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2007 Fiscal Year Final Research Report Summary

Study on the development of bioassay system for organic halogenated compounds with thyroid-disrupting activity

Research Project

Project/Area Number 16310038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionShizuoka University

Principal Investigator

YAMAUCHI Kiyoshi  Shizuoka University, faculty of Science, Professor (50201827)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Akinori  Shizuoka University, Faculty of science, Assistant Professor (70432193)
TAKADA Hideshige  Tokyo University of Agriculture and Technoloy, Faculty of Agriculture, Associate Professor (70187970)
Project Period (FY) 2004 – 2007
Keywordsenvironmental pollutants / thyroid hormone / endocrine disruption / bioassay
Research Abstract

In vertebrates, thyroid hormones are essential for post-embryonic development, such as establishing the central nervous system in mammals and metamorphosis in amphibians. The present study focused on the extra-thyroidal processes that environmental chemicals are known to or suspected to target in the thyroid hormone-signaling pathway. We developed three in vitro and one in vivo bioassays, which detect chemicals interfering with thyroid hormone binding to plasma transthyretin (TTR assay), thyroid hormone binding to nuclear receptor (TR assay), induction of thyroid hormone-dependent reporter gene expression (Luc assay, and gene expression during thyroid hormone-induced amphibian metamorphosis (metamorphosis assay). Several organohalogens affect different aspects of the extra-thyroidal thyroid-hormone-signaling pathway but hardly affect thyroid hormone binding to receptors. Amphibian tadpoles are most sensitive to the effects of environmental chemicals during specific thyroid-hormone-related developmental windows. The thyroid hormone-disrupting activity of contaminants in aquatic environments in Japan and Thailand was surveyed by these bioassays. Our assays demonstrated that wastewater treatment effectively removed the TH-disrupting contaminants from wastewater in paper manufacturing plants and sewage-treatment plants. The Luc assay indicated that sewage-treatment plant effluents in Japan contained Ta-like activity whereas that in Thailand contained anti-Ta-like activity. It is likely that the three in vitro bioassays detected the same or different contaminants with TH-disrupting activity in a water sample-dependent manner. Our results indicate that a combination of several in vitro bioassays facilitates the rapid screening of environmental water samples to identify the locations of contaminants with TH-disrupting activity.

  • Research Products

    (45 results)

All 2008 2007 2006 2005 2004

All Journal Article (22 results) (of which Peer Reviewed: 10 results) Presentation (23 results)

  • [Journal Article] 3, 3' 5-L-triiodothyronine-like activity in effluents from domestic sewage-treatment plants detected by in vitro and in vivo bioassays2008

    • Author(s)
      Murata, T.
    • Journal Title

      Toxicol. Appl. Pharmacol. 226

      Pages: 309-317

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Interaction of diethylstilbestrol and ioxynil with transthyretin in chicken serum.2008

    • Author(s)
      Eguchi, R.
    • Journal Title

      Comp. Biochem. Physiol. 147 C

      Pages: 345-350

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 3,3'5-L-triiodothyronine-like activity in effluents from domestic sewage-treatment plants detected by in vitro and in vivo bioassays2008

    • Author(s)
      Tomonori, Murata
    • Journal Title

      Toxicology and Applied Pharmacology 226-3

      Pages: 309-317

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interaction of diethylstilbestrol and ioxynil with transthyretin in chicken serum2008

    • Author(s)
      Ryoji, Eguchi
    • Journal Title

      Comparative Biochemistry and Physiology C 147-3

      Pages: 345-350

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro and in vivo analysis of the thyroid system-disrupting activities of brominated phenolic and phenol compounds in Xenopus laevis.2006

    • Author(s)
      Kudo, Y.
    • Journal Title

      Toxicol. Sci. 92

      Pages: 87-95

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Thyroid system-disrupting chemicals: interference with thyroid hormone binding to plasma proteins and cellular thyroid hormone signalling pathway.2006

    • Author(s)
      Yamauchi, K.
    • Journal Title

      Rev. Environ. Health 21

      Pages: 229-251

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Uncompetitive inhibition of Xenopus laevis aldehyde dehydrogenase 1A1 by divalent cations.2006

    • Author(s)
      Rahman, F. B.
    • Journal Title

      Zool. Sci. 226

      Pages: 239-244

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo analysis of the thyroid system-disrupting activities of brominated phenolic and phenol compounds in Xenopus laevis2006

