2006 Fiscal Year Final Research Report Summary
Long-term Culture of Multicellular Spheroids Applicable to an Alternative to Animal Experiments.
| Project/Area Number |
16300165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo University of Science (2006)
National Institute for Materials Science (2004-2005) |
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Principal Investigator |
OTSUKA Hidenori Tokyo University of Science, Department of Applied Chemistry, Associate Professor, 理学部第一部応用化学科, 准教授 (00344193)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAHARA Yuji National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (20360399)
TANIGUCHI Akiyoshi National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (70256759)
KOBAYASHI Hisatoshi National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (90354266)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Biointerface / Microfabrication / Patterning / Spheroid / Cell Array / Hepatocyte / Photolithography |
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| Research Abstract |
We report here for the first time a simple and effective way to form a two-dimensional micro-array of ten thousand (100x100) hepatocyte hetero-spheroids. The hepatocyte hetero-spheroids were underlain with endothelial cells in an area of 20x20.mm, by using micro-fabricated glass substrates, coated with poly (ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver specific function, allowing us to obtain hepatocyte spheroids with a diameter of 100-μm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response. This system can be used as an alternative to animal experiments in a variety of situations in which the reliance on animals would prove impractical. Furthermore, the isolated spheroids from the array may have potential utility in constructing a tissue engineered liver by seeding spheroids into three-dimensional scaffolds.
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Research Products
(157 results)
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[Journal Article] Synthesis of Polypyridine-graft-PEG Copolymer for Protein Repellent and Stable Interface.2006
Author(s)
Satomi, Tomomi, Ueno, Koji, Fujita, Yohei, Kobayashi, Hisatoshi, Tanaka, Junzo, Mitamura, Yoshinori, Tateishi, Tetsuya, Otsuka, Hidenori
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Journal Title
Journal of Nanoscience and Nanotechnology Volume 6, Number 6
Pages: 1792-1796, (5)
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Uniformly size-controled chondrocyte spheroid array and evaluation of its function2006
Author(s)
Satomi, T., Kobayashi, H., Tanaka, J., Nagasaki, Y., Mitamura, Y., Tateishi, T., Kataoka, K., Otsuka, H.
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Journal Title
Trans. Mater. Res. Soc. Jpn. 31(3)
Pages: 655-658
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2006
Author(s)
Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka
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Journal Title
J. TISSUE ENGINEERING 12 (4)
Pages: 1061-1061
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.2006
Author(s)
Xu, L., Harada, H., Tamaki, Y-T., Matsumoto, S., Tanaka, J., Taniguchi, A
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Journal Title
J. Biol. Chem. 281
Pages: 2257-2262
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Collagen-immobilized poly (vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium.2006
Author(s)
宮下英之, 榛村重人, 小林尚俊, 田口哲志, Asano-Kato N, Y. Uchino, 加藤正文, 島崎潤, 田中順三, 坪田 一男
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Journal Title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 76B,1
Pages: 56-63
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Uniformly size-controlled chondrocyte spheroid array and evaluation of its function2006
Author(s)
Satomi, T., Kobayashi, H., Tanaka, J., Nagasaki, Y., Mitamura, Y., Tateishi, T., Kataoka, K., Otsuka, H.
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Journal Title
Trans. Mater. Res. Soc. Jpn. 31(3)
Pages: 655-658
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2006
Author(s)
Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka, J.
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Journal Title
TISSUE ENGINEERING 12 (4)
Pages: 1061-1061
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ligand Density Effect on Biorecognition by PEGylated Gold Nanoparticles : Regulated Interaction of RCA_<120> Lectin with Lactose Installed to the Distal End of Tethered PEG Strands on Gold Surface.2005
Author(s)
Takae, S., Akiyama, Y., Otsuka, H., Nakamura, T., Nagasaki, Y., Kataoka, K.
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Journal Title
Biomacromolecules 6(2)
Pages: 818-824
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2005
Author(s)
Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka, J.
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Journal Title
The 8th TESI Annual Meeting of Tissue Engineering Society International 305
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nano-and micro-fabricated surface for two-dimensionally aligned spheroid with long-term viability.2005
Author(s)
Otsuka, H., Satomi, T., Ueno, K., Takayama, G, Taniguchi, A., Kobayashi, H., Kataoka, K., Tanaka, J.
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Journal Title
The China-Japan Symposium on Regenerative Medicine 116
Description
「研究成果報告書概要(欧文)」より
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