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2006 Fiscal Year Final Research Report Summary

Long-term Culture of Multicellular Spheroids Applicable to an Alternative to Animal Experiments.

Research Project

Project/Area Number 16300165
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo University of Science (2006)
National Institute for Materials Science (2004-2005)

Principal Investigator

OTSUKA Hidenori  Tokyo University of Science, Department of Applied Chemistry, Associate Professor, 理学部第一部応用化学科, 准教授 (00344193)

Co-Investigator(Kenkyū-buntansha) MIYAHARA Yuji  National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (20360399)
TANIGUCHI Akiyoshi  National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (70256759)
KOBAYASHI Hisatoshi  National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (90354266)
Project Period (FY) 2004 – 2006
KeywordsBiointerface / Microfabrication / Patterning / Spheroid / Cell Array / Hepatocyte / Photolithography
Research Abstract

We report here for the first time a simple and effective way to form a two-dimensional micro-array of ten thousand (100x100) hepatocyte hetero-spheroids. The hepatocyte hetero-spheroids were underlain with endothelial cells in an area of 20x20.mm, by using micro-fabricated glass substrates, coated with poly (ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver specific function, allowing us to obtain hepatocyte spheroids with a diameter of 100-μm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response. This system can be used as an alternative to animal experiments in a variety of situations in which the reliance on animals would prove impractical. Furthermore, the isolated spheroids from the array may have potential utility in constructing a tissue engineered liver by seeding spheroids into three-dimensional scaffolds.

  • Research Products

    (157 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (140 results) Book (16 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Physicochemical Characterization of Densely Packed Poly(ethylene glycol) Layer for Minimizing Nonspecific Protein Adsorption2007

    • Author(s)
      Tomomi Satomi, Yukio Nagasaki, Hisatoshi Kobayashi, Tetsuya Tateishi, Hidenori Otsuka
    • Journal Title

      Journal of Nanoscience and Nanotechnology Volume 7, Number 7

      Pages: 2394-2399, (6)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stable Immobilization of an Oligonucleotide Probe on a Gold Substrate Using Tripodal Thiol Derivatives.2007

    • Author(s)
      Toshiya Sakata, Sumio Maruyama, Aiko Ueda, Hidenori Otsuka, Yuji Miyahara
    • Journal Title

      Langmuir Vol. 23, No. 5

      Pages: 2269

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Density control of Poly(ethyleneglycol) layer to regulate cellular attachment2007

    • Author(s)
      Tomomi Satomi, Yukio Nagasaki, Hisatoshi Kobayashi, Hidenori Otsuka, Kazunori Kataoka
    • Journal Title

      Langmuir 23

      Pages: 6698-6703

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cell adhesion characteristics to chemically modified silicon nitride surface2007

    • Author(s)
      Toshiya Sakata, Aiko Ueda, Yuji Miyahara
    • Journal Title

      IEEJ Transactions on Electrical and Electronic Engineering 2

      Pages: 295-300

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Direct transduction of allele-specific primer extension into electrical signal using genetic field effect transistor2007

    • Author(s)
      Toshiya Sakata, Yuji Miyahara
    • Journal Title

      Biosensors and Bioelectronics 22

      Pages: 1311-1316

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stable immobilization of an oligonucleotide probe on a gold substrate using tripodal thiol derivatives2007

    • Author(s)
      Toshiya Sakata, Sumio Maruyama, Aiko Ueda, Hidenori Otsuka, Yuji Miyahara
    • Journal Title

      Langmuir 23

      Pages: 2269-2272

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Highly sensitive detection of cytotoxicity using a modified HSP70B' promoter2007

    • Author(s)
      Wada, K-I, Taniguchi, A, Okano, T
    • Journal Title

      Biotec.Bioeng (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of Differentially Expressed Genes in Hepatocyte/Endothelial Cell Co-culture System.2007

    • Author(s)
      Takayama, G., Taniguchi, A., Okano, T.
    • Journal Title

      Tissue Eng. 13

      Pages: 159-166

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 遺伝子解析用バイオトランジスタ2007

    • Author(s)
      宮原裕二, 坂田利弥, 松元亮
    • Journal Title

      機能性材料 27(解説、総説等)

      Pages: 25-34

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Density control of Poly (ethyleneglycol) layer to regulate cellular attachment2007

    • Author(s)
      Tomomi Satomi, Yukio Nagasaki, Hisatoshi Kobayashi, Hidenori Otsuka, Kazunori Kataoka
    • Journal Title

      Langmuir 23

      Pages: 6698-6703

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Physicochemical Characterization of Densely Packed Poly (ethylene glycol) Layer for Minimizing Nonspecific Protein Adsorption2007

    • Author(s)
      Tomomi Satomi, Yukio Nagasaki, Hisatoshi Kobayashi, Tetsuya Tateishi, Hidenori Otsuka
    • Journal Title

      Journal of Nanoscience and Nanotechnology Volume 7, Number 7

      Pages: 2394-2399(6)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ACTIVATION OF LYMPHOCYTE PROLIFERATION BY BORONATE-CONTAININGPOLYMER IMMOBILISED ON SUBSTRATE : THE EFFECT OF BORON CONTENTON LYMPHOCYTE PROLIFERATION.2006

    • Author(s)
      Hidenori Otsuka, Takeshi Ikeya, Teruo Okano, Kazunori Kataoka
    • Journal Title

      European Cells and Materials 12

      Pages: 36-43

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of Polypyridine-graft-PEG Copolymer for Protein Repellent and Stable Interface.2006

    • Author(s)
      Satomi, Tomomi, Ueno, Koji, Fujita, Yohei, Kobayashi, Hisatoshi, Tanaka, Junzo, Mitamura, Yoshinori, Tateishi, Tetsuya, Otsuka, Hidenori
    • Journal Title

      Journal of Nanoscience and Nanotechnology Volume 6, Number 6

      Pages: 1792-1796, (5)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of Polypyridine-graft-PEG Copolymer at Interface2006

