2006 Fiscal Year Final Research Report Summary
Molecular Mechanisms of Synaptic Specificity: A Study Using Single-Cell Microarray
| Project/Area Number |
16300097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NOSE Akinao The University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (30260037)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASU Etsuko The University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (30282718)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Drosophila / Axon Guidance / Target Recognition / Microarray / Synapse / Muscle / Neural Circuit / Motor Neuron |
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| Research Abstract |
How synaptic specificity is molecularly coded in target cells is a long-standing question in neuroscience. Towards a comprehensive understanding of the mechanisms of synaptic specificity, we performed microarray analysis of two neighboring muscles in Drosophila, M12 and M13, which are innervated by distinct motor neurons. We identified a number of candidate target cues that are differentially expressed between these two muscles, including M13-enriched Wnt4, a secreted molecule of the Wnt family. In Wnt4 mutants, motor neurons that normally innervate M12 (MN12s) formed smaller synapses on M12 but. instead, formed ectopic nerve endings on M13. Furthermore, ectopic expression of Wnt4 in M12 inhibits synapse formation by MN12s. These results suggest that Wnt4 generates target specificity by preventing synapse formation on a non-target muscle. Ectopic expression of five other candidate cues expressed in M13, including Beat-IIIc, Glutactin and Sulfatasel, also inhibits synapse formation by MN12s. These results demonstrate an important role for local repulsion in generating cell-to-cell target specificity.
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[Journal Article] Regulation of layer-specific targeting by reciprocal expression of a cell adhesion molecule, capricious2006
Author(s)
Shinza-Kameda, M., Takasu, E., Sakurai, K., Hayashi, S., Nose, A.
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Regulation of layer-specific targeting by reciprocal expression of a cell adhesion molecule, Capricious.2006
Author(s)
Shinza-Kameda, M., Takasu, E., Sakurai, K., Hayashi, S., Nose, A.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Formin3 is required for assembly of the F-actin structure that mediates tracheal fusion in Drosophila.2004
Author(s)
Tanaka, H., TaTakasu, E., Aigaki, T., Kato, K., Hayashi, S., Nose, A.
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Journal Title
Developmental Biology 49
Pages: 413-425
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Formin3 is required for assembly of the F-actin structure that mediates tracheal fusion in Drosophila.2004
Author(s)
Tanaka, H., Takasu, E., Aigaki, T., Kato, K., Hayashi, S., Nose, A.
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Journal Title
Dev Biol 49
Pages: 413-425
Description
「研究成果報告書概要(欧文)」より
