2005 Fiscal Year Final Research Report Summary
Function of dietary cyclodextrins on some copper proteins
| Project/Area Number |
16300242
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Nagoya Bunri University |
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Principal Investigator |
AOYAMA Yoritaka Nagoya Bunri University, School of Health and Human Life, Professor, 健康生活学部, 教授 (00023432)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Hiromichi Kinjo Gakuinn University, College of Human Life and Environment, Professor, 生活環境学部, 教授 (90079468)
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| Project Period (FY) |
2004 – 2005
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| Keywords | dietary cyclodextrin / liver / metallothionein / ceruloplasmin / cytochrome C oxidase / Cu, Zn-superoxidedismutase / rat / liver copper |
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| Research Abstract |
Objective : This study was performed to document the effects of dietary cyclodextrins (CDs) on mRNA levels of metallothionein (MT), Cu, Zn-superoxide dismutase (SOD), ceruloplasmin and cytochrome C oxidase containing copper in the liver. Furthermore, the protein content of MT, and the enzyme activity of SOD, ceruloplasmin, and cytochrome C oxidase were estimated.
Methodology : Male Wistar growing rats weighing 75 or 85 g were fed the basal, 5%α-, 5%β- or 5%γ-CD diet for 7 days. mRNA levels MT, SOD, ceruloplasmin and cytochrome C oxidase were estimated in the liver. Furthermore protein content of MT and the enzyme activities of SOD, ceruloplasmin, and cytochrome C oxidase in the liver were estimated. Copper and zinc content in the liver were also measured.
Results : Feeding rats with β-CD resulted in lower food intake, but the significant difference in body weight gain was not observed among groups. Dietary cyclodextrins had no effect on liver weight per 100 g body weight. Liver copper co
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ntents in the liver of rats fed the β-CD increased or tended to be higher. Hepatic mRNA levels of MT and Cu, Zn-SOD significantly increased in rats fed either β- or γ-CD. The feeding with either β- or γ-CD increased the protein contents of MT in the liver. Dietary α-CD increased the hepatic activity of Cu, Zn-SOD. While, dietary β-CD tended to increase and dietary γ-CD significantly increased hepatic mRNA levels of cytochrome C oxidase. Dietary γ-CD significantly increased hepatic activity of cytochrome C oxidase. Dietary γ-CD significantly increased hepatic mRNA levels of ceruloplasmin. Liver copper was increased by feeding the β-CD. While zinc in the liver was not changed by the dietary CDs. mRNA levels of MT in the kidney increased by feeding dietary α-,β- or γ-CDs.
Conclusion : Over all results indicate that dietary β- or γ-CD induces the increase in mRNA levels of some proteins containing copper. Thereafter it is surmised that the activities of some enzymes containing copper might be changed. Less
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Research Products
(15 results)
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[Book] 基礎栄養学2005
Author(s)
青山頼孝, 池田彩子 ほか
Total Pages
219
Publisher
アイ・ケイコーポレイション
Description
「研究成果報告書概要(和文)」より
