA response regulators. Less
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Research Products
(13 results)
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[Journal Article] ARKS His-kinase regulates root elongation through an ETR1-dependent integrated abscisic acid and ethylene signaling pathway in Arabidopsis thaliana2007
Author(s)
Iwama, A., Yamashino, T., Tanaka, Y., Sakakibara, H., Kakimoto, T., Sato, S., Kato, T., Tabata, S., Nagatani, A., Mizuno, T
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Journal Title
Plant Cell Phvsiol 48
Pages: 375-380
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The SskA and SrrA response regulators are implicated in oxidative stress response of hyphae and asexual spores in the phosphorelay signaling network of Aspergillus nidulands2007
Author(s)
Hagiwara, D., Asano, Y., Mad, J., Furukawa, K., Kanamaru, K., Kato., M., Abe, K., Kobayashi, T., Yamashino, T., Mizuno, T
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Journal Title
Biosci. BiotechnoL Biochem 71
Pages: 44-847
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of NikA histidine kinase implicated in the phosphorelay signal transduction of Aspergillus nidulans with sepcial reference to fungicide responses2007
Author(s)
Hagiwara, D., Matsubayashi, Y., Marui, J., Furukawa, K., Yamashino, T., Kanamaru, K., Kato, M., Abe, K., Kobayashi, T., Mizuno, T
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Journal Title
Biosci. BiotechnoL Biochem 71
Pages: 1003-1014
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Arabidopsis clock-associated pseudo-response regulatorsPRR9, PRR7, and PRR5 coodinately and positively regulate flowering time through the canonical CONSTANS-dependent photoperiodic pathway2007
Author(s)
Nakamichi, N., Kita, M., Niinuma, K., Ito, S., Yamashino, T., Mizoguchi, T., Mizuno, T
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Journal Title
Plant Cell Physiol 48
Pages: 822-832
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic linkages between circadian clock-associated components and phytochrome-depe ndent red light-signal transduction in Arabidopsis thaliana2007
Author(s)
Ito, S., Nakamichi, N., Nakamura, Y., Niwa, Y., Kato, T., Murakami, M., Kita, M., Mizoguchi, T., Niinuma, K., Yamashino, T., Mizuno, T
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Journal Title
Plant Cell Physiol 48
Pages: 971-983
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic linkages of the circadian clock-associated genes, TOC1, CCA1, and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana2007
Author(s)
Niwa, Y., Ito, S., Nakamichi, N., Mizoguchi, T., Niinuma, K., Yamashino, T., Mizuno, T
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Journal Title
Plant Cell Physiol 48
Pages: 925-937
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of amino acid substitutions that render the Arabidopsis cytokinin receptor histidine kinase AHK4 constitutively active2007
Author(s)
Miwa, K., Ishikawa, K., Terada, K., Yamada, H., Suzuki, T., Yamashino, T., Mizuno, T
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Journal Title
Plant Cell Physiol 48
Pages: 1809-1814
Description
「研究成果報告書概要(欧文)」より
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