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2005 Fiscal Year Final Research Report Summary

Comprehensive understanding on plant vitalization effect of atmospheric NO_x at an ambient level as an agricultural production signal

Research Project

Project/Area Number 16208033
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MORIKAWA Hiromichi  HIROSHIMA UNIVERSITY, Graduate School of Science, Professor, 大学院理学研究科, 教授 (00089129)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Atsusi  HIROSHIMA UNIVERSITY, Graduate School of Science, Associate Professor, 大学院理学研究科, 助教授 (60270477)
TAKAHASHI Misa  HIROSHIMA UNIVERSITY, Graduate School of Science, Research Associate, 大学院理学研究科, 助手 (10294513)
Project Period (FY) 2004 – 2005
KeywordsArabidopsis thaliana / Cyclin genes / Nicotiana plumbaginifolia / Nitric oxide (NO) / nitrogen dioxide (NO2) / vegetable species / vitalization effect / agricultural production signal
Research Abstract

Globally, a total of 30 to 50 teragrams of nitrogen per year is converted to nitrogen oxides (NO_x) such as nitric oxide (NO) and nitrogen dioxide (NO_2), and released into the atmosphere, resulting in 50-150 ppb in the urban air. The major known sources of NO_x are fossil fuel combustion, biomass burning, microbial activity in soils and lightning. Atmospheric NO_x has been regarded as a major pollutant in urban air providing excess nutrient. However, in contrast to widely-accepted view on the endogenously synthesized NO_x such as NO as a plant signal, the biological function of the exogenous or atmospheric NO_x is only poorly understood. Nicotiana plumbaginifolia, Arabidopsis thaliana, lettuce (Lactuca sativa L.), sunflower (Helinthus annuus L.), cucumber (Cucumis sativus L.) and pumpkin (Cucurbita moschata Duch. ex Lam.) were grown in presence or absence of 50-200 ppb 15N-labelled NO_2 for 4-10 weeks under irrigation with a medium containing nonlabelled nitrate, after which plants were analyzed for growth parameters, element contents and ^<15>N/^<14>N isotopic ratio. It was discovered that biomass yield, total leaf area, and contents per plant of carbon (C), nitrogen (N), sulphur (S), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), free amino acids and crude proteins were almost doubled in response to prolonged the presence of NO_2 in the air. Further, the contribution of N derived from NO_2 (NO_2-N) to total plant N appeared to be negligible (0.2-14%) in these plants. Therefore, it is very likely that the atmospheric NO_x acts as a multifunctional signal to vitalize plants rather than an additional N source (plant vitalization signal effect of NO_2). Our present findings strongly suggest that atmospheric NO_x at an ambient level acts as a vital hormonal signal on plants including crops.

  • Research Products

    (31 results)

All 2007 2006 2005 2004

All Journal Article (27 results) Book (4 results)

  • [Journal Article] Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino2006

    • Author(s)
      J.Shigeto, S.Yoshihara, S.E.H.Adam, K.Sueyoshi, A.Sakamoto, H.Morikawa, M.Takahashi
    • Journal Title

      Plant Biotechnol 23

      Pages: 111-116

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino.2006

    • Author(s)
      J.Shigeto, S.Yoshihara, S.E.H.Adam, K.Sueyoshi, A.Sakamoto, H.Morikawa, M.Takahashi
    • Journal Title

      Plant Biotechnol. 23

      Pages: 111-116

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of ion beam irradiation on the regeneration and morphology of Ficus thumbergii Maxim.2005

    • Author(s)
      M.Takahashi, S.Kohama, K.Kondo, M.Hakata, Y.Hase, N.Shikazono, A.Tanaka, H.Morikawa
    • Journal Title

      Plant Biotechnol 22(1)

      Pages: 63-67

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel metabolism of nitrogen in plants.2005

    • Author(s)
      H.Morikawa, M.Takahashi, A.Sakamoto, M.Ueda-Hashimoto, T.Matsubara, K.Miyawaki, Y.Kawamura, T.Hirata, H.Suzuki
    • Journal Title

      Zeit. Naturfors 60c

      Pages: 265-271

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nocturnal uptake and assimilation of nitrogen dioxide by C3 and CAM plants2005

