2006 Fiscal Year Final Research Report Summary
Role of actin cytoskeleton in the axonal growthcone during brain development
| Project/Area Number |
16300117
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
SHIRAO Tomoaki Gunma University, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF NEUROBIOLOGY AND BEHAVIOR, PROFESSOR (20171043)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hiroyuki Gunma University, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF NEUROBIOLOGY AND BEHAVIOR, Research Associate (10334137)
HANAMURA Kenji Gunma University, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF NEUROBIOLOGY AND BEHAVIOR, Research Associate (40361365)
SHKINO Yuko UNIVERSITY OF TOKYO, INSTITUTE OF MEDICAL SCIENCE, DIVISION OF NEURONAL NETWORK, ASSOCIATE PROFESSOR (70138866)
KOJIMA Nobuhiko Gunma University, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF NEUROBIOLOGY AND BEHAVIOR, Assistant professor (80215251)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Keywords | actin / growthcone / hippocampal neuronal culture / immunofluorescence staining / actin-binding proteins / axonal growth / microtubule / 微小管 |
|---|
| Research Abstract |
During the neuronal network formation, the inhibition of axonal growthcone morphogenesis and its function make it impossible for the brain function to develop normally. In this study, in order to elucidate the regulatory mechanism of actin cytoskeleton, which is directly involved in the morphogenesis and function of axonal growthcones, we focused in the actin filament and analyzed the change in the protein network of actin-binding proteins which regulate the physicochemical and biochemical characters using primary cultured hippocampal neurons. We first analyzed the distribution of drebrin in the axonal growthcone of cultured hippocampal neurons at various developmental stages using immunocytochemistry. In the neuron of stage 2, drebrin was localized at transitional area of growthcones. The immunocytochemistry also showed that Neurabin 1 showed similar localization of in the growthcone to drebrin. We further analyzed localization of various actin-related proteins in the growthcones at stage 2. Microtubules did not invade into the transitional area. In order to discriminate drebrin E from drebrin A, we developed drebrin A-specific antibody. This antibody clearly showed that growthcone only has drebrin E. This antibody further showed that migrating neurons in the adult brain only express drebrin E. We developed the new identification method of migrating neurons in the adult brain. Using this method, we discovered migrating neurons in the piriform cortex in adult rats. We finally showed that drebrin knockdown by RNAi resulted the inhibition of axonal growth.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution : disappearance from dendritic spines and appearance in dendritic shafts2006
Author(s)
Sekino, Y., Tanaka, S., Hanamura, K., Yamazaki, H., Sasagawa, Y., Xue Y., Hayashi, K., Shirao, T.
-
Journal Title
MoL Cell. Neurosci 31
Pages: 493-504
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] AMPA receptor downscaling at the onset of Alzheimer's pathology in double knock-in mice2006
Author(s)
Chang EH, Savage MJ, Flood DG, Thomas JM, Levy RB, Mahadomrongkul, V., Shirao, T., Aoki, C., Huerta, P.T.
-
Journal Title
Proc. Natl. Acad Sci.(USA) 103
Pages: 3410-3415
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Differential effects of x-irradiation on immatur and mature hippocampal neurons in vitro.2006
Author(s)
Shirai, K., Mizui, T., Suzuki, Y., Kobayashi, Y., Nakano, T., Shirao, T.
-
Journal Title
Neurosct Lett. 399
Pages: 57-60
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] Drebrin A is a Postsynaptic Protein that Localizes in vivo to the Submembranous Surface of Dendritic Sites Forming Excitatory Synapses2005
Author(s)
Aoki, C., Sekino, Y., Hanamura, K., Fujisawa, S., Mahadomrongkul, V., Ren, Y., Shirao, T.
-
Journal Title
J. Comp. Neurol. 483
Pages: 383-402
Description
「研究成果報告書概要(欧文)」より
-
-
-
