2005 Fiscal Year Final Research Report Summary
Manipulation of Genes in the Cell Using Glycoviruses and Signal-Amplifying Probes
| Project/Area Number |
16350087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
AOYAMA Yasuhiro Kyoto University, Graduate School of Engineering, Professor, 工学研究科, 教授 (00038093)
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| Co-Investigator(Kenkyū-buntansha) |
SANDO Shinsuke Kyoto University, Graduate School of Engineering, Research Associate, 工学研究科, 助手 (20346084)
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| Project Period (FY) |
2004 – 2005
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| Keywords | glycovirus / cerasome / riboregulator / gene delivery / gene sensing / molecular beacon / read-through / sense codon |
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| Research Abstract |
1.Gene Delivery Using Glycoviruses
The viral size of 〜50nm was found to be best for cellular uptake. We found glycoviruses having three galactose moieties fulfill this requirement.
2.Gene Delivery Using Cerasomes.
Cerasomes (ceramic-coated liposomes) turned out to be free from fusion when binding to DNA and can thus be used as an excellent, viral size-keeping gene carrier.
3.Gene Sensing using Riboregulator
Molecular beacon-type riboregulator probes express particular reporter protein in the presence of a particular gene as a target and can thus be used as a signal-amplifying gene sensor.
4.Genotype-Phenotype Coupling
We devised a read-through ribosome-display strategy, thereby tagging the expressed protein with the mRNA that codes the former.
5.Sense Codon Suppression with Nonnatural Amino Acids
We found a simple method to introduce a nonnatural amino acid into sense codon using an inhibitor of the aminoacyl-tRNA synthetase.
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