    • Author(s)
      Tomomi Satomi, Koji Ueno, Yohei Fujita, Hisatoshi Kobayashi, Tetsuya Tateishi, Hidenori Otsuka
    • Journal Title

      J. Jpn. Soc. Colour Mater. 79 (11)

      Pages: 475-482

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mulitiarray Formation of Cell Spheroids on a Microfabricated PEG-Brush Surface and Their Stabilization of Tissue Like Functions2006

    • Author(s)
      Otsuka, H., Kataoka, K
    • Journal Title

      Bioindustry 23(1)

      Pages: 23-30

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Construction of nano-biointerface to control cell function and behabior2006

    • Author(s)
      Otsuka, H.
    • Journal Title

      Journal of Japanese Society for Biomaterials 24(2)

      Pages: 115-121

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Contorl of Interfacial Properties for Biomedical Applications2006

    • Author(s)
      Y.Fujita, H.Otsuka
    • Journal Title

      J. Jpn. Soc. Colour Mater. 79 (12)

      Pages: 555-560

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Physicochemical characterization of the Py-g-PEG copolymer at the interface2006

    • Author(s)
      Fujita, Y., Ueno, K., Satomi, T., Yajima, H., Otsuka, H.
    • Journal Title

      Trans. Mater. Res. Soc. Jpn. 31(3)

      Pages: 649-653

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Uniformly size-controled chondrocyte spheroid array and evaluation of its function2006

    • Author(s)
      Satomi, T., Kobayashi, H., Tanaka, J., Nagasaki, Y., Mitamura, Y., Tateishi, T., Kataoka, K., Otsuka, H.
    • Journal Title

      Trans. Mater. Res. Soc. Jpn. 31(3)

      Pages: 655-658

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of polypyridine-graft-PEG copolymer for long-term stability of nonfouling character2006

    • Author(s)
      Otsuka, H., Satomi, T., Ueno, K., Mitamura, Y., Tateishi, T.
    • Journal Title

      Trans. Mater. Res. Soc. Jpn. 31(3)

      Pages: 627-631

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2006

    • Author(s)
      Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka
    • Journal Title

      J. TISSUE ENGINEERING 12 (4)

      Pages: 1061-1061

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nano-fabricated aligned spheroid for cartilage tissue engineering.2006

    • Author(s)
      H.Otsuka, T.Satomi, K.Ueno, T.Tateishi
    • Journal Title

      Biomed. Appl. Nano Technol., Adv. Sci. Technol. 53

      Pages: 67-69

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DNA sequencing based on intrinsic molecular charges2006

    • Author(s)
      Toshiya Sakata, Yuji Miyahara
    • Journal Title

      Angewandte Chemie International Edition 45

      Pages: 2225-2228

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bio-molecular embossing2006

    • Author(s)
      John P.Hulme, Jihye Gwak, Yuji Miyahara
    • Journal Title

      Journal of the American Chemical Society 128

      Pages: 390-391

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The exon 6ABC region of amelogenin mRNA contribute to increased levels of amelogenin mRNA through amelogenin protein-enhanced mRNA stabilization.2006

    • Author(s)
      Xu, L., Harada, H., Taniguchi, A
    • Journal Title

      J. Biol. Chem. 281

      Pages: 32439-32444

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.2006

    • Author(s)
      Xu, L., Harada, H., Tamaki, Y-T., Matsumoto, S., Tanaka, J., Taniguchi, A
    • Journal Title

      J. Biol. Chem. 281

      Pages: 2257-2262

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of carbocisteine on sialyl-Lewis x expression in NCI-H292 cells, an airway carcinoma cell line, stimulated with tumor necrosis factor-α.2006

    • Author(s)
      Ishibashi.Y, Inouye, Y., Okano, T., Taniguchi, A
    • Journal Title

      Eur. J. Pharmacol. 530

      Pages: 223-228

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Collagen-immobilized poly (vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium.2006

    • Author(s)
      宮下英之, 榛村重人, 小林尚俊, 田口哲志, Asano-Kato N, Y. Uchino, 加藤正文, 島崎潤, 田中順三, 坪田 一男
    • Journal Title

      JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 76B,1

      Pages: 56-63

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biocompatible high-strength glue consisting of citric acid derivative and collagen2006

    • Author(s)
      田口哲志, 斉藤浩史, 青木拓克, 内田義之, 坂根正孝, 小林尚俊, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 26,1

      Pages: 9-13

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microkeratome assisted deep lamellar keratoprosthesis2006

    • Author(s)
      Shimmura S, Miyashita H, Uchino Y, 田口哲志, 小林尚俊, Shimazaki J, 田中順三, Tsubota
    • Journal Title

      BRITISH JOURNAL OF OPHTHALMOLOGY 90,7

      Pages: 826-829

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The injectable drug delivery system for anticancer chemotherapy using the novel tissue adhesive : Characterization and in vitro evaluation2006

    • Author(s)
      Kakinoki S, Taguchi T, Kobayashi H, Tanaka J
    • Journal Title

      TISSUE ENGINEERING 12(4)

      Pages: 1077-1077, 273

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of novel crosslinkers for promoting spheroid formation2006

    • Author(s)
      Ito M, Taguchi T, Kakinoki S, Kobayashi H, Tanaka J
    • Journal Title

      TISSUE ENGINEERING 12(4)

      Pages: 1078-1078, 275

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vivo evaluation of corneal epithelium stability on collagen-immobilized PVA and amniotic-membrane hybridized PVA as an artificial cornea.2006

    • Author(s)
      Kobayashi H, Kato M, Taguchi T, Shimmura S, Miyashita H, Tsubota K, Tanaka J
    • Journal Title

      TISSUE ENGINEERING 12(4)

      Pages: 1098-1099, 337

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ナノテクノロジーと臨床検査2006

    • Author(s)
      宮原裕二
    • Journal Title

      臨床検査 50

      Pages: 1334-1340

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 電界効果トランジスタによる生体分子認識の電気的検出2006

