2007 Fiscal Year Final Research Report Summary
Comparative genomics of model plant and non-model plants based on metabolome analysis
| Project/Area Number |
16310137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Chiba University |
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Principal Investigator |
YAMAZAKI Mami Chiba University, Graduate School of Pharmaceutical Sciences, Associate professor (70222370)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Kazuki Chiba University, Graduate School of Pharmaceutical Sciences, Professor (00146705)
NOJI Masaaki Tokushima Bunri University, Faculty of Pharmaceutical Sciences, Associate professor (80271534)
TAKAYAMA Hiromitsu Chiba University, Graduate School of Pharmaceutical Sciences, Professor (90171561)
KITAJIMA Mariko Chiba University, Graduate School of Pharmaceutical Sciences, Associate professor (60195261)
ARITA Masanori Tokyo University, Graduate School of Frontier Sciences, Associate professor (10356389)
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| Project Period (FY) |
2004 – 2007
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| Keywords | anthocyanin / camptothecin / quinolizidine alkaloid / secondary metabolism / comparative genetics / biosynthesis |
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| Research Abstract |
In this study, the networks of gene expression and metabolism in plant were investigated by the comparative genetics between a model plant, and medicinal and/or crop plants. The chemotypes, transgenic mutants or cultured tissues that differ in secondary metabolites, were used as the experimental materials to profile genes concerning on the control mechanism of secondary metabolisms. (1) Anthocyanin production and accumulation: The specifically induced genes in PAP1-over-expressing arabidopsis were profiled and function unknown genes were obtained. By metabolome analysis of knockout lines of these genes, the function of a new BAHD acyltransferase was identified. From the comparison between GST homolgues expressed in pigmented perilla and PAP1 arabidopsis, functional difference in seed coat pimentation was clarified. (2) Camptothecin production: The candidate genes for the biosynthesitic pathway of camptothecin were profiled from the hairy roots of Ophiorrhiza pumila. The metabolome changes in RNAi hairy roots were analyzed and gene functions were predicted. The amino acid sequences of Topoisomerase I, the target protein of camptothecin, were compared among of several plants and human. The amino acid substitutions which cause the self-resistance against camptothecin were found in camptothecin-producing plants. (3) Quinolizidine alkaloid (Lupin alkaloid): Using alkaloid-containing 'Bitter form' and alkaloid-free 'Sweet form' of Lupinus angustifolius, 'bitter-specific' genes were profiled. Using the recombinant protein expressed in E. coli , enzymatic characterization of a decarboxylase was performed. Comparison of amino acid sequences of this enzyme among quinolizidine alkaloid containing species, several common substitution were found.
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[Journal Article] Convergent evolution in the BAHD family of acyl transferases; identification and characterisation of anthocyanin acyl transferases from Arabidopsis thaliana2007
Author(s)
Luo, J., Nishiyama, Y., Fuell, C., Taguchi, G., Elliott, K., Hill, L., Tanaka, Y., Kitayama, M., Yamazaki, M., Bailey, P., Parr, A., Michael, A. J., Saito K. and Martin, C.
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Journal Title
Plant J. 50(4)
Pages: 678-695
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional genomics by integrated analysis of metabolome and transcrptome of Arabidospsis plants overexpressing a MYB transcription factor2005
Author(s)
Tohge, T., Nishiyama, Y., Yokota-Hirai, M., Yano, M., Nakajima, J., Awazuhara, M., Inoue, E., Takahashi, H., Goodenowe, D. B., Kitayama, M., Noji, M., Yamazaki, M. and Saito, K.
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Journal Title
Plant J 42
Pages: 218-235
Description
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[Journal Article] Biosynthesis of camptothecin. In silico and in vivo tracer study from [1-^<13> C] glucose2004
Author(s)
Yamazaki, Y., Kitajima, M., Arita, M., Takayama, H., Sudo, H., Yamazaki, M., Aimi N. and Saito, K.
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Journal Title
Plant Physiol 134
Pages: 161-170
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mechanistic studies on three 2-oxoglutarate dependent oxygenases of flavonoid biosynthesis: Anthocyanidin synthase, flavonol synthase, and flavanone 3b-hydroxylase2004
Author(s)
Turnbull, J. J. , Nakajima, J. , Welford, R. W. D. , Yamazaki, M. , Saito K. , and Schofield C. J.
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Journal Title
J. Biol. Chem 279
Pages: 1206-1216
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Camptothecin production by in vitro cultures of Ophiorrhiza liukiuensis and O. kurolwai2004
Author(s)
Asano, T., Watase, I., Sudo, H., Kitajima, M., Takayama, H., Aimi, N., Yamazaki, M., Saito, K.
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Journal Title
Plant Biotech 21
Pages: 275-281
Description
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[Book] Concepts in Plant Metabolomics, Eds. by Basil J. Nikolau, Eve Syrkin Wurtele, eds: Springer Verlag, Heidelberg2007
Author(s)
Takayuki Tohge, Yasutaka Nishiyama, Masami Hirai-Yokota, Mitsuru Yano, Junichiro Nakajima, Motoko Awazuhara, Inoue, E., Hideki Takahashi, Dian B. Goodenowe, Masahiko Kitayama, Masaaki Noji, Mami Yamazaki, Kazuki Saito
Total Pages
159-168
Publisher
Identification of Genes Involved in Anthocyanin Accumulation by Integrated Analysis of Metabolome and Transcriptome in Pap1-Overexpressing Arabidopsis Plants
Description
「研究成果報告書概要(欧文)」より
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