    • Author(s)
      Kudo, Y
    • Journal Title

      Toxcological Sciences 92-1

      Pages: 87-95

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Thyroid system-disrupting chemicals : interference with thyroid hormone binding to plasma proteins and cellular thyroid hormone signalling pathway2006

    • Author(s)
      Yamauchi, K
    • Journal Title

      Reviews for Environment and Health 21-4

      Pages: 229-251

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Uncompetitive inhibition of Xenopus laevis aldehyde dehydrogenase 1A1 by divalent cations2006

    • Author(s)
      Rahman, F.B
    • Journal Title

      Zoological Science 23-3

      Pages: 239-244

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of thyroid hormone-responsive genes in a chicken hepatoma cell line, LMH.2005

    • Author(s)
      Yamauchi, K.
    • Journal Title

      Zool. Sci. 22

      Pages: 1131-1135

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Characteristics of a thyroid hormone responsive reporter gene transduced into a Xenopus laevis cell line using lentivirus vector.2005

    • Author(s)
      Sugiyama, S.
    • Journal Title

      Gen. Comp. Endocrinol. 144

      Pages: 270-279

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo analysis of the thyroid disrupting activities of phenolic and phenol compounds in Xenopus laevis.2005

    • Author(s)
      Kudo, Y.
    • Journal Title

      Toxicol. Sci. 84

      Pages: 29-37

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Low-temperature arrest of the triiodothyronine-dependent transcription in Rana catesbeiana red blood cells.2005

    • Author(s)
      Murata, T.
    • Journal Title

      Endocrinology 146

      Pages: 256-264

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Seasonal expression of LHβ and FSHβ in the male newt pituitary gonadotrophs.2005

    • Author(s)
      Kano, Y.
    • Journal Title

      Gen. Comp. Endocrinol. 141

      Pages: 248-258

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Detection of thyroid system-disrupting chemicals using in vitro and in vivo screening assays in Xenopus laevis.2005

    • Author(s)
      Sugiyama, S.
    • Journal Title

      Toxcol. Sci. 88

      Pages: 367-374

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Identification of thyroid hormone-responsive genes in a chicken hepatoma cell line, LMH2005

    • Author(s)
      Yamauchi, K
    • Journal Title

      Zoological. Science 22-10

      Pages: 1131-1135

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characteristics of a thyroid hormone responsive reporter gene transduced into a Xenopus laevis cell line using lentivirus vector2005

    • Author(s)
      Sugiyama, S
    • Journal Title

      General and Comparative Endocrinology 144-3

      Pages: 270-279

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro and in vivo analysis of the thyroid disrupting activities of phenolic and phenol compounds in Xenopus laevis2005

    • Author(s)
      Kudo, Y
    • Journal Title

      Toxicological Sciences 84-1

      Pages: 29-37

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Low-temperature arrest of the triiodothyronine-dependent transcription in Rana catesbeiana red blood cells2005

    • Author(s)
      Murata, T
    • Journal Title

      Endocrinology 146-1

      Pages: 256-264

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Seasonal expression of LHβ and FSHβ in the male newt pituitary gonadotrophs2005

    • Author(s)
      Kano, Y
    • Journal Title

      General and Comparative Endocrinology 141-3

      Pages: 248-258

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of thyroid system-disrupting chemicals using in vitro and in vivo screening assays in Xenopus laevis2005

    • Author(s)
      Sugiyama, S
    • Journal Title

      Toxcological Sciences 88-2

      Pages: 367-374

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 下水処理場排水中に存在する甲状腺撹乱物質のバイオアッセイによる検出2007

    • Author(s)
      古藤泰弘
    • Organizer
      日本環境ホルモン学会
    • Place of Presentation
      大宮
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Detection of thyroid disrupting activity in sewage-treatment water by bioassays2007

    • Author(s)
      Kotoh, Y
    • Organizer
      The 10th annual meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      Omiya
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Characteristics of a thyroid hormone responsitive luciferase gene transduced into a frog cell line using a viral vector.2007

    • Author(s)
      Kotoh, Y.
    • Organizer
      The 3rd Korean-Japanese Workshop
    • Place of Presentation
      浜松
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Characteristics of a thyroid hormone responsitive luciferase gene transduced into a frog cell line using a viral vector2007

    • Author(s)
      Kotoh, Y
    • Organizer
      The 3rd Korean-Japanese Workshop
    • Place of Presentation
      Hamamatsu
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of thyroid disrupting activities of Environmental compounds using various assays.2007