    • Author(s)
      宮原裕二, 坂田利弥
    • Journal Title

      表面科学 27

      Pages: 13-20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of Polypyridine-graft-PEG Copolymer for Protein Repellent and Stable Interface.2006

    • Author(s)
      Satomi, Tomomi; Ueno, Koji; Fujita, Yohei; Kobayashi, Hisatoshi; Tanaka, Junzo; Mitamura, Yoshinori; Tateishi, Tetsuya; Otsuka, Hidenori
    • Journal Title

      Journal of Nanoscience and Nanotechnology Volume 6, Number 6

      Pages: 1792-1796(5)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ACTIVATION OF LYMPHOCYTE PROLIFERATION BY BORONATE-CONTAININGPOLYMER IMMOBILISED ON SUBSTRATE : THE EFFECT OF BORON CONTENTON LYMPHOCYTE PROLIFERATION.2006

    • Author(s)
      Hidenori Otsuka, Takeshi Ikeya, Teruo Okano, Kazunori Kataoka.
    • Journal Title

      European Cells and Materials Vol. 12

      Pages: ages 36-43

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mulitiarray Formation of Cell Spheroids on a Microfabricated PEG-Brush Surface and Their Stabilization of Tissue Like Functions.2006

    • Author(s)
      Otsuka, H., Kataoka, K.
    • Journal Title

      Bioindustry 23(1)

      Pages: 23-30

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Construction of nano-biointerface to control cell function and behavior2006

    • Author(s)
      Otsuka, H.
    • Journal Title

      Journal of Japanese Society for Biomaterials 24(2)

      Pages: 115-121

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Control of Interfacial Properties for Biomedical Applications2006

    • Author(s)
      Y.Fujita, H.Otsuka
    • Journal Title

      J. Jpn. Soc. Colour Mater. 79 (12)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Uniformly size-controlled chondrocyte spheroid array and evaluation of its function2006

    • Author(s)
      Satomi, T., Kobayashi, H., Tanaka, J., Nagasaki, Y., Mitamura, Y., Tateishi, T., Kataoka, K., Otsuka, H.
    • Journal Title

      Trans. Mater. Res. Soc. Jpn. 31(3)

      Pages: 655-658

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2006

    • Author(s)
      Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka, J.
    • Journal Title

      TISSUE ENGINEERING 12 (4)

      Pages: 1061-1061

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Collagen-immobilized poly (vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium.2006

    • Author(s)
      宮下英之, 榛村重人, 小林尚俊, 田口哲志, Asano-Kato N, Y. Uchino 加藤正文, 島崎潤, 田中順三, 坪田 一男
    • Journal Title

      JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 76B,1

      Pages: 56-63

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biocompatible high-strength glue consisting of citric acid derivative and collagen2006

    • Author(s)
      田中哲志, 斉藤浩史, 青木拓克, 内田義之, 坂根正孝, 小林尚俊, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYS'TEMS 26,1

      Pages: 9-13

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microkeratome assisted deep lamellar keratoprosthesis2006

    • Author(s)
      Shimmura S, Miyashita H, Uchino Y, 田中哲志, 小林尚 俊, Shimazaki J, 田中順三 Tsub ota K
    • Journal Title

      BRITISH JOURNAL OF OPHTHALMOLOGY 90,7

      Pages: 826-829

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two-dimensional Array Formation of Multi-Cellular Spheroids on Micro-patterned Polymer Brush Surfaces.2005

    • Author(s)
      Otsuka, H., Satomi, T., Hirano, A., Nagasaki, Y., Kataoka
    • Journal Title

      Key Engineering Materials vols. 288-289

      Pages: 449-452

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A Reactive Poly(ethylene glycol) Layer To Achieve Specific Surface Plasmon Resonance Sensing with a High S/N Ratio : The Substantial Role of a Short Underbrushed PEG Layer in Minimizing Nonspecific Adsorption.2005

    • Author(s)
      Uchida, K., Otsuka, H., Kaneko, M., Kataoka, K., Nagasaki, Y
    • Journal Title

      Anal. Chem. 77(4)

      Pages: 1075-1080

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tripodal Thiol-Derivatives as a Functional Interface Monolayer for Immobilization of Biomolecules.2005

    • Author(s)
      Sakata, T., Miyahara, Y., Maruyama, S., Otsuka, H.
    • Journal Title

      IEEE EMBS

      Pages: 215-218

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ligand Density Effect on Biorecognition by PEGylated Gold Nanoparticles : Regulated Interaction of RCA_<120> Lectin with Lactose Installed to the Distal End of Tethered PEG Strands on Gold Surface.2005

    • Author(s)
      Takae, S., Akiyama, Y., Otsuka, H., Nakamura, T., Nagasaki, Y., Kataoka, K.
    • Journal Title

      Biomacromolecules 6(2)

      Pages: 818-824

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 高感度センサーを目指したポリエチレングリコール修飾金コロイド担持表面の構築2005

    • Author(s)
      石井 武彦, 鈴木 祐子, 秋山 好嗣, 大塚 英典, 片岡 一則, 長崎 幸夫
    • Journal Title

      高分子論文集 62(2)

      Pages: 81-86

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of DNA recognition events using multi-well field effect devices2005

    • Author(s)
      Toshiya Sakata, Yuji Miyahara
    • Journal Title

      Biosensors and Bioelectronics 21

      Pages: 827-832

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Potentiometric detection of single nucleotide polymorphism by using a genetic field effect transistor2005

    • Author(s)
      Toshiya Sakata, Yuji Miyahara
    • Journal Title

      ChemBioChem 6

      Pages: 703-710

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DNA analysis chip based on field effect transistors2005

    • Author(s)
      Toshiya Sakata, Masao Kamahori, Yuji Miyahara
    • Journal Title

      Japanese Journal of Applied Physics 44

      Pages: 2854-2859

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Potential behavior of bio-chemically modified gold electrode for extended gate field effect Transistor2005

    • Author(s)
      Toshiya Sakata, Shinya Matsumoto, Yoshio Nakajima, Yuji Miyahara
    • Journal Title

