2007 Fiscal Year Final Research Report Summary
Highly selective extermination of diatom blooms causing labor-discoloration by using habitual viruses.
| Project/Area Number |
16208019
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Fisheries Research Agency |
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Principal Investigator |
NAGASAKI Keizo Fisheries Research Agency, Fisheries Research Agency, Natl. Res. Inst. Fish. Environ. Inland Sea, Section Chief (00222175)
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| Co-Investigator(Kenkyū-buntansha) |
TOMARU Yuji Fishers Research Agency, Researcher (10416042)
YAMAGUCHI Mineo Fishers Research Agency, Researcher (00371956)
ITAKURA Shigeru Fishers Research Agency, Researcher (10371957)
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| Project Period (FY) |
2004 – 2007
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| Keywords | virus / algal blooms / diatom / genome / single-stranded RNA / circular single-stranded DNA / plankton / algicidal microorganisms |
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| Research Abstract |
Within the four years research project, six novel diatom viruses(RsRNAV, CsNIV, CtenRNAV, CdebDNAV, CsfrRNAV, CcflDNAV) were successfully isolated and characterized. All are icosahedral viruses<40nm in diameter ; their genomes are either single-stranded DNA(ssDNA) or ssRNA. The genomes of the ssRNA viruses are linear and 3'-polyadenylated ; they have two open reading frames- a replicase polyprotein gene and a structural polyprotein gene. They showed considerable similarities to plant- or insect-infecting picomaviruses. On the other hand, CsNIV and CcflDNAV genomes are assumed to consist of a single molecule of covalently closed circular ssDNA as well as a segment of linear ssDNA. It is noticeable that the unknown genome structure was discovered in diatom-infecting viruses. Based on BLAST search results and phylogenetic analyses of RdRp domain(RNA-dependent RNA polymerase domain), both the diatom-infecting ssRNA and ssDNA viruses were shown to be novel virus groups that were only very distantly related with known viruses ; besides, significant similarities among viruses were observed within each virus group. Some of the viruses were shown to exist in natural environments at a detectable level(>3.01 infectious units/ml) ; the highest record was 3.1×10^4 infectious units/ml. These data suggest viruses have a certain impact on natural diatom populations. Furthermore, through a laboratory culture experiment, growth of laver was improved by virally inhibiting coexisting diatom proliferation. This result will provide basic data for developing a new method to prevent laver from discoloration. Further study on practical use of these diatom-infecting viruses will be eagerly expected. Due to this research project, encounter between diatomology and virology was successfully realized. We believe that the resultant data are of great value not only from the viewpoint of pure science but also practical applied science.
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[Journal Article] Genomic and phylogenetic analysis of a single-stranded RNA virus infecting Rhizosolenia setigera (Stramenopiles: Bacillariophyceae).2006
Author(s)
Shirai, Y., Takao, Y., Mizumoto, H., Tomaru, Y., Honda, D., Nagasaki, K.
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Journal Title
J. Mar. Biol. Ass. U.K. 86
Pages: 475-483
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Genomic and phylogenetic analysis of a single-stranded RNA virus infecting Rhizosolenia setigera (Stramenopiles : Bacillanophyceae).2006
Author(s)
Shirai, Y, Takao, Y, Mizumoto, H , Tomaru, Y, Honda, D , Nagasaki, K.
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Journal Title
J. Mar. Biol. Ass. U.K. 86
Pages: 475-483
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Previously unknown virus infects marine diatom2005
Author(s)
Nagasaki, K., Tomaru, Y., Takao, Y., Nishida, K., Shirai, Y., Suzuki, H., Nagumo, T.
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Journal Title
Appl. Environ. Microbiol. 71
Pages: 3528-3535
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Previously unknown virus infects marine diatom.2005
Author(s)
Nagasaki, K, Tomaru, Y, Takao, Y, Nishida, K , Shirai, Y, Suzuki, H, Nagumo, T.
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Journal Title
Appl. Environ. Microbiol. 71
Pages: 3528-3535
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Countermeasures for red tide.2004
Author(s)
Nagasaki, K.
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Journal Title
Textbook for the group training course in integrated management for coastal fisheries 2003(Japan International Cooperation Agency)
Pages: 239-245
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 珪藻感染性ウイルス研究の現状と今後の課題.2007
Author(s)
外丸裕司, 白井葉子, 藤井直幹, 尾田成幸, 鈴木秀和, 南雲 保, 高尾祥丈, 長崎慶三.
Organizer
2007年度日本海洋学会大会秋季大会
Place of Presentation
琉球大学工学部
Year and Date
2007-09-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Four diatom-infecting viruses.2006
Author(s)
Nagasaki, K., Tomaru, Y., Shirai, Y., Mizumoto, H., Takao, Y.
Organizer
SCOR Virus Meeting (Virus Ecology in Marine Systems: a Workshop on Methods)
Place of Presentation
ブリティッシュコロンビア大学
Year and Date
2006-06-02
Description
「研究成果報告書概要(和文)」より
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[Presentation] Four diatom-infecting viruses.2006
Author(s)
Nagasaki, K, Tomaru, Y, Shirai, Y, Mizumoto, H, Takao, Y.
Organizer
SCOR Virus Meeting(Virus Ecology in Marine Systems : a Workshop on Methods)
Place of Presentation
University of British Columbia
Year and Date
2006-06-02
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Previously unknown type of virus infects a bloom-forming marine diatom Chaetoceros salsugineum.2005
Author(s)
Nagasaki, K., Tomaru.Y., Takao, Y., Shirai, Y., Nishida, K., Suzuki, H., Nagumo, T.
Organizer
第4回国際藻類ウイルスワークショップ
Place of Presentation
オランダ・Het Trippenhuis
Year and Date
2005-04-21
Description
「研究成果報告書概要(和文)」より
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[Presentation] Previously unknown type of virus infects a bloom-forming marine diatom Chaetoceros salsugineum.2005
Author(s)
Nagasaki, K, Tomaru Y, Takao, Y, Shirai, Y, Nishida, K, Suzuki, H, Nagumo, T.
Organizer
The 4th Algal Virus Workshop
Place of Presentation
Het Trippenhuis(The Netherlands)
Year and Date
2005-04-21
Description
「研究成果報告書概要(欧文)」より
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