    • Author(s)
      Rahman, F. B.
    • Organizer
      日本放射化学会年会
    • Place of Presentation
      静岡
    • Year and Date
      2007-09-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of thyroid disrupting activities of environmental compounds using various assays2007

    • Author(s)
      Rahman, F.B
    • Organizer
      The 51st, annual meeting of Radiochemistry
    • Place of Presentation
      Shizuoka
    • Year and Date
      2007-09-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ハロゲン化合物によるアフリカツメガエル甲状腺系かく乱作用機構の検討2006

    • Author(s)
      石原顕紀
    • Organizer
      日本環境ホルモン学会第9回研究発表会
    • Place of Presentation
      東京
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of halogenated conpounds on amphibian thyroid system2006

    • Author(s)
      Ishihara, A
    • Organizer
      The 7th annual meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アフリカツメガエル甲状腺系の及ぼすハロゲン化合物の影響2006

    • Author(s)
      石原顕紀
    • Organizer
      日本動物学会第77回大会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of halogenated conpounds on the thyroid system in Xenopus laevis2006

    • Author(s)
      Ishihara, A
    • Organizer
      The 77th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Shimane
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 甲状腺系撹乱化学物質の標的細胞への影響-両生類を用いた研究-2006

    • Author(s)
      山内清志
    • Organizer
      日本動物学会第77回大会
    • Place of Presentation
      島根
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of thyroid disrupting chemicals on target cells in amphibians2006

    • Author(s)
      Yamauchi, K
    • Organizer
      The 77th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Shimane
    • Year and Date
      2006-09-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 細胞膜に存在する甲状腺ホルキン取り込み機構に影響を与える化学物質の解析2004

    • Author(s)
      山内清志
    • Organizer
      日本環境ホルモン学会第7回研究発表会
    • Place of Presentation
      名古屋
    • Year and Date
      2004-12-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] アフリカツメガエル培養細胞を用いた甲状腺ホルモン作用を撹乱する化学物質の検索2004

    • Author(s)
      杉山慎一朗
    • Organizer
      日本環境ホルモン学会第7回研究発表会
    • Place of Presentation
      名古屋
    • Year and Date
      2004-12-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] フェノール類によるアフリカツメガエル幼生の変態および甲状腺ホルモン応答遺伝子の抑制2004

    • Author(s)
      工藤裕美子
    • Organizer
      日本環境ホルモン学会第7回研究発表会
    • Place of Presentation
      名古屋
    • Year and Date
      2004-12-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of chemicals on cellular uptake of thyroid hormone2004

    • Author(s)
      Yamauchi, K
    • Organizer
      The 7th annual meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      Nagoya
    • Year and Date
      2004-12-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Supression of thyroid hormone-induced metamorphosis and target genes by phenolic compounds2004

    • Author(s)
      Kudo, Y
    • Organizer
      The 7th annual meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      Nagoya
    • Year and Date
      2004-12-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Detection of chemicals with thyroid system-disrupting activity2004

    • Author(s)
      Sugiyama, S
    • Organizer
      The 7th annual meeting of Japan Society of Endocrine Disrupters Researchin Xenopus laevis cultue cells
    • Place of Presentation
      Nagoya
    • Year and Date
      2004-12-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 低温処理によって生じるウシガエル変態抑制の分子機構2004

    • Author(s)
      村田智紀
    • Organizer
      日本動物学会第75回大会
    • Place of Presentation
      神戸
    • Year and Date
      2004-09-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] フェノール類によるツメガエル幼生の変態及び甲状腺ホルモン応答遺伝子発現への影響2004

    • Author(s)
      工藤裕美子
    • Organizer
      日本動物学会第75回大会
    • Place of Presentation
      神戸
    • Year and Date
      2004-09-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular mechanism of low-temperature induced arrest of amphibian Metamorphosis2004

    • Author(s)
      Murata, T
    • Organizer
      The 75th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Kobe
    • Year and Date
      2004-09-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of phenolic compounds on a frog metamorphosis and thyroid Hormone induced genes.2004

    • Author(s)
      Kudo, T
    • Organizer
      The 75th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Kobe
    • Year and Date
      2004-09-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of chlorinated phenol compounds on the thyroid system through their binding to transthyretin and thyroid receptor.2004

    • Author(s)
      Yamauchi, K
    • Organizer
      The Fifth congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2004-03-30
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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