      Japanese Journal of Applied Physics 44

      Pages: 2860-2863

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rapid and highly sensitive detection of cadmium chloride induced cytotoxicity using the HSP70B' promoter in live cells.2005

    • Author(s)
      Wada, K-I, Taniguchi, A, Xu, L, Okano, T
    • Journal Title

      Biotec.Bioeng. 92

      Pages: 410-415

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A Novel Method to Examine Hepatocyte Specific Gene Expression in a Functional Co-Culture System2005

    • Author(s)
      Kurosawa, Y., Taniguchi, A., Okano, T.
    • Journal Title

      Tissue Eng. 11

      Pages: 1650-1657

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of sialyl-Lewis x epitope expression by TNF-α and EGF in an airway carcinoma cell line.2005

    • Author(s)
      Ishibashi.Y, Inouye, Y., Okano, T., Taniguchi, A.
    • Journal Title

      Glycoconj. J. 22

      Pages: 53-62

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer.2005

    • Author(s)
      Taguchi, T., Xu, L., Kobayashi, H., Taniguchi, A., Tanaka, J.
    • Journal Title

      Biomaterials 26

      Pages: 1247-1252

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer2005

    • Author(s)
      田口哲志, 徐麗明, 小林尚俊, 谷口彰良, 片岡一則, 田中順
    • Journal Title

      BIOMATERIALS 26

      Pages: 1247-1252

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immobilization of laminin peptide in molecularly aligned chitosan by covalent bonding2005

    • Author(s)
      松田篤, 小林尚俊, 伊藤聰一郎, 片岡一則, 田中順三
    • Journal Title

      BIOMATERIALS 26

      Pages: 2273-2279

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transplantation of corneal endothelium with Descemet's membrane using a hyroxyethyl methacrylate polymer as a carrier2005

    • Author(s)
      榛村重人, 宮下英之, Konomi K, Sinozaki N, 田口哲志, 小林尚俊, 島崎潤, 田中順三, 坪田一男
    • Journal Title

      BRITISH JOURNAL OF OPHTHALMOLOGY 89,2

      Pages: 134-137

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of a laminin peptide (YIGSR) immobilized on crab-tendon chitosan tubes on nerve regeneration2005

    • Author(s)
      伊藤聰一郎, 松田篤, 小林尚俊, S.Ichinose, 四宮謙一, 田中順三
    • Journal Title

      JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 73,2

      Pages: 375-382

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Long-term effects of muscle-derived protein with molecular mass of 77 kDa (MDP77)on nerve regeneration2005

    • Author(s)
      伊藤聰一郎, Fujimoto KE, Uyeda A, 松田篤, 小林尚俊, 四宮謙一, 田中順三, Taguchi T
    • Journal Title

      JOURNAL OF NEUROSCIENCE RESEARCH 81,5

      Pages: 730-738

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] バイオトランジスタによる生体分子認識反応の高感度検出2005

    • Author(s)
      坂田利弥, 宮原裕二
    • Journal Title

      応用物理 74

      Pages: 1555-1562

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 電界効果トランジスタを用いた遺伝子解析チップ2005

    • Author(s)
      宮原裕二, 坂田利弥
    • Journal Title

      ケミカルエンジニアリング 50

      Pages: 508-514

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 半導体技術を用いたバイオチップ2005

    • Author(s)
      宮原裕二
    • Journal Title

      マテリアルインテグレーション 18

      Pages: 50-54

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 半導体遺伝子解析チップの開発と臨床診断への応用2005

    • Author(s)
      宮原裕二
    • Journal Title

      Bioclinica 20

      Pages: 32-36

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Construction of PEGylated gold colloid-assembled surface for high performance biosensor.2005

    • Author(s)
      Ishii, T.Suzuki, Y.Akiyama, Y.Otsuka, H.Kataoka, K.Nagasaki, Y.
    • Journal Title

      高感度セン サーを目指したポリエチレンダリコール修飾金コ口ロイド担持表面の構築,石井武言.鈴木祐子.秋山好 嗣.大塚英典.片岡一則.長崎幸夫,高分子論文集 62(2)

      Pages: 81-86

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ligand Density Effect on Biorecognition by PEGylated Gold Nanoparticles : Regulated Interaction of RCA120 Lectin with Lactose Installed to the Distal End of Tethered PEG Strands on Gold Surface.2005

    • Author(s)
      Takae, S.Akiyama, Y.Otsuka, H.Nakamura, T.Nagasaki, Y.Kataoka, K.
    • Journal Title

      Biomacromolecules 6(2)

      Pages: 818-824

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Reactive Poly (ethylene glycol) Layer To Achieve Specific Surface Plasmon Resonance Sensing with a High S/N Ratio : The Substantial Role of a Short Underbrushed PEG Layer in Minimizing Nonspecific Adsorption.2005

    • Author(s)
      Uchida, K.Otsuka, H.Kaneko, M.Kataoka, K.Nagasaki, Y.
    • Journal Title

      Anal. Chem. 77(4)

      Pages: 1075-1080

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two-dimensional Array Formation of Multi-Cellular Spheroids on Micro-patterned Polymer Brush Surfaces.2005

    • Author(s)
      Otsuka, H.Satomi, T.Hirano, A.Nagasaki, Y.Kataoka, K.
    • Journal Title

      Key Engineering Materials vols.288-289

      Pages: 449-452

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surface molecular engineering by mixed tethered chains.2005

    • Author(s)
      Nagasaki Y, Uchida K, Otsuka H, Kataoka K.
    • Journal Title

      THE AMERICAN CHEMICAL SOCIETY 229

      Pages: U152-U152 394 Part 1

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two-dimensional Array Formation of Multi-cellular Spheroids for Tissue Engineering.2005

    • Author(s)
      Otsuka, H.
    • Journal Title

      4th Annual Meeting of the European Tissue Engineering Society, XXXV

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of spheroid array with long-term cell viability for biomedical application : novel molecular design for cellular array fabrication.2005