    • Author(s)
      M.Takahashi, D.Konaka, A.Sakamoto, H.Morikawa
    • Journal Title

      Zeit. Naturfors 60c

      Pages: 279-284

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidopsis thaliana2005

    • Author(s)
      M.Kurumata, M.Takahashi, A.Sakamoto, J.L.Ramos, A.Nepovim, T.Vanek, T.Hirata, H.Morikawa
    • Journal Title

      Zeit. Naturfors 60c

      Pages: 272-278

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] TJ1 is an orientation-independent transformation enhancer sequence2005

    • Author(s)
      H.Hokazono, M.Takahashi, A.Sakamoto, H.Morikawa
    • Journal Title

      Plant Biotechnol 22

      Pages: 137-140

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Atmospheric nitrogen dioxide gas is a plant vitalization signal to increase plant size and the contents of cell constituents2005

    • Author(s)
      M.Takahashi, M.Nakagawa, A.Sakamoto, C.Ohsumi, T.Matsubara, H.Morikawa
    • Journal Title

      New Phytol 168

      Pages: 149-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Phytoremediators from abandoned rice field2005

    • Author(s)
      M.Takahashi, S.Tsukamoto, A.Kawaguchi, A.Sakamoto, H.Morikawa
    • Journal Title

      Plant Biotechnol 22

      Pages: 167-170

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential assimilation of nitrogen dioxide by 70 taxa of roadside trees at an urban pollution level2005

    • Author(s)
      M.Takahashi, A.Higaki, M.Nohno, M.Kamada, Y.Okamura, K.Matsui, S.Kitani, H.Morikawa
    • Journal Title

      Chemosphere 61(5)

      Pages: 633-639

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of salt tolerance in ectoine-transformed tobacco plants(Nicotiana tabaccum) : photosynthesis, osmotic adjustment, and nitrogen partitioning2005

    • Author(s)
      Moghaieb R.E.A., N.Tanaka, H.Sa, R.Suwa, K.Fujita
    • Journal Title

      Plant Cell Environ 29

      Pages: 173-182

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of ion beam irradiation on the regeneration and morphology of Ficus thumbergii Maxim.2005

    • Author(s)
      M.Takahashi, S.Kohama, K.Kondo, M.Hakata, Y.Hase, N.Shikazono, A.Tanaka, H.Morikawa
    • Journal Title

      Plant Biotechnol. 22(1)

      Pages: 63-67

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Novel metabolism of nitrogen in plants.2005

    • Author(s)
      H.Morikawa, M.Takahashi, A.Sakamoto, M.Ueda-Hashimoto, T.Matsubara, K.Miyawaki, Y.Kawamura, T.Hirata, H.Suzuki
    • Journal Title

      Zeit.Naturfors. 60c

      Pages: 265-271

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nocturnal uptake and assimilation of nitrogen dioxide by C3 and CAM plants.2005

    • Author(s)
      M.Takahashi, D.Konaka, A.Sakamoto, H.Morikawa.
    • Journal Title

      Zeit.Naturfors. 60c

      Pages: 279-284

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidopsis thaliana.2005

    • Author(s)
      M.Kurumata, M.Takahashi, A.Sakamoto, J.L.Ramos, A.Nepovim, T.Vanek, T.Hirata, H.Morikawa
    • Journal Title

      Zeit.Naturfors. 60c

      Pages: 272-278

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] TJ1 is an orientation-independent transformation enhancer sequence.2005

    • Author(s)
      H.Hokazono, M.Takahashi, A.Sakamoto, H.Morikawa
    • Journal Title

      Plant Biotechnol. 22

      Pages: 137-140

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Atmospheric nitrogen dioxide gas is a plant vitalization signal to increase plant size and the contents of cell constituents.2005

    • Author(s)
      M.Takahashi, M.Nakagawa, A.Sakamoto, C.Ohsumi, T.Matsubara, H.Morikawa
    • Journal Title

      New Phytol. 168

      Pages: 149-154

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Phytoremediators from abandoned rice field.2005

    • Author(s)
      M.Takahashi, S.Tsukamoto, A.Kawaguchi, A.Sakamoto, H.Morikawa
    • Journal Title