    • Author(s)
      Otsuka, H., Satomi, T., Ueno, K., Suzuki, S., Enosawa, S., Kobayashi, H., Kataoka, K., Tanaka, J.
    • Journal Title

      The 8th TESI Annual Meeting of Tissue Engineering Society International 305

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nano-and micro-fabricated surface for two-dimensionally aligned spheroid with long-term viability.2005

    • Author(s)
      Otsuka, H., Satomi, T., Ueno, K., Takayama, G, Taniguchi, A., Kobayashi, H., Kataoka, K., Tanaka, J.
    • Journal Title

      The China-Japan Symposium on Regenerative Medicine 116

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly (ethylene glycol)-based 4-armed star polymer2005

    • Author(s)
      田口哲志, 徐麗明, 小林尚俊, 谷口彰良, 片岡一則, 田中順 三
    • Journal Title

      BIOMATERIALS 26

      Pages: 1247-1252

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immobilization of laminin peptide in molecularly aligned chitosan by covalent bonding2005

    • Author(s)
      松田篤, 小林尚俊, 伊藤聰 一郎, 片岡一則, 田中順三
    • Journal Title

      BIOMATERIALS 26,15

      Pages: 2273-2279

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transplantation of corneal endothelium with Descemet's membrane using a hyroxyethyl methacrylate polymer as a carrier2005

    • Author(s)
      Konomi K, Sinozaki N, 田中哲志, 小林尚俊, 島崎潤, 田中順三, 坪田一男
    • Journal Title

      BRITISH JOURNAL OF OPHTHALMOLOGY 89,2

      Pages: 134-137

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of a laminin peptide (YIGSR) immobilized on crab-tendon chitosan tubes on nerve regeneration2005

    • Author(s)
      伊藤聰一 郎, 松田篤, 小林尚俊, S.Ichinose, 四宮謙一, 田中順三
    • Journal Title

      JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 73,2

      Pages: 375-382

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Long-term effects of muscle-derived protein with molecular mass of 77 kDa (MDP77) on nerve regeneration2005

    • Author(s)
      伊藤 聰一郎, Fujimoto KE, Uyeda A, 松田篤, 小林尚俊, 四宮謙一, 田中順三
    • Journal Title

      Taguchi T, JOURNAL OF NEUROSCIENCE RESEARCH 81,5

      Pages: 730-738

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rapid and highly sensitive detection of cadmium chloride induced cytotoxicity using the HSP70B' promoter in live cells.2005

    • Author(s)
      Wada, K-I, Taniguchi, A, Xu, L, Okano, T.
    • Journal Title

      Biotec.Bioeng. 92

      Pages: 410-415

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of sialyl-Lewis x epitope expression by TNF-a and EGF in an airway carcinoma cell line.2005

    • Author(s)
      Ishibashi.Y, Inouye, Y., Okano, T., Taniguchi, A.
    • Journal Title

      Glycoconj. J. 22

      Pages: 53-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly (ethylene glycol)-based 4-armed star polymer.2005

    • Author(s)
      Taguchi, T., Xu, L., Kobayashi, H., Taniguchi, A., Tanaka, J.
    • Journal Title

      Biomaterials 26

      Pages: 1247-1252

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation of Functionally PEGylated Gold Nanoparticles with Narrow Distribution through Autoreduction of Auric Cation by α-Biotinyl-PEG-block-[poly(2-(N,N-dimethylamino)ethyl methacrylate)]2004

    • Author(s)
      Takehiko Ishii, Hidenori Otsuka, Kazunori Kataoka, Yukio Nagasaki
    • Journal Title

      Langmuir 20(3)

      Pages: 561-564

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two-dimensional Multi-array Formation of Hepatocyte Spheroids on a Micro-fabricated PEG-brush Surface2004

    • Author(s)
      Hidenori Otsuka, Akihiro Hirano, Yukio Nagasaki, Teruo Okano, Yasuhiro Horiike, Kazunori Kataoka
    • Journal Title

      ChemBioChem 6

      Pages: 850-855

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel Molecular Recognition via Fluorescent Resonance Energy Transfer Using a Biotin-PEG/Polyamine Stabilized CdS Quantum Dot2004

    • Author(s)
      Yukio Nagasaki, Takehiko Ishii, YukaSunaga, Yousuke Watanabe, Hidenori Otsuka, Kazunori Kataoka
    • Journal Title

      Langmuir 20(15)

      Pages: 6396-6400

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation of water soluble US quantum dots stabilized by functional poly(ethyleneglycol) and its application for bioassay2004

    • Author(s)
      Ishii Takehiko, Sunaga Yuka, Otsuka Hidenori, Nagasaki Yukio, Kataoka Kazunori
    • Journal Title

      J.Photopolym. Sci. Technol. 17(1)

      Pages: 95-98

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Polymer protected nanoparticles for bio-medical applications.2004

    • Author(s)
      Hidenori Otsuka, Kazunori Kataoka
    • Journal Title

      Mater. Sci. Technol. 41(4)

      Pages: 186-192

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of Aldehyde-PEG Tethered Surfaces : Influence of PEG Chain Length on the Specific Biorecognition2004

    • Author(s)
      Otsuka, H., Nagasaki, Y., Kataoka, K
    • Journal Title

      Langmuir 20(26)

      Pages: 11285-11287

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two-dimensional Array Formation of Multi-Cellular Spheroids on Micro-patterned Polymer Brush Surfaces2004

    • Author(s)
      Otsuka, H., Satomi, T., Hirano, A., Nagasaki, Y., Kataoka, K.
    • Journal Title

      Key Engineering Materials vols.288-289

      Pages: 449-452

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immobilization of oligonucleotide probes on Si3N4 surface and its application to genetic field effect transistor2004

    • Author(s)
      Toshiya Sakata, Masao Kamahori, Yuji Miyahara
    • Journal Title

      Materials Science and Engineering C 24

      Pages: 827-832

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High resolution for single-strand conformation polymorphism analysis by capillary electrophoresis2004

    • Author(s)
      Satoshi Ozawa, Kokichi Sugano, Tsuyoshi Sonehara, Shin'ichi Fukuzono, Akira Ichikawa, Noriko Fukuyama, Mariko Taylor, Yuji Miyahara, Takashi Irie
    • Journal Title

      Anal. Chem 26

      Pages: 6122-6129

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of carbocisteine on altered activities of glycosidase and glycosyltransferse and expression of Muc5ac in SO2-exposed rats.2004

    • Author(s)
      Ishibashi, Y., Kobayashi, F., Idesawa, A., Taniguchi, A., Matsuzawa, S.
    • Journal Title

      Eur. J. Pharmacol. 487

      Pages: 7-15

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 細胞シートの相互作用からバイオマテリアル表面設計を考える2004

    • Author(s)
      谷口彰良
    • Journal Title

      表面科学 25

      Pages: 30-33

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Determination of end-group structures and by-products of synthesis of poly(alpha,beta-malic acid) by direct polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 森作員子, 田口哲志, 片岡一則, 田中順三
    • Journal Title

      POLYMER DEGRADATION AND STABILITY 84,1

      Pages: 151-157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Improved Synthesis with High Yield and Increased Molecular Weight of Poly(α,β-malic acid) by Direct Polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口哲志, 片岡一則, 田中順三
    • Journal Title

      BIOMACROMOLECULES 5,1

      Pages: 169-174

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Muscle-specific protein MDP77 specifically promotes motor nerve regeneration in rats2004

    • Author(s)
      伊藤聰一郎, 松田篤, 小林尚俊, 四宮謙一, 田中順三
    • Journal Title

      NEUROSCIENCE LETTERS 360

      Pages: 175-177

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rheological evaluation of gelatin gels prepared with a citric acid derivative as a novel cross-linker2004

    • Author(s)
      青木拓克, 田口哲志, 斉藤浩史, 小林尚俊, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 787-790

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Physicochemical properties of gelatin gels prepared using citric acid derivative2004

    • Author(s)
      斉藤浩史, 田口哲志, 小林尚俊, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 781-785

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bonding of soft tissues using a novel tissue adhesive consisting of a citric acid derivative and collagen2004

    • Author(s)
      田口哲志, 斉藤浩史, 内田義之, 坂根正孝, 小林尚俊, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 775-780

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation and characterization of osteochondral scaffold2004

    • Author(s)
      田口哲志, 澤邊祐, 小林尚俊, 守吉祐介, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 881-885

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation and mechanical property of core-shell type chitosan/calcium phosphate composite fiber2004

    • Author(s)
      松田篤, 生駒俊之, 小林尚俊, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 723-728

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of activated poly(α,β-malic acid) using N-hydroxysuccinimide and its gelation with collagen as biomaterials2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口哲志, 斉藤浩史, 小松優, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 815-819

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Study on the hydrolytic degradation of poly(α,β-」 malic acid) by direct polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口哲志, 小松優, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 821-825

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Collagen immobilized PVA hydrogel - hydroxyapatite composites prepared by kneading methods as a material for peripheral cuff of artificial cornea2004

    • Author(s)
      小林尚俊, 加藤正文, 田口哲志, 生駒俊之, 宮下英之, 榛村重人, 坪田一夫, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Compounding of hydroxyapatitecrystals to molecularly aligned crab tendon chitosan : the effect of heat treatment2004

    • Author(s)
      松田篤, 笠原麻由美, 小林尚俊, 一之瀬昇, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 717-722

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] MEMS技術のバイオチップヘの応用2004

    • Author(s)
      坂田利弥, 宮原裕二
    • Journal Title

      バイオサイエンスとインダストリー 62

      Pages: 577-582

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 遺伝子トランジスタによる分子認識の電気的検出2004

    • Author(s)
      宮原裕二
    • Journal Title

      The Hitachi Scientific Instrument News 47

      Pages: 4175-4179

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation of Functionally PEGylated Gold Nanoparticles with Narrow Distribution through Autoreduction of Auric Cation by oc-Biotinyl-PEG-block-[poly(2-(N, N-dimethylamino) ethyl methacrylate)]2004

    • Author(s)
      Takehiko Ishii, Hidenori Otsuka, Kazunori Kataoka, Yukio Nagasaki.
    • Journal Title

      Langmuir 20(3)

      Pages: 561-564

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two-dimensional Multi-array Formation of Hepatocyte Spheroids on a Micro-fabricated PEG-brush Surface.2004

    • Author(s)
      Hidenori Otsuka, Akihiro Hirano, Yukio Nagasaki, Teruo Okano, Yasuhiro Horiike, Kazunori Kataoka.
    • Journal Title

      ChemBioChem 6

      Pages: 850-855

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Novel Molecular Recognition via Fluorescent Resonance Energy Transfer Using a Biotin-PEG/Polyamine Stabilized CdS Quantum Dot2004

    • Author(s)
      Yukio Nagasaki, Takehikolshii, YukaSunaga, Yousuke Watanabe, Hidenori Otsuka, Kazunori Kataoka
    • Journal Title

      Langmuir 20(15)

      Pages: 6396-6400

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation of water soluble US quantum dots stabilized by functional poly (ethyleneglycol) and its application for bioassay2004

    • Author(s)
      Ishii Takehiko, Sunaga Yuka, Otsuka Hidenori, Nagasaki Yukio, Kataoka Kazunori, J.
    • Journal Title

      Photopolym. Sci. Technol. 17 (1)

      Pages: 95-98

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polymer protected nanoparticles for bio-medical applications.2004

    • Author(s)
      Hidenori Otsuka, Kazunori Kataoka.
    • Journal Title

      Mater. Sci. Technol. 41(4)

      Pages: 186-192

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of Aldehyde-PEG Tethered Surfaces : Influence of PEG Chain Length on the Specific Biorecognition2004

    • Author(s)
      Otsuka, H.Nagasaki, Y.Kataoka, K.
    • Journal Title

      Langmuir 20(26)