      Plant Biotechnol. 22

      Pages: 167-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differential assimilation of nitrogen dioxide by 70 taxa of roadside trees at an urban pollution level.2005

    • Author(s)
      M.Takahashi, A.Higaki, M.Nohno, M.Kamada, Y.Okamura, K.Matsui, S.Kitani, H.Morikawa
    • Journal Title

      Chemosphere 61(5)

      Pages: 633-639

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of salt tolerance in ectoine-transformed tobacco plants (Nicotiana tabaccum) : photosynthesis, osmotic adjustment, and nitrogen partitioning.2005

    • Author(s)
      Moghaieb R.E.A., N.Tanaka, H.Saneoka, Y.Murooka, H.Ono, H.Morikawa, A.Nakamura, N.T.Nguyen, R.Suwa, K.Fujita
    • Journal Title

      Plant Cell Environ 29

      Pages: 173-182

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism.2004

    • Author(s)
      H.Morikawa, M.Takahashi, A.Sakamoto, T.Matsubara, G.Arimura, Y.Kawamura, K.Fukunaga, K.Fujita, N.Sakurai, T.Hirata, H.Ide, N.Nonoyama, H.Suzuki
    • Journal Title

      Planta 219

      Pages: 14-22

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR2004

    • Author(s)
      C.Kato, M.Takahashi, A.Sakamoto, H.Morikawa
    • Journal Title

      J. Exp. Bot 55(403)

      Pages: 1761-1763

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration2004

    • Author(s)
      A.Sakamoto, S.Sakurao, K.Fukunaga, T.Matsubara, M.Ueda-Hashimoto, S.Tsukamoto, M.Takahashi, H.Morikawa
    • Journal Title

      FEBS Lett 572

      Pages: 27-32

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Attempted reduction of 1,2,3-thiadiazole-4-carboxylates with samarium/iodine in methanol. Unexpected ring enlargement to 1,2,5-trithiapan-4,6-dicarboxylates2004

    • Author(s)
      K.Miyawaki, H.Suzuki, H.Morikawa
    • Journal Title

      Org. Biomol. Chem 2

      Pages: 2870-2873

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR.2004

    • Author(s)
      C.Kato, M.Takahashi, A.Sakamoto, H.Morikawa.
    • Journal Title

      J.Exp.Bot. 55(403)

      Pages: 1761-1763

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration.2004

    • Author(s)
      A.Sakamoto, S.Sakurao, K.Fukunaga, T.Matsubara, M.Ueda-Hashimoto, S.Tsukamoto, M.Takahashi, H.Morikawa
    • Journal Title

      FEBS Lett. 572

      Pages: 27-32

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Attempted reduction of 1,2,3-thiadiazole-4-carboxylates with samarium/iodine in methanol. Unexpected ring enlargement to 1,2,5-trithiapan-4,6-dicarboxylates.2004

    • Author(s)
      K.Miyawaki, H.Suzuki, H.Morikawa
    • Journal Title

      Org.Biomol.Chem. 2

      Pages: 2870-2873

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Methods in Biotechnology : Phytoremediation Methods and Reviews2007

    • Author(s)
      M.Takahashi, T.Matsubara, A.Sakamoto, H.Morikawa
    • Total Pages
      600
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Methods in Biotechnology : Phytoremediation Methods and Reviews ("Uptake, assimilation and novel metabolism of nitrogen dioxide in plants" ) ((ed.), N.Willey)2007

    • Author(s)
      M.Takahashi, T.Matsubara, A.Sakamoto, H.Morikawa
    • Publisher
      Humana Press (in press)
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Nitrogen and sustainable plant productivity2006

    • Author(s)
      H.Morikawa, M.Takahashi, M.Hakata, T.Matsubara, A.Sakamoto
    • Total Pages
      500
    • Publisher
      Studium Presss
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Nitrogen and sustainable plant productivity. ("Higher plants and metabolism of oxides of nitrogen" ) (Eds. R.P.Singh, N.Shankar and P.K.Jaiwal)2006

    • Author(s)
      H.Morikawa, M.Takahashi, M.Hakata, T.Matsubara, A.Sakamoto
    • Total Pages
      103-133
    • Publisher
      Studium Presss, Hostan
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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