      Pages: 11285-11287

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microscopic-and Nanoscopic-Characteriazation of 3D Cell Spheroid.2004

    • Author(s)
      Satomi, S., Fujita, T., Tanaka, J., Otsuka, H.
    • Journal Title

      4^<th> Asian International Symposium on Biomaterials (AISB4)

      Pages: 380

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Creation of Hepatocyte Spheroid Array for High Throughput Screening.2004

    • Author(s)
      Akihiro Hirano, Hidenori Otsuka, Yukio Nagasaki, Yasuhiro Horiike, Teruo Okano, Kazunori Kataoka.
    • Journal Title

      Mat. Res. Soc. Symp. Proc. Vol.EXS-1

      Pages: F5.2.1-F5.2.3

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Multi-array Formation of Hepatocyte Hetero-spheroids on Micro-fabricated PEG-brush Surfaces.2004

    • Author(s)
      Hidenori Otsuka, Tomomi Satomi, Jun Itadani-Harada, Yukio Nagasaki, Teruo Okano, Kazunori Kataoka.
    • Journal Title

      Mat. Res. Soc. Symp. Proc. Vol.EXS-1

      Pages: F6.2.1-F6.2.3

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High resolution for single-strand conformation polymorphism analysis by capillary electrophoresis2004

    • Author(s)
      Satoshi Ozawa, Kokichi Sugano, Tsuyoshi Sonehara, Shinichi Fukuzono, Akira Ichikawa, Noriko Fukuyama, Mariko Taylor, Yuji Miyahara, Takashi Irie
    • Journal Title

      Anal. Chem. 76

      Pages: 6122-6129

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of carbocisteine on altered activities of glycosidase and glycosyltransferse and expression of Muc5ac in S02-exposed rats.2004

    • Author(s)
      Ishibashi, Y., Kobayashi, F., Idesawa, A., Taniguchi, A., Matsuzawa, S.
    • Journal Title

      Eur. J. Pharmacol. 487

      Pages: 7-15

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 細胞シートの相互作用からバイオマテリアル表面設計を考える。2004

    • Author(s)
      谷口彰良
    • Journal Title

      表面科学 25、1、

      Pages: 30-33

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Determination of end-group structures and by-products of synthesis of poly (alpha, beta-malic acid) by direct polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 森作員子, 田口哲志, 片岡一則, 田中順三
    • Journal Title

      POLYMER DEGRADATION AND STABILITY 84,1

      Pages: 151-157

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Improved Synthesis with High Yield and Increased Molecular Weight of Poly(α,β-malic acid) by Direct Polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口哲志, 片岡一則, 田中順 三
    • Journal Title

      BIOMACROMOLECULES 5,1

      Pages: 169-174

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Muscle-specific protein MDP77 specifically promotes motor nerve regeneration in rats2004

    • Author(s)
      伊藤聰一郎, 松田篤, 小林 尚俊, 四宮謙一, 田中順三
    • Journal Title

      NEUROSCIENCE LETTERS 360

      Pages: 175-177

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rheological evaluation of gelatin gels prepared with a citric acid derivative as a novel cross-linker2004

    • Author(s)
      青木拓克, 田口 哲志, 斉藤浩史, 小林尚俊, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 787-790

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Physicochemical properties of gelatin gels prepared using citric acid derivative2004

    • Author(s)
      斉藤浩史, 田口哲志, 小林尚俊, 片 岡一則, 田中順三, S., Y.
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 781-785

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation and characterization of osteochondral scaffold2004

    • Author(s)
      田口哲志, 澤邊祐, 小林尚俊, 守吉祐介, 片岡一則, 田 中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 881-885

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation and mechanical property of core-shell type chitosan/calcium phosphate composite fiber2004

    • Author(s)
      松田篤, 生駒俊 之, 小林尚俊, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 723-728

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synthesis of activated poly (α, B-malic acid) using N-hydroxysuccinimide and its gelation with collagen as biomaterials2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口哲志, 斉藤浩史, 小松優, 片岡一則, 田中順 三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 815-819

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Study on the hydrolytic degradation of poly (α, B-)malic acid) by direct polycondensation2004

    • Author(s)
      梶山哲人, 小林尚俊, 田口 哲志, 小松優, 片岡一則, 田中順三
    • Journal Title

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 821-825

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Collagen immobilized PVA hydrogel-hydroxyapatite composites prepared by kneading methods as a material for peripheral cuff of artificial cornea2004

    • Author(s)
      小林尚俊, 加藤正文, 田口哲志, 生駒俊之, 宮下英之, 榛村重人, 坪田一夫,田中 順三
    • Journal Title

      MATERIALSSC&ENCE&ENGINEERINGC-BIOMIMETICANDSUPRAMOLECULAR SYSTEMS 24,6-8

      Pages: 729-735

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Compounding of hydroxyapatitecrystals to molecularly aligned crab tendon chitosan : the effect of heat treatment2004

    • Author(s)
      松 田篤, 笠原麻由美, 小林尚俊, 一之瀬昇, 田中順三
    • Journal Title

      MATERIALSSCIENCE&ENGINEERINGC-BIOMMETIC ANDSUPRAMOLECULARSYSTEMS 24,6-8

      Pages: 717-722

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hydroxyapatite-coated tendon chitosan tubes with adsorbed laminin peptides facilitate nerve regeneration in vivo2003

    • Author(s)
      伊藤聰一郎, 山口勇, 鈴木真弓, 市野瀬志津子, 高久田和夫, 小林尚俊, 四宮謙一, 田中順三
    • Journal Title

      BRAIN RESEARCH 993,1-2

      Pages: 111-123

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of a Nerve Scaffold Using a Tendon Chitosan Tube2003

    • Author(s)
      S.Itoh, M.Suzuki, I.Yamaguchi, K.Takakuda, 小林尚俊, K.Shinomiya., 田中順三
    • Journal Title

      ARTIFICIAL ORGANS 27,12

      Pages: 1079-1088

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hydroxyapatite-coated tendon chitosan tubes with adsorbed laminin peptides facilitate nerve regeneration in vivo2003

    • Author(s)
      伊藤聰一郎, 山口勇, 鈴木真弓, 市野瀬志津子, 高久田和夫, 小林尚俊, 四宮謙一,田中順三
    • Journal Title

      BRAIN RESEARCH 993,1-2

      Pages: 111-123

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of a Nerve Scaffold Using a Tendon Chitosan2003

    • Author(s)
      Tube, S.Itoh, M.Suzuki, I.Yamaguchi, K.Takakuda, 小林 尚俊, K.Shinomiya., 田中順三
    • Journal Title

      ARTIFICIAL ORGANS 27,12

      Pages: 1079-1088

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2007) Highly sensitive detection of cytotoxicity using a modified HSP70B' promoter.

    • Author(s)
      Wada, K-I, Taniguchi, A, Okano, T.
    • Journal Title

      Biotec.Bioeng. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] (2007) Identification of Differentially Expressed Genes in Hepatocyte/Endothelial Cell Co-culture System.

    • Author(s)
      Takayama, G, Taniguchi, A., Okano, T.
    • Journal Title

      Tissue Eng. 13

      Pages: 159-166

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A (2006) The exon 6ABC region of amelogenin mRNA contribute to increased levels of amelogenin mRNA through amelogenin protein-enhanced mRNA stabilization.

    • Author(s)
      Xu, L., Harada, H., Taniguchi
    • Journal Title

      J. Biol. Chem. 281

      Pages: 32439-32444

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A (2006) Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.

    • Author(s)
      Xu, L.Harada, H., Tamaki, Y-T., Matsumoto, S., Tanaka, J., Taniguchi
    • Journal Title

      J. Biol. Chem. 281

      Pages: 2257-2262

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A (2006) Effects of carbocisteine on sialyl-Lewis x expression in NCI-H292 cells, an airway carcinoma cell line

    • Author(s)
      Ishibashi.Y, Inouye, Y., Okano, T., Taniguchi
    • Journal Title

      stimulated with tumor necrosis factor-a. Eur. J. Pharmacol. 530

      Pages: 223-228

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] ナノテクノロジー入門シリーズII,ナノテクのための化学・材料入門,chapter2,高次構造 : ミセル,コロイド,ナノファイバー2007

    • Author(s)
      大塚英典, 片岡一則
    • Total Pages
      36-64
    • Publisher
      共立出版(2007年3月30日).
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 再生医療のためのバイオエンジニアリング.高分子界面設計と細胞・組織(スフェロイド)エンジニアリング,6章2007

    • Author(s)
      大塚英典, 片岡一則
    • Total Pages
      114-128
    • Publisher
      コロナ社(2007年4月12日).
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] バイオセンサとバイオチップ2006

    • Author(s)
      堀池靖浩, 宮原裕二
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] バイオセンサ・ケミカルセンサ辞典(DNAマイクロフルイディックシステム)(軽部征夫監修)2006

    • Author(s)
      宮原裕二
    • Publisher
      テクノシステム
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] ナノバイオ辞典(イオン感応性電界効果トランジスタ)(山根恒夫、松永是監修)2006

    • Author(s)
      宮原裕二
    • Publisher
      テクノシステム
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 遺伝子トランジスタによる分子認識の電気的検出、有機トランジスタ材料の評価と応用2005

    • Author(s)
      宮原裕二
    • Total Pages
      169-179
    • Publisher
      シー・エム・シー出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] バイオナノエレクトロニクス、ナノマテリアルハンドブック2005

    • Author(s)
      宮原裕二, 丸山純夫, 坂田利弥
    • Total Pages
      745-749
    • Publisher
      NTS出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 組織接着性実質材料,ソフトナノテクノロジー-バイオマテリアル革命- (田中順三/下村政嗣監修)2005

    • Author(s)
      小林尚俊
    • Total Pages
      32-37
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] ソフトナノテクノロジー-バイオマテリアル革命(田中順三/下村政嗣監修)2005

    • Author(s)
      伊藤聰一郎, 松田篤, 小林尚俊, 四宮謙一
    • Total Pages
      23-29
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 人工角膜研究の現状,臨床眼科/Japanese journal of clinical ophthalmology2005

    • Author(s)
      小林尚俊
    • Total Pages
      306-312
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] ナノバイオエンジニアリングマテリアル,第5章.機能性高分子表面の創製2004

    • Author(s)
      大塚英典
    • Total Pages
      11
    • Publisher
      フロンティア出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] プラスチック・機能性高分子材料辞典,第6章.細胞工学用材料2004

    • Author(s)
      大塚英典
    • Total Pages
      8
    • Publisher
      産業調査会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 図解 再生医療工学(遺伝子検出技術、立石哲也、田中順三編)2004

    • Author(s)
      宮原裕二
    • Total Pages
      274-288
    • Publisher
      工業調査会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 再生医療のデバイス化技術,図解再生医療工学2004

    • Author(s)
      小林尚俊
    • Total Pages
      233-249
    • Publisher
      工業調査会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 再生医療における材料工学の基礎,図解 再生医療工学2004

    • Author(s)
      田口哲志, 生駒俊之, 松田篤, 菊池正紀, 小林尚俊, 田中順三
    • Total Pages
      23-44
    • Publisher
      工業調査会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 骨再生とナノテクノロジー,バイオマテリアル -生体材料-2004

    • Author(s)
      小林尚俊, 生駒俊之, 菊池正紀, 田中順三
    • Total Pages
      415-421
    • Publisher
      バイオマテリアル
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 生体分子固定化用三脚型機能性界面分子及びそれを用いた遺伝子検出デバイス2005

    • Inventor(s)
      丸山純夫, 坂田利弥, 大塚英典, 宮原裕二
    • Industrial Property Rights Holder
      物質・材料研究機構
    • Industrial Property Number
      特願2005-093399
    • Filing Date
      2005-03-29
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

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